Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,589	0	A	T	46.3%	65.6 / 31.3	54	L135F (TTA→TTT)	yegJ	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (8/21);  new base T (25/0);  total (33/21)
Fisher's exact test for biased strand distribution p-value = 1.12e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC  >  NC_000913/2151443‑2151735                                                                                                                                                    |                                                                                                                                                    aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTgg                                                                                                                                                 <  2:402934/151‑1 (MQ=255)          ggCGTAATCAGCAATGGCCCTAGTATTGTTACAAACGTAGAATATGGGCAGGAGTGGAAAATCAAGAAAGAAGATATTACAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑TCCATTGATCCTTTATTGGTCACCTa                                                                                                                                          <  1:856222/149‑1 (MQ=255)          ggCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTAt                                                                                                                                         <  1:216328/150‑1 (MQ=255)           gCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑TTCATTTATCCTTTTTTTGTcccc                                                                                                                                            >  1:690784/1‑146 (MQ=255)           gCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACAATTATACTTTTTTTGTCACCTAt                                                                                                                                         >  2:217831/1‑149 (MQ=255)                aTCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑ACCATTTATTCTTTTTTTGTCACCTATCCcaaa                                                                                                                                   >  2:798136/1‑150 (MQ=255)                aTCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACATTTATTCTTTTTTTGTTACCTATTCcaaa                                                                                                                                   >  1:1035224/1‑150 (MQ=255)                     cAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACATTTATTCTTTTTTTTTTACCCATCCAaaaaaa                                                                                                                                >  1:922077/1‑148 (MQ=255)                      aaTGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTTATCCTTTTTTTGTTACCTATCCaaaaaaaaaa                                                                                                                             >  2:434768/1‑150 (MQ=255)                       aTGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACCTTTATTCTTTTTTTGTCACCCATCCTAaaaaaaaa                                                                                                                             >  2:161181/1‑149 (MQ=255)                       aTGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGATATAACCATTATACCTTTTTTGTTAACC‑ATCCaaaaaaaaaa                                                                                                                             >  2:225487/1‑149 (MQ=255)                         gACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACATTGATTCTTTTTTTGTTCCCTATCCAAAAAAAAAAGcc                                                                                                                          >  2:745710/1‑150 (MQ=255)                                gTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑TAAAATTATTCTTTTTTTTTTACCTATTAAAAAAAAAAAGGcgacga                                                                                                                     >  1:579230/1‑148 (MQ=255)                                    tGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTTATTCTTTTTTTGTTACCTATCCCAAAAAAAAAACcgacga                                                                                                                     >  2:418014/1‑144 (MQ=255)                                     gTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAaga                                                                                                              <  1:311810/150‑1 (MQ=255)                                       tACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGc                                                                                                            <  2:701385/150‑1 (MQ=255)                                        aCAAACGTGGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCaa                                                                                                          <  1:162801/151‑1 (MQ=255)                                          aaaCGTAGAATATGGTCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACCTTTATTCCTTTTTTGTTACCCATCCAAAAAAAAAAAACGGCGAGCTAAAAGGaaa                                                                                                         >  2:433693/1‑150 (MQ=255)                                           aaCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAt                                                                                                       <  1:777289/151‑1 (MQ=255)                                           aaCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGGTGT‑ATCATTTATCATTTTTTGGTTACCCCTCCAAAAAAAGAAAAGGACGAACAAAGAGCaaaa                                                                                                        >  1:460422/1‑150 (MQ=255)                                              gTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAg                                                                                                    <  2:804576/151‑1 (MQ=255)                                                  aaTATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACATTTATCCTTTTTTTGTCACCTATCCAAAAAAAAAAACCCACGAGCCAAGAGCAAAAATAGtt                                                                                                  >  2:1245/1‑149 (MQ=255)                                                   atatGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGATTT‑ATCAATGGTCCTTTTTTTGTTTACTAACCAAAAAAAAAAAGcgacgaaactagaacgaaa                                                                                                        >  1:428265/1‑129 (MQ=255)                                                       ggCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑ATCCTTTATCCTTTTTTTGTGTCCTTTTCCAAAAAAAAAACCGACGAGCTAAAAGCAAAAATAATTTGCtta                                                                                            >  2:754358/1‑148 (MQ=255)                                                        gCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTTATCCTTTTTTTGTCACCCATCCAAAAAAAAAAGCCGACGAGCTAAAAGCAAAAATAGTTTGCTaa                                                                                            >  2:75365/1‑149 (MQ=255)                                                          cAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACACTTTTTCTTTTTTTGTGACCCATCCAAAAAAAAAAAACGACGAGCTAAAAGGAAAAATATTTTGCTaa                                                                                            >  1:1041256/1‑147 (MQ=255)                                                              aGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACCTTTATTCTTTTTTTGTTACCTATCCCAAAAAAAAAAACGACGAGCTAAAAGCAAAAATAATTTGCTTAtaaacc                                                                                      >  1:834258/1‑144 (MQ=255)                                                                  gAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTtata                                                                                <  2:222473/151‑1 (MQ=255)                                                                   aaaaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTatat                                                                               <  1:222030/151‑1 (MQ=255)                                                                   aaaaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCAATTGTTCTTTTTTTGTAACCCATCCCAAAAAAAAAAACGACGAGGTAAAAGGAAAATTAGGTTGTTTATCAGc                                                                                       >  2:160142/1‑143 (MQ=255)                                                                     aaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTTATTCTTTTTTTGTTACCCATCCCAAAAAAGAAGACGACGAGCCAAAAGCAAAAATATTTTGCTAATCAGCCGGTAtattt                                                                             >  2:103918/1‑151 (MQ=255)                                                                        cAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACATTTATTCTTTTTTTGTGACCCATCCCAAAAAAGAAAACGCCGAGCTAAGAGGAAAAATAATTTGCTAATTAACCGTTAtttttttt                                                                          >  2:622346/1‑151 (MQ=255)                                                                            aaagaaGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACATTTATCCTTTTTTTGTTACCTATCCAAAAAAAAAAAACGACCAGCCAAGAGCAAAAATAATTCGCTAATCAGCCCGTTTAtttttttttt                                                                      >  2:879961/1‑151 (MQ=255)                                                                              agaagaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTaa                                                                    <  2:360682/151‑1 (MQ=255)                                                                                        tCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGt                                                           <  1:162800/150‑1 (MQ=255)                                                                                         cAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGtt                                                          <  2:570518/150‑1 (MQ=255)                                                                                         cAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGtt                                                          <  2:842382/150‑1 (MQ=255)                                                                                           gACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑aa                                                      <  2:3807/151‑1 (MQ=255)                                                                                           gACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGGTTT‑AACAATTATTCTTTTTTTGTCACCCATACCAAAAAAAAAAACGACGAGCTAAAAGCAAAAATAATTTTCTAATCAACCCTTATATTTTTTTTTTTAAATTGCgg                                                            >  1:185069/1‑146 (MQ=255)                                                                                            aCTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AAt                                                     <  2:720105/151‑1 (MQ=255)                                                                                                 aTGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AAttgttg                                                <  1:732704/151‑1 (MQ=255)                                                                                                       aCGCGCGGGGATAAAATTTACGGCGGGTAT‑TCCACTTATCCTTTTTTGGGGACCTTTCCCAAAAAAGAAAACCACGAGGTAAAAGGAAAAATATTTCCGTAATTACACCTCTTATTTATTGTTTTAACTCGCGGTTgttatttttttgt                                             >  2:385317/1‑137 (MQ=255)                                                                                                           gcgGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAAcct                                      <  1:225400/151‑2 (MQ=255)                                                                                                            cgGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAACCGc                                     <  1:768127/151‑1 (MQ=255)                                                                                                                                   at‑aCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGGTG‑AATTGTTGTCTTCAAACGCCATGAGAAAATCAATCTGccc                >  1:164468/1‑149 (MQ=255)                                                                                                                                    t‑aCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑GATTGTTGTCTTTAAACGCCATGAGAAACTCACTCTGCCcac              >  2:666932/1‑150 (MQ=255)                                                                                                                                      aCCATTGATCCTTTATTGGACACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAATTGTT‑TCTTCAACCGACATTAGAAACTCACTTTGccccc              >  2:902768/1‑147 (MQ=255)                                                                                                                                       ccATTGTTCTTTTATTGGTCCCCTACCAAAAAAAAAACGCCGACGACCAAAGAGAAAAATTATTCCGCTAACCACCCTTTATTTTTATTGTTTAACCGGGCGTTTA‑AATTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCcac              <  1:217748/148‑1 (MQ=255)                                                                                                                                        cATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCcaca             >  1:89832/1‑148 (MQ=255)                                                                                                                                           tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTTAACCGCCATGGGAAACCCACTCTTCCCCCAGCGcc        >  1:40759/1‑150 (MQ=255)                                                                                                                                            gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGcc        >  2:339461/1‑149 (MQ=255)                                                                                                                                               cttttATTGGTCACCTACCAAAAAAAAGAGGCCGACGAGCAAAGAGAAAAATTAGTTCGCTAACCACCCTTTATTTTTATTTTTAAACCGGGCGTTTA‑ATTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGt     <  1:964201/147‑1 (MQ=255)                                                                                                                                                cTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTTTCTTCAACCCCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc  >  2:30321/1‑151 (MQ=255)                                                                                                                                                cTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAACCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGcc  >  2:963306/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC  >  NC_000913/2151443‑2151735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑