Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,781,6450AC50.0% 51.2 / 13.1 48V361G (GTT→GGT) ypjAadhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base A (17/7);  new base C (0/24);  total (17/31)
Fisher's exact test for biased strand distribution p-value = 1.63e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATAATTGTTGTGTCAGCTTTCCCGCCGCTTTTGATATTTTGCGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACATTGTCTGC  >  NC_000913/2781499‑2781790
                                                                                                                                                  |                                                                                                                                                 
atatAATTGTTGTGTCAGCTTTCCCGCCGCTTTTGATATTTTGCGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAAccc                                                                                                                                                <  2:724900/150‑1 (MQ=255)
atatAATTGTTGTGTCAGCTTTCCCGCCGCTTTTGATATTTTGCGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAAc                                                                                                                                                  <  1:93493/148‑1 (MQ=255)
    aaTTGTTGTGTCAGCTTTCCCGCCGCTTTTGATATTTTGCGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGcc                                                                                                                                            >  1:226420/1‑150 (MQ=255)
    aaTTGTTGTGTCAGCTTTCCCGCCGCTTTTGATATTTTGCGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGcc                                                                                                                                            >  2:948358/1‑150 (MQ=255)
        gttgtGTCAGCTTTCCCGCCGCTTTTGATATTTTGCGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTAt                                                                                                                                       >  1:894812/1‑151 (MQ=255)
             gtCAGCTTTCCCGCCGCTTTTGATATTTTGCGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTAtt                                                                                                                                  >  2:433538/1‑151 (MQ=255)
                     ccccGCCGTTTTTATTATTTTGCTTTCCGCTGTTGATATTGGTGCTTGTGGCTATGCCATAATTATTAATTTTCTGTGTCCCACCATTAATTATGTTTTTTGTCGCTTTTCCTGAAACACCAATACCCCCGCCATTATCTATTCGGGTc                                                                                                                            <  1:21536/148‑1 (MQ=255)
                      cccGCCGTTTTTGATATTTTGCGTTCCGCTGTTGATATGGGTGCTTGTGGTTATGCCATAATTATTAATATTCTGTGTCCCCCCATTAATTATGGTATTTGTCGCGTTTCCTGAAACACCCATACCCCCGCCATTATCTATTCGGGTc                                                                                                                            <  1:794184/148‑1 (MQ=255)
                         gccgcTTTTGATATTTTGCGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATc                                                                                                                       >  2:442266/1‑150 (MQ=255)
                          ccgtTTTTGATATTTTGCGTTCCGCTGTTGATATTGGTGCTTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCTTTTCCTGAAACATCCATACCCCCGCCATTATCTATTCGGGTCGCATCa                                                                                                                      <  2:704371/150‑1 (MQ=255)
                              ttttTATTTTTGGCTTTCCGTTTTTGATTTTGGCCCTTGTGGCTTTCCCTTAATTTTTAATTTTCTGTGCCCCCCAATTATTTTTGGTTTTTGTCGCTTTCCCTGCAACACCTACACCCCCGCCATTATCTATTCGGGTCGCATCAGCtt                                                                                                                  <  1:607243/150‑1 (MQ=255)
                               tttGATATTTTGCGTTCCGCTGTTGATTTTGGTGCCGGTGGCTATGCCATAATTATTAATTTTCTGTGTGCCCCAATTAATTATGGTTTTTGTCGCTTTCCCGGAAACATCCAAACCCCCGCCATTATCTATTCGGGTCGCATCAGCttt                                                                                                                 <  2:324193/150‑1 (MQ=255)
                               tttGATATTTTGCGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCttt                                                                                                                 >  1:524512/1‑150 (MQ=255)
                                    ttttttGCGTTCCGTTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTCCCACCATTAATTATGTTTTTTGTCGCTTTTCCTCCAACATCCAAACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGc                                                                                                              <  2:702311/146‑1 (MQ=255)
                                    tatTTTGCGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCAtt                                                                                                           >  2:528890/1‑151 (MQ=255)
                                      ttttGCGTTCCGTTGTTGATATTGGTGCCTGTGGCTATCCCATAATTATAAATTTTCTGTGTGCCACCATTAATTATGGTTTTTGTCGCGTTTCCTGCAACACCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTg                                                                                                          <  2:89095/150‑1 (MQ=255)
                                      ttttGCGTTCCGCTTTTGATTTTGGTCCCTGTGGCTATGCCATAATTATTAATATTCTGTGTCCCCCCATTAATTTTGGTTTTTGTCGCGTTTCCTGCAACATCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTg                                                                                                          <  1:463662/150‑1 (MQ=255)
                                           cGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTTTTTGTCGCGTTTCCTGCAACATCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTa                                                                                                      <  2:132231/149‑1 (MQ=255)
                                            tttCCGTTGTTGATTTTGGTGTCTGGGTCTATGCCATATTTATTAATTTTCTGTGTGCCCCCTTAATTTATGTTTTTTGTCGCTTTTCCTGCACCATCAATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTaa                                                                                                     <  1:304133/148‑1 (MQ=255)
                                               ccGCTTTTAATATGGGTGCCTGTGGTTAGGCAATAATTATAAATTTTTTGTGGCCCCCCATTAATTATGTTTTTTGTCGCGTTTCCTCCAACCCCAATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTg                                                                                                <  1:948081/151‑1 (MQ=255)
                                                             tgTGCCCGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCttt                                                                                   >  1:929526/2‑150 (MQ=255)
                                                              gTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTAt                                                                                 >  2:146846/1‑151 (MQ=255)
                                                                 ctttttgCTATTCCTTAATTTTTAATTTTCTATGCCCCCCATTTAATTATGTTTTTTGTCGCGTTTCCTGCACCATCCTTGACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCt                                                                                     <  1:674741/138‑1 (MQ=255)
                                                                 cttgtgGCTATGCCATAATTATTAATATTCTGTGTCCCACCATTAATTATGGTTTTTGTCGCTTTTCCTGCAACATCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATc                                                                                <  2:744117/147‑1 (MQ=255)
                                                                      ggttATGCAATATTTATTAATATTCTGTGTCCCACCATTAATTATGTTTTTTGTCGCTTTTCCTGCAACATCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTta                                                                            <  1:1021570/145‑1 (MQ=255)
                                                                               atatTTATTAATTTTCTGTGTGCCACCATTAATTATGGTATTTGTCGCTTTCCCTGCAACACCCAAAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTg                                                                <  2:986434/151‑1 (MQ=255)
                                                                               aTAATTATTAATTTTCTGTGCCCCACCATTAATTATGTTTTTTGTCGCTTTTCCTGCAACATCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTcg                                                                  <  1:665123/149‑2 (MQ=255)
                                                                                        aaTTTTCTGTGTCCCACCATTAATTATGTTTTTTGTCGCTTTTCCTGCAACACCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGaa                                                       <  1:483116/151‑1 (MQ=255)
                                                                                        aaTATCCTGTGTCCCCCCTTTATTTTTGGTTTTTGTCGCTTTTCCTCCACCATCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGttttc                                                           <  1:184999/147‑3 (MQ=255)
                                                                                           atTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAAcc                                                     >  2:81257/1‑150 (MQ=255)
                                                                                           atTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAAcc                                                     >  2:558240/1‑150 (MQ=255)
                                                                                           atTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAAcc                                                     >  1:366722/1‑150 (MQ=255)
                                                                                                      caccaTTAATTATGGTTTTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGtt                                            <  1:276698/148‑1 (MQ=255)
                                                                                                           ttaattaTGGTTTTTGTCGCTTTTCCTGCAACATCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGACGAACCATATGCGTTTATGt                                       <  2:599654/148‑1 (MQ=255)
                                                                                                            taattgTGTTATTTGGCGCGTTTCCCGCAACACCCAACCCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTAt                                         <  1:870914/145‑1 (MQ=255)
                                                                                                             aattaTGTTTTTTGTCTTTTTCCCTGCAACATCCTAACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTaaa                                  <  1:913674/151‑1 (MQ=255)
                                                                                                                  tgttttttGTCGCTTTTCCTGAAACACCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATg                                <  1:465565/143‑1 (MQ=255)
                                                                                                                  tGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGacc                             >  1:264/1‑151 (MQ=255)
                                                                                                                       tttGTCGCTTTTCCTGCAACACCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGacca                            <  2:524695/147‑1 (MQ=255)
                                                                                                                         tGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGttt                      >  2:580295/1‑151 (MQ=255)
                                                                                                                           tCGCTTTTCCTGCAACATCCAAAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGttt                      <  2:226507/149‑1 (MQ=255)
                                                                                                                            cgttttccccgaaaCATCCATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTc                    <  1:92671/138‑1 (MQ=255)
                                                                                                                                 ttCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTCCAGCa               >  1:214698/1‑150 (MQ=255)
                                                                                                                                        aaCACCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACAtt        <  1:847025/150‑1 (MQ=255)
                                                                                                                                          cATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACATTGTc     >  1:546769/1‑151 (MQ=255)
                                                                                                                                          cATCAATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACATTg       <  1:433383/149‑1 (MQ=255)
                                                                                                                                             ccATACCCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACATTGTCt    <  1:886693/149‑1 (MQ=255)
                                                                                                                                             ccATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACATTGTCTGc  >  1:985103/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATATAATTGTTGTGTCAGCTTTCCCGCCGCTTTTGATATTTTGCGTTCCGCTGTTGATATTGGTGCCTGTGGCTATGCCATAATTATTAATATTCTGTGTGCCACCATTAATTATGGTATTTGTCGCGTTTCCTGCAACATCCATAACCCCGCCATTATCTATTCGGGTCGCATCAGCTTTAGCATTGGTTAAAACTGACATTGTTCCTTTATCTTTAATAATCGTCTTGTTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACATTGTCTGC  >  NC_000913/2781499‑2781790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑