Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 300,721 | 0 | G | T | 42.1% | 61.3 / 16.7 | 38 | Q72K (CAA→AAA) | paoC | PaoABC aldehyde oxidoreductase, Moco‑containing subunit |
Reads supporting (aligned to +/- strand): ref base G (5/17); new base T (16/0); total (21/17) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.04e-06 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGT > NC_000913/300586‑300858 | ccACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATGACAGCCCGTACGCCCGGCGCTTTTTTCgcgg > 2:179504/1‑141 (MQ=255) cgcAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAAAGAAAGCCCGTACGCCCCGCGCCTTTTTCGCGGCGTcccggtcaaaggggggg > 2:465434/1‑136 (MQ=255) gcAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTg < 1:419625/150‑1 (MQ=255) aaTGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATGAAAGCCAGTACCCCCCGCGCCTTTTTTGCGGCCGCCGGGTC‑AAggggggggggg > 2:726010/1‑146 (MQ=255) aTGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATGACAACCCCTAATCCCCGCGCTTTTTTTGCGGCGGCCGTATC‑AAGGGGGGTggggg > 1:152072/1‑150 (MQ=255) ccTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATGACAGCCCGTACACCCGGCGCTTTTTTTGGGGGGGCCGGATT‑AAgggggggggggggggc > 2:772253/1‑141 (MQ=255) gatgatAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCttt < 2:172470/151‑1 (MQ=255) gatgatAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATTAAAGCCAGTACGCCCCGCGCTTTTTTCGCGGCGTCCGTATC‑CAAGGCggggggggggccctt > 2:344806/1‑138 (MQ=255) agatAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAAAGAAAAACAGTACCCCCGGGGCCTTTTTTGCGGCGGCCCGTTC‑AAAGGGGGGGAgggggccctttg > 2:598567/2‑141 (MQ=255) cTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATTAAAGCCCATACGCCCCGCGCTTTTTTCGCGGGGGCCGTATC‑AAGGGGGGGGGGGCGGCCTTTTGCAATgg > 2:753099/1‑149 (MQ=255) cAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTTATGAAAACCACTACGCCCCGCGCTTTTTTCGCGGCGCCCCCATC‑AAGGGGGGGGGGGCGTCCttttggaacgg > 2:150075/1‑139 (MQ=255) cAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCg < 2:812219/149‑1 (MQ=255) cAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCg < 1:307429/149‑1 (MQ=255) cAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAAAGACAACCCGGAAGCCCCGCGCTTTTTTCGCGGCGGCCGGATCAAAGGGGGGGGGGGGGCCCTTTGGCatggggg > 2:999336/1‑144 (MQ=255) aTAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAAc < 1:839301/151‑1 (MQ=255) aTAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGGACGCCCGGCGCTTTTTTTGCGGCGGCCGGATC‑AAGGGCGGGGGGGCGGCCTTTGGCGAAGGCggga > 2:146899/1‑148 (MQ=255) cgccTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGAtata < 2:476200/151‑1 (MQ=255) ccTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑ggcg < 2:106857/151‑1 (MQ=255) ggCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑Ggcggcg < 1:140086/151‑1 (MQ=255) ttGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGct < 2:318471/150‑1 (MQ=255) cTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCCGCGCTTTTTTCGCGGGGGCCGGATC‑AAAGGCGGGGAGGCGGCCTTTGGCAATGGCGGGAACGCCGATATAACCATAGGGGGGGGTTGGGGGctctt > 2:713620/1‑144 (MQ=255) tttGCCGAGTGCCCCGGCGTTACTGGCGGTAATGAAAGCCAGTAAGCCCGGCGCCTTTTTCGCGGGGGCCGGATC‑AAAGGGGGGGGGGGGGCCTTTGGGAATGGGGGGAACGGCGATATAGGCCTA‑GGGGGGGGTgggggggtttttat > 2:296711/1‑140 (MQ=255) ttGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTAAGCCCGGCGCCTTTTGCGCGGCGTCCGTATC‑AAGGGCGGGGGGGCGGCCTTTTGCAATGGCGGGACCGACGATATAGCCATA‑GGCGGCGTTGGGGGGTTTTTCATGcc > 2:773890/1‑151 (MQ=255) tGCCGAGTGCCCCGGCGTTACTGGCGGTAAATACAACCCCCACGCCCGGCGCCTTTTTCGCGGGGTCCGGTTC‑CAGGGGGGTGGGGGGGCCTCTTGCAAAGGGGGGACCGACGCAATAACCCTA‑AGCGGGGGTgggggggtttt > 1:1010131/1‑138 (MQ=255) ccGAGTGCCCCGGCGTTACTGGCGGTAAAGACAACCCGGACCCCCCGCGCTTTTTTCGCGGCGGCCGGATC‑AAGGGGGGGGGGGGGTCCTTTGGCAAAGGCGGGACCGCCGGAATAGCCCTA‑GGCGGCGTTGGGGGGGTCTTTATGCCCtt > 1:1011571/1‑151 (MQ=255) cGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTAAGCCCCGCGCTTTTTTCGCGGCGGCCGGATC‑AAGGGGGGGGAGGCGGCCTTTTGGCATGGCGGGAACGACGGGATAACCATA‑GGGGGGGGTGGGGGGGTCTTTATTCCCtt > 1:666868/1‑150 (MQ=255) gCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAgg < 1:676238/151‑1 (MQ=255) ccccGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAgg < 1:365051/150‑1 (MQ=255) ccccGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAgg < 2:835058/150‑1 (MQ=255) ccGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGt < 2:43351/151‑1 (MQ=255) cGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGcg < 2:357044/151‑1 (MQ=255) ggCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGcgtgccg < 1:976213/149‑1 (MQ=255) cGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCgtgccgtgcc > 2:1015088/1‑151 (MQ=255) gTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGGAGCGTGCCGTGCCgg > 2:735865/1‑151 (MQ=255) tAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCgg > 1:444341/1‑150 (MQ=255) aTGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAg < 1:636879/151‑1 (MQ=255) gCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCg > 1:12011/1‑151 (MQ=255) cccGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGt < 2:783652/151‑1 (MQ=255) | CCACTACCAGCGCAATGGCCTGATGATAGTGCTCAATAGTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATC‑AAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATA‑GGCGGCGTTGGGGGCTTCTTCATGCCATTCGTAGGCGTAGCGTGCCGTGCCGGTAGTTTTCAGCGGTCCGT > NC_000913/300586‑300858 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |