Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,042,993 | 0 | C | T | 42.3% | 60.7 / 19.5 | 52 | F193F (TTC→TTT) | srkA | Cpx stress response Thr/Ser protein kinase; MazF antagonist protein |
Reads supporting (aligned to +/- strand): ref base C (9/21); new base T (22/0); total (31/21) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.80e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT > NC_000913/4042846‑4043142 | cccGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTAcc > 2:640016/1‑151 (MQ=255) ccGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTAcc > 1:925380/1‑150 (MQ=255) cGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCg > 1:445780/1‑150 (MQ=255) taCCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAGTTTACCGtt > 1:400032/2‑151 (MQ=255) aCCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCgct > 1:503457/1‑148 (MQ=255) ccATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCt < 2:876704/151‑1 (MQ=255) cATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTCt > 1:868328/1‑150 (MQ=255) cATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTCACCGtt > 2:935707/1‑148 (MQ=255) gTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTAACGTTCttggg > 2:936953/1‑145 (MQ=255) tttGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCgg > 1:897859/1‑148 (MQ=255) tttGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTTCGGCt > 2:634509/1‑150 (MQ=255) gAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACAt < 2:57893/151‑1 (MQ=255) tatCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCCACATgggg > 2:299498/1‑146 (MQ=255) aTTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAc < 1:1016245/150‑1 (MQ=255) gCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTTTGTGGGTTCATGGGGACATCCCA‑GCCggg > 1:736499/1‑151 (MQ=255) cGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAat < 2:861303/150‑1 (MQ=255) aaGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGGTCTGCGGCTACATGGAGAATGCCAC‑GCCGGGAAAAtt > 2:584048/1‑150 (MQ=255) aaGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATtc < 2:114573/151‑1 (MQ=255) tGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGGCACATGGAGACTGCCAC‑GCCGGGGAAATTctct > 2:970731/1‑150 (MQ=255) gTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTTCCGCTCTTCGGCTACATGGAGAATTCCCA‑GCCCGGAATATTTTCTgg > 2:311904/1‑151 (MQ=255) tGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTTTGCGGCTACATGGGGACTGCCCC‑GCCGGGAATATTctct > 2:523686/1‑146 (MQ=255) aGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGGATATTCTCTGgcg > 2:419351/1‑148 (MQ=255) gACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCCACCTGGAGACTGCCCC‑CCCGGGGAAATTCTCTGGcgcggt > 1:917570/1‑148 (MQ=255) gCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGGATATTTTCTTGCGCGGTgg > 2:92181/1‑149 (MQ=255) acacTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCaa < 2:976103/151‑1 (MQ=255) tGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTTACCGTTCTGGGGCTACCTGGGGACTGCCCC‑GCCGGGGATATTTTCTTGGGCGGTGGTCCCAtgtt > 1:365360/1‑151 (MQ=255) tACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGGGACTGCCAC‑GCCGGGGATATTCTCTGGCGCGATGGTCCAAtgtttgt > 2:508603/1‑151 (MQ=255) ccGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCt < 1:18124/151‑1 (MQ=255) gggTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCCC‑GCCGGGGATATTTTCTGGCGCGGTGGTCCAATGTTTGTTGGTCTg > 1:405883/1‑150 (MQ=255) tGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGGTTTGCGGCTACATTGAGACTGCCCC‑GCCGGGGATATTTTCTGGCGCGATGGGCCAATGTTTGTTGATCTGgatg > 2:679859/1‑150 (MQ=255) gCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCCC‑GCCGGGGATATTTTCTGGCGCGGTGGGCCAAAGGTTGTTGATCTGggtg > 1:827957/1‑145 (MQ=255) cATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTaa < 1:935430/151‑1 (MQ=255) cATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTaa < 1:508423/151‑1 (MQ=255) ccTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATgg < 2:579470/150‑1 (MQ=255) gAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTTGGGCGGTGGTCCAATGTTTGTTGGTCTGGGTGATGCACGTAATGGTcc > 2:706528/1‑150 (MQ=255) gAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCa < 1:522237/151‑1 (MQ=255) aaaGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGGATATTTTCTGGCGCGATGGTCCCATGTTTGTTGATCTGGATGATGCACGTAATGGTcc > 2:49667/1‑149 (MQ=255) cGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGGTCTGCGGCTACCTGGAGACTGCCAC‑GCCGGGGAAATTTTCTTGCGCGATGGTCCCATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGcc > 1:128457/1‑149 (MQ=255) gACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑CCCGGGGATATTCTCTGGCGCGGTGGTCCAATGTTTGTTGATTTGGGTGATGCACGGAATGGGCCAGCCGtt > 2:282178/1‑151 (MQ=255) aCAGATGGGCGGATTGCCGCCGTTACAGCACCCTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGtt < 1:915132/150‑1 (MQ=255) cAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCa < 2:500121/151‑1 (MQ=255) cAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGTTCTGGGGCCACATGGAGACTGCCAA‑GCCGGGGATATTCTCTTGCGCGAAGGTCCAATGTTTGTTGGTATGGATGATGCACGTAATGGTCCAGCCGtt > 1:996407/1‑149 (MQ=255) aGATGAGCTGCTTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCCCCCTTCTGCGGCTACCTGTAGGCCCCCACTGCCGGG‑ATATTTTCTCGCGCGCGGGTTCAATGTTTTTTGATCTTGATGATGTGCGGAATAGTCccgccggt > 1:183496/1‑146 (MQ=255) gAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGAt < 1:448781/150‑1 (MQ=255) caaaTTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGAtt < 2:697731/145‑1 (MQ=255) gccgTTACAGCACACTGGCGGGAAGAATTTACCGTTCTGCGGCTACATGGAGACTGCCCC‑GCCGGGAATATTTTCTGGCGCGATGGGCCCAAGTTTGTTGATCTGGGTGATGCACGTAATTGTCCAGCCGTTCAGGGTTTTTGGGTGtt > 1:917822/1‑149 (MQ=255) ccgTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCt < 2:886489/151‑1 (MQ=255) aCAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATg < 1:205277/151‑1 (MQ=255) aGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGc < 2:1005135/151‑1 (MQ=255) cTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGATGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATaa < 1:634306/150‑1 (MQ=255) tCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGa < 2:464402/150‑1 (MQ=255) tCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGa < 1:359058/150‑1 (MQ=255) cACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAt < 2:254731/150‑1 (MQ=255) | CCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAC‑GCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGAT > NC_000913/4042846‑4043142 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |