Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,2880TG45.3% 73.1 / 17.8 55V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (7/22);  new base G (24/0);  total (33/22)
Fisher's exact test for biased strand distribution p-value = 3.50e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354145‑354437
                                                                                                                                                |                                                                                                                                                       
cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGAt                                                                                                                                                  <  1:787205/151‑1 (MQ=255)
 aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGaaaatg                                                                                                                                                  >  1:585967/1‑148 (MQ=255)
 aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATg                                                                                                                                                 >  2:790642/1‑151 (MQ=255)
    cGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcgc                                                                                                                                              <  1:499460/151‑1 (MQ=255)
     gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATgcg                                                                                                                                               >  2:983661/1‑149 (MQ=255)
        aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCTgg                                                                                                                                          >  1:624961/1‑149 (MQ=255)
          gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAg                                                                                                                                       <  1:768866/151‑1 (MQ=255)
          gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTTGGGGGGAAGAAgcgccgga                                                                                                                                          >  1:598037/1‑145 (MQ=255)
              aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGAGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAggg                                                                                                                                     >  2:998939/1‑149 (MQ=255)
                  ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAgg                                                                                                                                 >  2:179505/1‑149 (MQ=255)
                   tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtg                                                                                                                               <  2:627021/150‑1 (MQ=255)
                      cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcgg                                                                                                                           <  2:999065/151‑1 (MQ=255)
                      cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcgg                                                                                                                           <  1:587929/151‑1 (MQ=255)
                      cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcg                                                                                                                            <  2:100581/150‑1 (MQ=255)
                        cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggt                                                                                                                          <  2:203265/150‑1 (MQ=255)
                           tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGGATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAATGGCTGTAAGgggcgggggcgg                                                                                                                           >  1:547501/1‑146 (MQ=255)
                              tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGtt                                                                                                                    <  2:677354/150‑1 (MQ=255)
                              tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGt                                                                                                                     <  2:482802/149‑1 (MQ=255)
                              tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCTCGAAGGGGcgggggcggggg                                                                                                                        >  1:752968/1‑146 (MQ=255)
                                    gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCAtt                                                                                                            <  1:768425/151‑1 (MQ=255)
                                      gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGGGGGGGTTGTCAtttc                                                                                                          >  2:859845/1‑149 (MQ=255)
                                        aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGa                                                                                                        <  1:747101/151‑1 (MQ=255)
                                        aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCg                                                                                                         >  1:125532/1‑150 (MQ=255)
                                          tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGaa                                                                                                       >  2:713631/1‑150 (MQ=255)
                                          tGACGATGTAATTAACGTTCCCGGGCATCGGCTGGGTACCCGCGGGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGCGGGAGCTGCGCTGTCAGGGCCCCTGGCGGGGGGGATTGTctatc                                                                                                           >  1:158008/1‑142 (MQ=255)
                                           gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaaa                                                                                                      <  2:235085/150‑1 (MQ=255)
                                               aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGGTGGGCTGGAAGGGGCAGGGGGGGGGGCGGTTGTCATTTCGtaa                                                                                                      >  2:123710/1‑146 (MQ=255)
                                                      tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTTCGAAaga                                                                                                    >  2:44384/1‑141 (MQ=255)
                                                           ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTGATTTCGAAAGAGAGGGGCAGGCTgg                                                                                      >  2:279041/1‑150 (MQ=255)
                                                             gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                    <  2:632039/150‑1 (MQ=255)
                                                               cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                                 <  2:851373/151‑1 (MQ=255)
                                                                    aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGa                                                                            <  2:645272/151‑1 (MQ=255)
                                                                       ggCTGGGGAAGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGAGAAAGAAGGGCTGAAAGGGGAGGGGGCGGTGGGGGTTGTCATTCCGAAAAAGAGCGACAGTCCTGAAAATCggg                                                                             >  2:822340/1‑145 (MQ=255)
                                                                           ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGGGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                     >  2:985266/1‑151 (MQ=255)
                                                                                 gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                                >  2:619521/1‑150 (MQ=255)
                                                                                  cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                               <  2:336497/150‑1 (MQ=255)
                                                                                    cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCaa                                                            <  1:405810/151‑1 (MQ=255)
                                                                                     gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGTGGAAGGTGCGCCGAAAGGGGAGGGGGCGGGGGCGGGTGTTCTTTCCGGGGAGAAGGGCAGTTTGGGAGATCGTGGTGAGGCGCCCCCGc                                                              >  1:16580/1‑148 (MQ=255)
                                                                                       gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAAAGCGCCGGAAGGGCCGGGGGCGGGGGCGGTTTTCATTCCGAAAAAGAGCGACAGGCTGGAAGATCGTGATGTGGCGGACCCGcca                                                            >  1:443176/1‑146 (MQ=255)
                                                                                        aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTTATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTc                                                                >  2:348445/1‑143 (MQ=255)
                                                                                        aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTTGGGGGAAAGAAGCGCGGTAAGGGCAGGGGGGGGGTGCGGTTGGCATTCCGCGAGTGGGCGACACACTGGGAGAATGTGATGGGGCGCACTCGccaag                                                          >  2:515151/1‑145 (MQ=255)
                                                                                         ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTTCGGAAGAGGGGGGCAGGCTGGGAGATCGCGATGGGGCGCGCTCGCCagag                                                         >  1:461586/1‑149 (MQ=255)
                                                                                                  gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGGGCTGGAAAGGCAGGGGGGGGGGGGGGTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGGGCACTCGCCAGAGAAGGCGAtt                                                >  1:529186/1‑149 (MQ=255)
                                                                                                       tcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGCGAtt                                                >  2:400977/1‑144 (MQ=255)
                                                                                                            ccATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCCGGAAGGGGAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGGCAGTCTGGGAGAACGGGATGTGGCGCACTCGCCAGAGAAGGGGATTATGGCGCtgggg                                    >  1:338197/1‑149 (MQ=255)
                                                                                                            ccATCCGGGCGTTGCCGAAGTGGCGGCGGTTGGGGGGGAAGTGGCGCTCACAGGGCGCGCTGCGGTGGGGTTTGGCATTTCCAAAGAGAGAGACCGGCTGGGAGGGTGTGATGTGGGGGACTCGCAAGAAAAGGCGGTTaatg                                            >  1:1041331/1‑140 (MQ=255)
                                                                                                                  gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGc                              <  2:343051/151‑1 (MQ=255)
                                                                                                                        tGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAt                         <  2:925947/150‑1 (MQ=255)
                                                                                                                                tGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGCGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt                >  2:183281/1‑151 (MQ=255)
                                                                                                                                 ggCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt                <  1:903392/150‑1 (MQ=255)
                                                                                                                                     gtggtTGGGGTGAAAGATGCGCTGAAAGGGCGGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc           >  1:25832/1‑151 (MQ=255)
                                                                                                                                        ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc         >  2:188864/1‑151 (MQ=255)
                                                                                                                                        ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg          <  1:112381/150‑1 (MQ=255)
                                                                                                                                           tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg      >  2:1005991/1‑151 (MQ=255)
                                                                                                                                               gTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  >  1:645771/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                       
CATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354145‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: