Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,356,297 A→T W272R (TGG→AGG)  sapA ← antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,356,2970AT95.9% 169.2 / NA 49W272R (TGG→AGG) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (22/25);  minor base C (2/0);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCG  >  NC_000913/1356156‑1356441
                                                                                                                                             |                                                                                                                                                
ccAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAAc                                                                                                                                         <  2:234306/151‑1 (MQ=255)
      cATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGGGGGGTTAAAATCCACAAAGGGGGCGGTCATCCCCAGGCGCAGAGGTTAAAGCAAGAGCGGGGCGGCACGCAAAAAAGGTAAGTGGGTGGCCGCAGGGCCCGCCACAACGt                                                                                                                                       >  1:435955/1‑147 (MQ=255)
       aTGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCAt                                                                                                                                  <  1:196816/151‑1 (MQ=255)
              aCAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCgg                                                                                                                           <  1:763461/151‑1 (MQ=255)
                   gggATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAgg                                                                                                                      <  2:969151/151‑1 (MQ=255)
                     gATTATTTAGCGGCGGTTTGGCGGGGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAAAGCAAACGCGGGGCGTCACGCCAAAAGGATAGCTGGGTGGCAGCAGGCCCGGGCAGAACGGCGCATTCCCCgggccgg                                                                                                                      >  2:766101/1‑144 (MQ=255)
                        tattTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGtt                                                                                                                  <  2:877114/150‑1 (MQ=255)
                         attTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGttt                                                                                                                 <  1:828086/150‑1 (MQ=255)
                         attTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTc                                                                                                                <  2:713897/151‑1 (MQ=255)
                               cggcggTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGAc                                                                                                             >  2:245605/1‑148 (MQ=255)
                                      ttGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGtt                                                                                                                  <  2:482719/136‑1 (MQ=255)
                                      ttGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGtt                                                                                                                  >  1:482551/1‑136 (MQ=255)
                                             tgtTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgccgcc                                                                                            >  1:90952/1‑151 (MQ=255)
                                             tgtTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgccgc                                                                                             <  1:325333/150‑1 (MQ=255)
                                                     gCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCt                                                                                     <  1:463348/150‑1 (MQ=255)
                                                          aTAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATcc                                                                               >  1:118441/1‑151 (MQ=255)
                                                           tAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCAGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATcc                                                                               >  2:141042/1‑150 (MQ=255)
                                                            aGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATccac                                                                             <  2:460432/151‑1 (MQ=255)
                                                                gACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACt                                                                         >  2:795185/1‑151 (MQ=255)
                                                                gACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACt                                                                         >  1:676823/1‑151 (MQ=255)
                                                                      cATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGc                                                                   >  1:210031/1‑151 (MQ=255)
                                                                         cccAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCTCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGc                                                                >  2:772179/1‑151 (MQ=255)
                                                                          ccAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGc                                                                >  2:1041832/1‑150 (MQ=255)
                                                                          ccAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCa                                                               <  1:483957/151‑1 (MQ=255)
                                                                            aGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCAtt                                                             >  2:672780/1‑151 (MQ=255)
                                                                             ggACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTa                                                            <  2:29173/151‑1 (MQ=255)
                                                                                  cAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACgg                                                        <  2:449935/150‑1 (MQ=255)
                                                                                     cGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTa                                                    <  2:207177/151‑1 (MQ=255)
                                                                                         aaaCGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACcgc                                                >  1:758552/1‑151 (MQ=255)
                                                                                             gCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGcc                                             >  2:436731/1‑150 (MQ=255)
                                                                                             gCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCa                                            <  1:423051/151‑1 (MQ=255)
                                                                                               aaGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGcc                                             >  2:998744/1‑148 (MQ=255)
                                                                                               aaGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGa                                          <  1:519433/151‑1 (MQ=255)
                                                                                                   gcgGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGtc                                      >  1:759955/1‑151 (MQ=255)
                                                                                                   gcgGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGtc                                      <  1:957732/151‑1 (MQ=255)
                                                                                                             aCGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGt                            >  2:427540/1‑151 (MQ=255)
                                                                                                                     tGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCg                    >  2:472158/1‑151 (MQ=255)
                                                                                                                          aGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATa                <  2:192007/150‑1 (MQ=255)
                                                                                                                           gctggctgGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATaaa              <  2:853195/151‑1 (MQ=255)
                                                                                                                           gctggctgGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATaa               >  2:331595/1‑150 (MQ=255)
                                                                                                                             tggctggcAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAAtt            >  2:273904/1‑151 (MQ=255)
                                                                                                                              ggctggcAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAAtt            <  2:442637/150‑1 (MQ=255)
                                                                                                                              ggctggcAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAAt             <  1:791225/149‑1 (MQ=255)
                                                                                                                              ggctggcAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTg           <  1:199585/151‑1 (MQ=255)
                                                                                                                                 tggcAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGccc        >  1:61672/1‑151 (MQ=255)
                                                                                                                                 tggcAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGccc        >  1:620164/1‑151 (MQ=255)
                                                                                                                                  ggcAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGccc        <  1:774685/150‑1 (MQ=255)
                                                                                                                                     agcagGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCGCTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGgcg    <  1:380649/151‑1 (MQ=255)
                                                                                                                                     agcagGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGgcg    >  1:261347/1‑151 (MQ=255)
                                                                                                                                       caggcctggcCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGcgcg  <  2:848753/151‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
CCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGCG  >  NC_000913/1356156‑1356441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑