Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,673	A→C	F139V (TTC→GTC)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,673	0	A	C	100.0%	289.3 / NA	73	F139V (TTC→GTC)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (43/30);  total (43/30)

GCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAT  >  NC_000913/3400526‑3400820                                                                                                                                                    |                                                                                                                                                    gcACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGgacgac                                                                                                                                                  <  2:29472/151‑1 (MQ=255)   acaGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACtt                                                                                                                                                <  2:955026/151‑1 (MQ=255)    caGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTc                                                                                                                                               <  1:278590/151‑1 (MQ=255)     agaagaGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCa                                                                                                                                              <  2:300635/151‑1 (MQ=255)         gaggagTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACggg                                                                                                                                          >  1:882492/1‑151 (MQ=255)           ggagTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGc                                                                                                                                         >  2:608483/1‑150 (MQ=255)              gTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCa                                                                                                                                     <  1:685931/151‑1 (MQ=255)                   ccACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCCCCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAgcgc                                                                                                                                 <  2:1037199/150‑1 (MQ=255)                   ccACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGcc                                                                                                                                >  2:285884/1‑151 (MQ=255)                   ccACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGcc                                                                                                                                >  2:822638/1‑151 (MQ=255)                       acCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTg                                                                                                                             >  2:688133/1‑150 (MQ=255)                       acCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTg                                                                                                                             <  1:687918/150‑1 (MQ=255)                         cGTTCAAGGAGATAAGAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTgcgc                                                                                                                          >  1:191717/1‑151 (MQ=255)                         cGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTgcg                                                                                                                           >  2:718428/1‑150 (MQ=255)                            tCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCgg                                                                                                                        >  2:65816/1‑150 (MQ=255)                            tCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCgg                                                                                                                        >  1:373401/1‑150 (MQ=255)                            tCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACAATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCgg                                                                                                                        >  1:153458/1‑150 (MQ=255)                                  agaTAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACg                                                                                                                 >  1:615298/1‑151 (MQ=255)                                   gaTAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACg                                                                                                                 >  1:387527/1‑150 (MQ=255)                                   gaTAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGa                                                                                                                <  2:257847/151‑1 (MQ=255)                                     tAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAAt                                                                                                              >  1:521742/1‑151 (MQ=255)                                     tAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAAt                                                                                                              >  1:929549/1‑151 (MQ=255)                                        cAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgc                                                                                                           <  1:815240/151‑1 (MQ=255)                                          gCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCCCCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcgc                                                                                                         <  2:43656/151‑1 (MQ=255)                                                 cAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGtt                                                                                                   <  1:15647/150‑1 (MQ=255)                                                 cAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTc                                                                                                  >  1:705472/1‑151 (MQ=255)                                                  aGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTc                                                                                                  >  1:395246/1‑150 (MQ=255)                                                   gTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTc                                                                                                  >  1:198342/1‑149 (MQ=255)                                                     ggTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAAcc                                                                                              <  1:33931/151‑1 (MQ=255)                                                           accaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTc                                                                                        >  2:947520/1‑151 (MQ=255)                                                              accGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTcgcg                                                                                     <  1:911542/151‑1 (MQ=255)                                                                 gATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGcc                                                                                   >  2:898256/1‑150 (MQ=255)                                                                 gATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGcc                                                                                   >  2:517928/1‑150 (MQ=255)                                                                 gATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCa                                                                                  <  2:281178/151‑1 (MQ=255)                                                                      cAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCg                                                                              <  2:938212/150‑1 (MQ=255)                                                                        accaccATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGc                                                                            >  1:160946/1‑150 (MQ=255)                                                                           accaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcaca                                                                         >  2:660019/1‑150 (MQ=255)                                                                            ccaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcacac                                                                        >  2:499678/1‑150 (MQ=255)                                                                            ccaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcacac                                                                        >  1:834725/1‑150 (MQ=255)                                                                             caTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACaa                                                                      <  1:367691/151‑1 (MQ=255)                                                                              aTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACaa                                                                      >  2:955201/1‑150 (MQ=255)                                                                                aGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAAcc                                                                   >  1:909043/1‑151 (MQ=255)                                                                                   aCCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGa                                                                <  1:622771/151‑1 (MQ=255)                                                                                     cGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAc                                                              <  2:83478/151‑1 (MQ=255)                                                                                       gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcgc                                                            <  2:448909/151‑1 (MQ=255)                                                                                       gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcgc                                                            <  2:25886/151‑1 (MQ=255)                                                                                         cGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcgcgc                                                          >  2:622745/1‑151 (MQ=255)                                                                                          gCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcgcgcg                                                         <  2:615496/151‑1 (MQ=255)                                                                                                gAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTg                                                   >  2:59450/1‑151 (MQ=255)                                                                                                 aaaCCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTgg                                                  >  1:427085/1‑151 (MQ=255)                                                                                                 aaaCCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTgg                                                  <  1:322863/151‑1 (MQ=255)                                                                                                 aaaCCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTgg                                                  >  1:835538/1‑151 (MQ=255)                                                                                                   aCCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGAc                                                <  2:462424/151‑1 (MQ=255)                                                                                                     cGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACTCGCGGGCTTGGACGc                                              >  2:797396/1‑151 (MQ=255)                                                                                                          ggCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATaa                                          >  2:688028/1‑150 (MQ=255)                                                                                                           gCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATaagg                                        <  2:48533/151‑3 (MQ=255)                                                                                                            ccAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGc                                       >  2:623748/1‑151 (MQ=255)                                                                                                              aGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTg                                     <  2:373539/151‑1 (MQ=255)                                                                                                              aGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTg                                     <  1:551021/151‑1 (MQ=255)                                                                                                                cACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGtt                                   >  2:138093/1‑151 (MQ=255)                                                                                                                       tGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCtgtg                             <  2:153522/150‑1 (MQ=255)                                                                                                                        gCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGc                            >  1:491110/1‑150 (MQ=255)                                                                                                                             aGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGt                      >  2:35656/1‑151 (MQ=255)                                                                                                                              gCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGtt                     <  2:882740/151‑1 (MQ=255)                                                                                                                               ccATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGttt                    >  2:676936/1‑151 (MQ=255)                                                                                                                               ccATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGttt                    <  1:59426/151‑1 (MQ=255)                                                                                                                                    ggTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatg                >  2:440670/1‑150 (MQ=255)                                                                                                                                         ttcAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGCTTCACGAATTGCGCGGCGTTCAACCTGGGCCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTg           >  1:346944/1‑150 (MQ=255)                                                                                                                                            aaTCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTgg       >  2:97437/1‑151 (MQ=255)                                                                                                                                            aaTCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTgg       >  2:985773/1‑151 (MQ=255)                                                                                                                                            aaTCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTgg       >  2:805777/1‑151 (MQ=255)                                                                                                                                             aTCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCGCACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTgg       >  1:1035457/1‑150 (MQ=255)                                                                                                                                               cAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAg     <  2:395390/150‑1 (MQ=255)                                                                                                                                                 ggACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAt  <  2:447939/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAT  >  NC_000913/3400526‑3400820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑