Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC44.4% 98.3 / 20.4 63T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (31/4);  new base C (0/28);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 6.43e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGC  >  NC_000913/1376361‑1376653
                                                                                                                                                 |                                                                                                                                                    
ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTc                                                                                                                                                 >  1:322480/1‑150 (MQ=255)
 cgccgATGACCTCGCCGCGTAGCCTGGACGGCGTCCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGc                                                                                                                                               <  2:352054/151‑1 (MQ=255)
   ccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCg                                                                                                                                             <  1:836708/151‑1 (MQ=255)
     gATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGAt                                                                                                                                           >  2:434056/1‑151 (MQ=255)
         aCCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGccc                                                                                                                                       >  1:398200/1‑151 (MQ=255)
           cTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCccgc                                                                                                                                     >  1:187371/1‑151 (MQ=255)
            tCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCTCTATCAGGATCATATGATCAACACCCTCGCCGATGCccgcc                                                                                                                                    >  1:1015067/1‑151 (MQ=255)
              gccgcGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGt                                                                                                                                  >  1:765582/1‑151 (MQ=255)
                    aagcggggACGGCGACCGAAAAAGGGTGAGTGTTCCCCTGCGCGTACGGAAACAGGTCGCCGCGCGTACGGGACCCGTGGTGTATCTCGACCTGTAAACCCGCTATCAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACa                                                                                                                              <  2:706987/143‑1 (MQ=255)
                          ggACGGCGCCCGAAAAATGGTGGGTGATCCCCGGCGCGTACTGGAACGGGCCCCCGCGCGTCCCGGACCGGGGTTGTATCCCAACCCGTAAACCCGCTACCAGGATAAAATGATCACCCCCCCCCCCGATGCCCGCCGTTACATCGTCGaa                                                                                                                      <  1:469677/151‑1 (MQ=255)
                               gcgcCCGAAAAAGGGTGGGTGATCCCCTGCGCGTACGGGAAAGGGTCGCCGCGCGTACCGGAACCGGGGTGTATCTCGACCCTTAAAACCGTTATAGGGATAATATGATCACCCCCCTCGCGGATGCCCGCCGTTACATCGTCGAAAAc                                                                                                                   <  2:830762/149‑1 (MQ=255)
                                   ccGAAAAATGGTGAGTGATCCCCTGCGCGTCCTGGAACAGGTCGCCGGGCGTCCCGGACCGGTGGTGTATCTCGACCCGTAAACCCGCTATCAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCtt                                                                                                             <  2:394200/151‑1 (MQ=255)
                                       aaaaGGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTCCCGGACCCGTGGTGTATCTCGAACCTTAAACCCGTTATCAGGATAATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                                           <  2:348929/149‑1 (MQ=255)
                                       aaaaGGGTGAGTGATCCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTCCCGGACCGGTGGTGTACCTCGACCTTTTAACCCGCTACAAGGATAATATGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                         <  2:1172272/151‑1 (MQ=255)
                                          aTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATg                                                                                                      >  1:1101178/1‑151 (MQ=255)
                                               gagtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                   >  1:651376/1‑149 (MQ=255)
                                               gagtgaTCCCCGGCGCGTACGGAAACAGGTCGCCGCGCGTACCGGAACCGGGGTGTATCTCAAACCTTTAACCCGCTATCAGGATCATATGATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACa                                                                                                  <  2:5260/150‑1 (MQ=255)
                                                  tgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAt                                                                                               >  1:156544/1‑150 (MQ=255)
                                                       cccGGCGCGTACGGGAACAGGCCGCCGCGCGTCCCGGACCCGTGGTGTATCTCGACCCGTTAACCCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCg                                                                                          <  2:322515/150‑1 (MQ=255)
                                                        ccggcgcGTCCTGGAACAGGTCGCCGCGCGTCCCGGAACCGTGGTGTATCTCAACCCGTTAAACCGCTATCAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGc                                                                                        <  2:567163/148‑1 (MQ=255)
                                                             gcgTACGGGAACGGGTCGCCGCGCGTCCCGGACCGGGGGTGTATCTCGACCCTTTAACCCTCTATCAGGATCATATGATAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAtt                                                                                    <  2:1015170/150‑1 (MQ=255)
                                                              cgTACTGGAACGGGTCGCCGCGCGTACCGGAACGGTGGTGTATCCCGACCCTTAAACCCGCTATCAGAATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCg                                                                                       <  2:654745/146‑1 (MQ=255)
                                                                tACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCt                                                                                 >  2:1238229/1‑150 (MQ=255)
                                                                  cggaaaCGGGTCGCCGCGTGTCCCGGAACGGTGGTGTATCTCGACCCGTAAACCCGCTATCAGGATCATAGGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCt                                                                                 <  2:1194239/144‑1 (MQ=255)
                                                                   tGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATc                                                                              >  2:568385/1‑150 (MQ=255)
                                                                    ggagcgggccgccgcGCGTACCGAAACGGTGGTGTATCCGGCACCTTTAAACCGTTAAGAGGATCAAATCATAAACCCCCTCGCGAATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTa                                                                                <  2:1048095/138‑1 (MQ=255)
                                                                    gaaaCAGGCCGCCGCGCGTACCGGACCGGTGGTTTATCCCGAACCTTAAACCCGCTATCAGGATAATATAATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcac                                                                            <  1:305988/149‑1 (MQ=255)
                                                                      aaCGGGTCGCCGCGCGTCCCGGACCCGTGGTGTATCCCGAACCGTTAACCCGCTATCAGGATAATATGACCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTa                                                                                <  2:1057785/145‑1 (MQ=255)
                                                                      aaCGGGTCCCCGCGCTTACGGGACCGGTGGTTTATCCCGACCCTTAAACCCGTTACAAGGATCATATGATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcaca                                                                           <  2:493969/150‑1 (MQ=255)
                                                                      aaCAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACAt                                                                          >  1:843585/1‑151 (MQ=255)
                                                                       aCAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg                                                                         >  2:970690/1‑151 (MQ=255)
                                                                        cAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg                                                                         >  2:1165801/1‑150 (MQ=255)
                                                                        cAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg                                                                         >  2:327182/1‑150 (MQ=255)
                                                                          ggTCGCCGCGCGGCCGGGACCCGTGGTGTATCCCGAACCGTTAACCCGCTATCAGGTTCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGaa                                                                       <  2:328006/150‑1 (MQ=255)
                                                                             cgcggcgatacccGGAACCGTGTTGTACCTCGAACCGTAAACCCGTTATAAGGATAATAGGATCAACACCCTCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAata                                                                     <  2:710177/138‑1 (MQ=255)
                                                                             cgccggGCGCCCGGGACCCGTGTTTTATCCCGACCTGTAAACCCATTATAAGAATCATATGATAACCCCCCGCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAt                                                                                     <  1:183181/133‑1 (MQ=255)
                                                                             cgccgcGCGTCCCGGAACCGTGGTGTATCTCGACCCTTTAACCCGCTATCAGAATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATc                                                                   <  2:765649/151‑1 (MQ=255)
                                                                                  cgcgTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCga                                                                 >  1:571170/1‑148 (MQ=255)
                                                                                  cccgTCCCGAACCCGTGTTTTATCTCGAACGGTTAACCCGCTATCAGTATCATATGATCACCCCCCTCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaag                                                               <  2:579280/148‑1 (MQ=255)
                                                                                    cgcaCCGAACCCGTGGTTTACCCCGACCCGTAACCCCGCTATCAGGTTAAAAAAACCACCCCCCCCGCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaagaa                                                             <  1:231134/147‑1 (MQ=255)
                                                                                     gTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                              >  1:270831/1‑148 (MQ=255)
                                                                                          ggAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAcc                                                      >  2:867833/1‑151 (MQ=255)
                                                                                           gAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCt                                                     >  1:302648/1‑151 (MQ=255)
                                                                                             aCCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTgg                                                   >  1:377421/1‑151 (MQ=255)
                                                                                                  ggttTATCTCAACCCTTTAACCCCTTATAAGAATAATATGATCAACCCCCTCGCCGTTCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCa                                               <  2:675989/150‑1 (MQ=255)
                                                                                                  ggtgTACCCCGACCCTTAAACCCGATAAAAGGATCAAATGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCa                                               <  1:733825/150‑1 (MQ=255)
                                                                                                    tgtATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                            >  2:169275/1‑151 (MQ=255)
                                                                                                          ccaacccGTAAACCCGTTAACAGAATAATATAACCAACCCCCCCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGGGCt                                         <  1:1034133/143‑1 (MQ=255)
                                                                                                             aaCCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                                    >  2:146759/1‑150 (MQ=255)
                                                                                                              aCCCTTAAACCCGCAATCAGGATAATATAACCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcg                                           <  2:618034/143‑1 (MQ=255)
                                                                                                                   tAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATGGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcg                             >  1:808959/1‑151 (MQ=255)
                                                                                                                    aaaCCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                            >  2:1249101/1‑151 (MQ=255)
                                                                                                                        ccGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGAc                         >  1:851593/1‑151 (MQ=255)
                                                                                                                               caggttaatataacaaaCCCCCCCCCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTc                   <  2:398239/135‑1 (MQ=255)
                                                                                                                                agtaTCATATGACCAACCCCCTCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGAcc                        <  2:651434/141‑1 (MQ=255)
                                                                                                                                 ggATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGt                >  1:319423/1‑151 (MQ=255)
                                                                                                                                  gATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTc               >  1:1252923/1‑151 (MQ=255)
                                                                                                                                    tCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt             >  1:557805/1‑151 (MQ=255)
                                                                                                                                       tatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgtg          >  1:160414/1‑151 (MQ=255)
                                                                                                                                       tatGAACACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt             <  2:753723/148‑1 (MQ=255)
                                                                                                                                           aTCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCat       >  2:16782/1‑150 (MQ=255)
                                                                                                                                            tCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCatat     >  1:137483/1‑151 (MQ=255)
                                                                                                                                              aaCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt           <  2:328561/143‑1 (MQ=255)
                                                                                                                                               acacCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGc  >  1:1196771/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGC  >  NC_000913/1376361‑1376653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: