Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,506,0280AC43.2% 139.6 / 11.0 74V18G (GTA→GGA) ydcOBenE family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base A (39/3);  new base C (0/32);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 7.31e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAA  >  NC_000913/1505883‑1506177
                                                                                                                                                   |                                                                                                                                                      
gcgATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGcc                                                                                                                                                   >  2:1112056/1‑151 (MQ=255)
gcgATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGcc                                                                                                                                                   >  1:60718/1‑151 (MQ=255)
   aTACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGcc                                                                                                                                                   >  1:303739/1‑148 (MQ=255)
    tACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGa                                                                                                                                                >  1:522068/1‑150 (MQ=255)
     aCCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGaa                                                                                                                                               <  1:318189/150‑1 (MQ=255)
          aaTGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCg                                                                                                                                        >  2:1162047/1‑151 (MQ=255)
          aaTGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCg                                                                                                                                        >  1:613630/1‑151 (MQ=255)
              tCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGcc                                                                                                                                      >  1:8608/1‑149 (MQ=255)
                    tCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCgt                                                                                                                              >  2:980890/1‑151 (MQ=255)
                      aGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCgtgg                                                                                                                            >  1:1006999/1‑151 (MQ=255)
                          tccTGCCGCCCATTCCCACCCCCGGCCCCGTCACCCACCCAGAGTTTTGGGGGGGGGTGCCCCGGGCGAAAACCGTTGTTTGCAAGTTTATGGCCGCTGAACTGGCTTAGCAAATAAATCCCGCCAGAACCCCCGCCAGTAGCgtgggtg                                                                                                                         <  1:1012929/148‑1 (MQ=255)
                          tACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCgtgggtgg                                                                                                                        >  2:342708/1‑151 (MQ=255)
                             tGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTggagg                                                                                                                     >  2:248879/1‑151 (MQ=255)
                             tGACCCCCTTGGCCACCCCCAGCCCCGTCACCCAGCCAGAGTTTTGTGCGGGGGTGGCCCCGGCGACAATCGCTGCTGCCCAAATTATTGCCGCTAACCGGGCTTAGCAAATTAATCCCCCCAGAACCCCCGCCAGTAGCgtgggtg                                                                                                                         <  1:350530/147‑1 (MQ=255)
                                cccccTTTGCCACCCCCGGCCCCTTCACCCAGCAAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGTTGCTTGCCATTTTTTGCCCGAGGAACGGGCGTAGCAAATAAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTggagga                                                                                                                    <  2:719461/147‑1 (MQ=255)
                                cccccATCGCCACCCCCAGCGCCTTCACCCACCAAGAGTTTGGTGCAGGGGTGCCCCCGCCAACAACCCCTTCTTGCCAATTTATCGCCGCTGAACGGGCTTACCAATTAAATCCCCCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGaa                                                                                                                   <  2:1241959/148‑1 (MQ=255)
                                cGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAAt                                                                                                                  >  2:153380/1‑151 (MQ=255)
                                  ccctttcccgcccccAGCGCCCTCACCCAGCCAGATATTTGTGCAGTGGTGGCCCCGGCGACAATCGCTGCTGGCCAGATTTTTGCCGCTGAACGGGCGTACCAAATAAATCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATa                                                                                                                 <  2:968852/139‑1 (MQ=255)
                                  cccttcgccccccccGGCGCCTCCACCCACCAGGAGTTTTGGGCGGGGGGGGCTCCGGCAAAAACCGCTGCTTGCCATTTTATTGCCGCTGAACGGGGGTAGCAAATAAATCCCCCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAg                                                                                                                <  1:1224370/140‑1 (MQ=255)
                                   ccATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCACTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAAt                                                                                                                  >  2:557671/1‑148 (MQ=255)
                                    cATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGa                                                                                                               >  1:824410/1‑150 (MQ=255)
                                      ttGCCACCCCCAGCGCCGTCACCCGGCAAGAGATTTGTGCGGTGGGGGCCCCGGCAACATTCGCTGCTTGCCAGTTTATTGCCGTGGAACGGGCGTACCAAATAAACCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGaa                                                                                                              <  1:1161926/149‑1 (MQ=255)
                                         ccAGCCCCACCGCCGTCACCCAGCCAGAGTTTTGTGCAGTGGGGCCCCGGGCGAAATTCGCTGTTTCCAAGATTTTTGCCGTTGAACGGGGGTAGCAAATTAATCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACa                                                                                                          <  2:1131844/150‑1 (MQ=255)
                                          cgcccccGGCGCCGTCACCCACCAAGATTTTTGTGCGGGGGGGGCTCGGGCAAAAACCGCTGCTTGCAAGATTTTGGCGGTGGAACGGGGGAAGCAAATAAAACCCCCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGa                                                                                                        <  1:267437/148‑1 (MQ=255)
                                            gCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGAc                                                                                                       >  1:1214195/1‑150 (MQ=255)
                                              cccAGCCCCGCCACCCAGCCAGAATTTTGGGAAGGGGTGCCCCCGGCGACAACCGCTGCTTGCAATTTTATGGCCGAGAAATGGGCGAGGAAAATAAATCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGaaa                                                                                                             <  1:103190/142‑1 (MQ=255)
                                               ccAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCAt                                                                                                   >  1:435707/1‑151 (MQ=255)
                                                      cGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCAcc                                                                                            >  1:294901/1‑151 (MQ=255)
                                                          aTCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGt                                                                                        >  1:167431/1‑151 (MQ=255)
                                                            ccagccagAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGc                                                                                      >  2:1253171/1‑151 (MQ=255)
                                                                caagagTTTTGGGCGGGGGTGCCCCGGGCAACAACCGCTGCTTGCAAATTTTTTGCCGCTGACCGGGGGTAGCAAATAAATCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCg                                                                                         <  1:342673/142‑1 (MQ=255)
                                                                 cagAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtat                                                                                 >  2:1251199/1‑151 (MQ=255)
                                                                      ttttgggctttggtggCTCCGGCGCCAACCGCTGTTGGCCAAATATTGCCCGCTAAACGGGCGTAGACATTAAACCCCGCAAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtata                                                                                <  1:1155008/137‑1 (MQ=255)
                                                                       tttggggggggggggCCCCGGCAAAAATCCCTGCTTCCCAGATTTTTGCCGCTAAACGGGGGTGGCAAATAAAACCCGCAAAAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCAc                                                                                             <  1:1190410/120‑1 (MQ=255)
                                                                       tttGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGt                                                                            >  1:145252/1‑150 (MQ=255)
                                                                       tttGTGCAGTGGTGGCTCCGGCAACAATCGCTGCTTCCAAGTTTTTTGCCGCTAAACGGGCGTAGCAAATTAATCCCGCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAg                                                                               <  1:895792/147‑1 (MQ=255)
                                                                       tttGGGCGGTGGTGCCCCCGGCAAAAATCGCTCCTTGCAAGTTTTTTGCCGCTGACCGGGCGAGGCAAATAAACCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtata                                                                                <  1:1111946/146‑1 (MQ=255)
                                                                        ttGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTAc                                                                          >  1:252238/1‑151 (MQ=255)
                                                                          gtgCGGGGGTGCCCCCGGCGAAAATCGCTGCTTCCAAGATTTTTGCCGCTAAACTGGGGTAGCCAATTAATCCCGCCCGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACa                                                                         <  2:766374/150‑1 (MQ=255)
                                                                          gtgCAGGGGGGGCCCCGGCAAAAACCGCTGCTTGCCAATTTATGGCCGCTGAACGGGCGTAGCAAATAAATCCCCCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTa                                                                           <  2:1067397/148‑1 (MQ=255)
                                                                               gtggtgGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCAcc                                                                   >  1:329202/1‑151 (MQ=255)
                                                                               ggggtggCCCCGGCAACAATCGCTGTTTGCAAGATTTTGGCCGTTGACCGGGGGTAGCAAATAAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCa                                                                     <  1:402119/147‑1 (MQ=255)
                                                                               gggggggCCCCGGCGCAAACCGCTGCTTCCCAGATTATTGCCGCTGACCGGGGGTAGCAAATTAATCCCGCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAgg                                                                       <  2:74840/142‑1 (MQ=255)
                                                                                  gtggCTCGGGCGACAATCGCTGCTTGCCATATTTTGGCCGCTGAAGGGGCGTAGCAAATAAACCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTa                                                                <  1:1168686/151‑1 (MQ=255)
                                                                                       tCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCAtt                                                           >  1:1112987/1‑151 (MQ=255)
                                                                                        ccGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCAttt                                                          >  2:1044077/1‑151 (MQ=255)
                                                                                        ccGGCAAAAACCGCTGTTTCCAAAATTATTGCCGCTGAACGGGCGTAGCAAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCAttt                                                          <  2:32812/151‑1 (MQ=255)
                                                                                            caaaaaTCGCTGTTTGCCAGATTTTTCCCGGAAAACGGGCGTAACCAATAAAAACCGCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGt                                                        <  1:749811/145‑1 (MQ=255)
                                                                                            cGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTcc                                                      >  1:1205673/1‑151 (MQ=255)
                                                                                             gACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCg                                                     >  2:958733/1‑151 (MQ=255)
                                                                                             gACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCg                                                     >  2:843694/1‑151 (MQ=255)
                                                                                                aaTCGCTGCTGGCCAGATTATTGCCGCTAAACGGGCGTAGCAAATTAATCCCGCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGtt                                                   <  2:60721/150‑1 (MQ=255)
                                                                                                aaTCGCTGCTGGCAAGTTTATGGCCGTTGAGCTGGGGTAGCAAATAAAACCCGCCAAAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGtt                                                   <  1:598902/150‑1 (MQ=255)
                                                                                                  tCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTtat                                                 >  1:403175/1‑150 (MQ=255)
                                                                                                     ctgctTGCCAGATTATGGCCGCTGACCGGGGGTAGCAAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTtata                                                <  1:363802/148‑1 (MQ=255)
                                                                                                            ccAGATTATTGCCGCTGAACTGGCGTAGCAAATAAACCCCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGt                                      <  2:1144884/151‑1 (MQ=255)
                                                                                                              aGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTg                                     >  2:378858/1‑150 (MQ=255)
                                                                                                                 tcattGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATa                                  >  1:1122097/3‑150 (MQ=255)
                                                                                                                     tGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACtt                             >  1:1109909/1‑151 (MQ=255)
                                                                                                                             gagctgggcaatcaaaTTAATCCCCCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTc                            <  1:248854/131‑1 (MQ=255)
                                                                                                                              aaCGGGCGTACCAAATTAACCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGtttt                      <  1:501549/150‑1 (MQ=255)
                                                                                                                                  ggCGTACCAAATTAACCCCCCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGc                    <  2:824483/148‑1 (MQ=255)
                                                                                                                                   ggggacccAATAAACCCCGCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCgga                <  1:153367/145‑1 (MQ=255)
                                                                                                                                   gCGTAGCAAATAAATCCCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCgga                <  2:522116/151‑1 (MQ=255)
                                                                                                                                    cGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCgg                 >  1:844846/1‑149 (MQ=255)
                                                                                                                                     gTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCggagg              >  2:837590/1‑151 (MQ=255)
                                                                                                                                      tAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCggagg              >  1:144589/1‑150 (MQ=255)
                                                                                                                                      tAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCggagg              >  1:1017371/1‑150 (MQ=255)
                                                                                                                                       accaaaTTAACCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCggag               <  2:145264/144‑1 (MQ=255)
                                                                                                                                           aaTTAATCCCCCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCgga                <  2:349975/143‑1 (MQ=255)
                                                                                                                                                aaTCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCggag               <  2:1243823/140‑1 (MQ=255)
                                                                                                                                                aaTACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATgg    >  2:260784/1‑151 (MQ=255)
                                                                                                                                                 aTACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATgg    >  2:376641/1‑150 (MQ=255)
                                                                                                                                                 aTACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATgg    >  2:779715/1‑150 (MQ=255)
                                                                                                                                                   aCCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGaa  >  2:143795/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGGAGGAGTCGCATGGAA  >  NC_000913/1505883‑1506177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: