Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	43.3%	78.4 / 40.5	67	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (5/33);  new base T (29/0);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 4.05e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206285‑3206583                                                                                                                                                       |                                                                                                                                                       cAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTc                                                                                                                                                        <  2:816407/151‑1 (MQ=255)   gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                                      <  2:1185790/151‑1 (MQ=255)       aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAc                                                                                                                                                   <  2:961775/150‑1 (MQ=255)        tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACa                                                                                                                                                  >  1:384498/1‑150 (MQ=255)           cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACaaa                                                                                                                                                >  2:1071584/1‑149 (MQ=255)           cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGC‑AAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATa                                                                                                                                              <  1:733145/150‑1 (MQ=255)               aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa                                                                                                                                          <  1:918019/151‑1 (MQ=255)                 aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaaa                                                                                                                                        <  2:7596/151‑1 (MQ=255)                   aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAaaaaaaaaaaa                                                                                                                                       >  2:93372/1‑149 (MQ=255)                   aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACt                                                                                                                                      <  1:452027/151‑1 (MQ=255)                     tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCACACAaaaaaaaaaaa                                                                                                                                       >  1:1170714/1‑147 (MQ=255)                          ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCTCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCATACCAAAAAAAAAAATTGAggt                                                                                                                                >  1:1135274/1‑148 (MQ=255)                           tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTg                                                                                                                               <  1:244448/150‑1 (MQ=255)                                gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCat                                                                                                                           <  1:798618/149‑1 (MQ=255)                                    tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAGTTTTTCCGACAAATAAAAAAAATCGAgggttcatg                                                                                                                          >  2:70471/1‑138 (MQ=255)                                        gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGaa                                                                                                                <  1:236296/151‑1 (MQ=255)                                         cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGaaa                                                                                                               <  1:537179/151‑1 (MQ=255)                                         cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGaaa                                                                                                               <  1:604232/151‑1 (MQ=255)                                          aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGaaa                                                                                                               <  1:698215/150‑1 (MQ=255)                                          aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAAAGGAGTTGCCAT‑GATGAGCGgaa                                                                                                               >  2:786241/1‑150 (MQ=255)                                             attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGta                                                                                                            <  1:694988/150‑1 (MQ=255)                                              ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGAGTTGCCAT‑GATGAGCGGAAGtaa                                                                                                           >  2:651770/1‑150 (MQ=255)                                               tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGtaat                                                                                                          <  2:1006914/150‑1 (MQ=255)                                                     ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCAT‑GATTGGCGGAAGtaataaaaaa                                                                                                    >  2:679639/1‑146 (MQ=255)                                                       aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAg                                                                                                 <  1:1128955/151‑1 (MQ=255)                                                         tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAgg                                                                                                <  1:831066/150‑1 (MQ=255)                                                            cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGt                                                                                            <  2:687327/151‑1 (MQ=255)                                                            cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGt                                                                                            <  2:1167911/151‑1 (MQ=255)                                                             gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                                           <  2:233093/151‑1 (MQ=255)                                                              cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGGGTTGCCAT‑GATGAGCGAAAGtaataaaaaa                                                                                                    >  1:519948/1‑137 (MQ=255)                                                              cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGa                                                                                          <  1:151464/151‑1 (MQ=255)                                                                  gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAAAAAAAAAAATGGAGGTTGCAT‑GATGAGCGAAAATAATAAAAAACAAGCAGAGaaaa                                                                                       >  1:858225/1‑148 (MQ=255)                                                                   aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGGCAT‑GATGTGGGGAAGTAATAAACAAAAAGGAGTGAAT‑aa                                                                                     >  2:789693/1‑150 (MQ=255)                                                                      cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATTGAGGTTGCAT‑GATGAGCGAAAAGAATAAACAAAAAGCAGTGGAT‑aa                                                                                     >  1:1104727/1‑147 (MQ=255)                                                                         gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAACAGGAGGTGCCAT‑TATGAGCGAAAAGAAAAAGCAACAAGCAGGGAAA‑AAgat                                                                                  >  2:135991/1‑145 (MQ=255)                                                                         gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGGGGTGCCAT‑GATGAGGGGAAGGAATAAACAACAGGCAGGGAAT‑aa                                                                                     >  1:679656/1‑144 (MQ=255)                                                                             tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGAGGTGGCAT‑GATGAGCGAAAATAATAAGCAAAAAGCAGTGAAT‑AAATTGaaa                                                                              >  1:1018136/1‑145 (MQ=255)                                                                              gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAACAAAAAAAAAAAATGGGGTTTGCAT‑TATGAGCGGAAGGAATAAACAAAAAGGAGGGAAT‑AAGTTGACa                                                                              >  1:307210/1‑146 (MQ=255)                                                                                tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGAtt                                                                        <  1:656162/150‑1 (MQ=255)                                                                                 aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGt                                                                      <  2:384535/151‑1 (MQ=255)                                                                                  aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTc                                                                     <  2:548632/151‑1 (MQ=255)                                                                                  aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTc                                                                     <  2:149339/151‑1 (MQ=255)                                                                                       atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCAT‑GATGAGCGAAAAGAATAAACAAAAGGCAGGGAAT‑AAATTTACAGAGATTGTCGCTa                                                                 >  2:58358/1‑150 (MQ=255)                                                                                       atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCAAACAAATAAAAAAAAAGGAGGTGCCCT‑TATGAGGGAAAGGAATAAGCAACAAGCAGGGAAT‑AAGTTGACAGAGAATGTTGCt                                                                  >  2:1143922/1‑149 (MQ=255)                                                                                        tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGGATTTTTTTTAACAAAAACAAAAAAAGGAGGTTGCTC‑GGTGAGGGACAGTAATAAACAACAGGCAACGAAT‑AAGGTGCCAGAGGTTGTCGGTaa                                                                >  1:280693/1‑150 (MQ=255)                                                                                         atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAAc                                                               <  2:877701/150‑1 (MQ=255)                                                                                         atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACt                                                              <  1:134809/151‑1 (MQ=255)                                                                                           tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCAGACAAAAAAAAAAAATGGAGTTGGCAT‑GGTTAGCGGAAGTAATAAACAACAAGCAGGGAAA‑AAATTGACAGAGAATGTCGCTAACttt                                                            >  1:1058695/1‑151 (MQ=255)                                                                                               ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTAcct                                                        <  1:251087/151‑2 (MQ=255)                                                                                               ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCg                                                        <  1:1159465/151‑1 (MQ=255)                                                                                                    cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGGGGTGCCAT‑GATGAGCGAAAGAAAAAAACAACAAGGAGGGAAT‑AAATTGACAGAGAAAGTCGCTAAATTTACCGcc                                                      >  2:272743/1‑148 (MQ=255)                                                                                                      aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACAAAAAATGGAGGTGCCCT‑GATGAGCGGAAGAAATAAACAAAAGGCAGGGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTATCGCCATGa                                                  >  1:650185/1‑150 (MQ=255)                                                                                                         aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACAAAAAATGGAGGTGCCAT‑GATGAGCGGAAGTAAAAAAAAACAAGCAGGGAAA‑AAATTGACAGAGATTTTCGCTAACTTTACCGCCCTGAttt                                               >  2:664097/1‑150 (MQ=255)                                                                                                          atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                                             <  2:1114000/151‑1 (MQ=255)                                                                                                             taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCAACAAAAACACAAACTGGAGGTGCCATGGTTGAG‑GGAAGTAAAAAACAAAAGGAGGCTAATAAAGTTG‑GAGAGATTGTGGCGAACTTTTCCGCCAttaatttt                                             >  1:364985/1‑141 (MQ=255)                                                                                                                 aCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCAT‑GATGAGCGAAAGTAATAAGCAACAAGGAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTAACGCCATGATTTTTAccc                                         >  2:913228/1‑148 (MQ=255)                                                                                                                    acaTTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCAACAAAAATAAAAAATGGAGGTGTCAT‑GATGGGGGAAAATAATAAGAAACACGCAGTGAAA‑AAATTGACAGAGATTGTCGCTCACTTTTCCGCCACGATTTTTTCCCCaa                                      >  1:691962/1‑148 (MQ=255)                                                                                                                    acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGGGTTGCCAT‑GATGAGCGAAAGTAAAAAACAAAAAGCAGGGGAT‑AAAATGACAGAGATTGTTGCTAACTTTTCCGCCCTGATTTTTTCCCCAATGc                                   >  2:916146/1‑151 (MQ=255)                                                                                                                    acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAATGGAGTTGCCAT‑GATGAGCGAAAGTAATAAACAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTCTAcccca                                       >  2:902810/1‑145 (MQ=255)                                                                                                                        ttttGGGGGTAGTTTGGGGGGGGAAATTTTTCCAGACAAAAACAAAAACGGGAGTTCCCAT‑GATGAGAGAAAAAAATAAGCAACGGTCAGTAAAT‑AAGTTGATAGAGTTTGTCGCAACCTTTACCGCCATGATTTCTACCCGAATGCCtg                                <  2:1244093/150‑1 (MQ=255)                                                                                                                                gTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAACTGGAGGTGGCCT‑GATGAGCGAAAAGAAAAAACAAAAAGCAGGGAAA‑AAATTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCCAATGCCTGATGACgtgg                       >  1:1122144/1‑151 (MQ=255)                                                                                                                                    ttgggggCGGTAATTTTTTCCAGACAAATAAAAAAAGTGGAGTTGCAAG‑GATGAGCAAAAGAAATAAGCAACAGGCAGTGAAT‑AAGTGGAGAGAGATTGTCGCTATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                    <  2:852757/145‑1 (MQ=255)                                                                                                                                     ttGTGGCGGTGATTTTTCCCAGAAAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                   <  1:1071477/150‑1 (MQ=255)                                                                                                                                        tgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                >  1:557536/1‑150 (MQ=255)                                                                                                                                        tgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                >  1:363893/1‑150 (MQ=255)                                                                                                                                             gTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa           >  1:1218296/1‑150 (MQ=255)                                                                                                                                                  tttttCCAGAAAAATACAAAAACGGGAGTGGCCAT‑GATGAGCAAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg      <  2:576012/150‑1 (MQ=255)                                                                                                                                                      tCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  1:753772/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                       CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206285‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑