Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8800TC65.2% 2.4 / 43.9 46K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (11/5);  new base C (0/30);  total (11/35)
Fisher's exact test for biased strand distribution p-value = 3.27e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.15e-07

ACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAG  >  NC_000913/357730‑358025
                                                                                                                                                           |                                                                                                                                                  
aCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAttttt                                                                                                                                                    >  2:1218880/1‑151 (MQ=255)
   cGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccg                                                                                                                                                 <  1:180967/151‑1 (MQ=255)
    gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgc                                                                                                                                                >  1:1254192/1‑151 (MQ=255)
      aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgccg                                                                                                                                              >  2:604139/1‑151 (MQ=255)
      aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgccg                                                                                                                                              >  2:145744/1‑151 (MQ=255)
        gCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGca                                                                                                                                            <  1:302631/151‑1 (MQ=255)
               aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc                                                                                                                                    <  1:527466/151‑1 (MQ=255)
                           tCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctctc                                                                                                                        >  2:1002227/1‑151 (MQ=255)
                              gcaaaTCCCAGCCGAGGCCTTGTGTCAGCGGCTCCCGTGATTCATTCCCGCAAACACCCGCGCTTTTATCCAACGCCATGTGCAAAAATGCCTCCGCGGCGGCTGTCTGCAAGTTATTTTTCCGCCGCAAAAAAAccgccg                                                                                                                               <  1:1185898/138‑1 (MQ=17)
                                   tcccACCCGCAGCCTGGTGCCGGGGGCTACGGTGTTTATTTCCCGCAAAAACCCGCGCTTTTACCCAACCCCTTGTAAAAAAATGCTTCCGGGGGGGCTGTCGGCCAGTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCagtagt                                                                                                                 <  2:1211666/148‑1 (MQ=255)
                                    tCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTgg                                                                                                               >  1:851415/1‑151 (MQ=255)
                                     ccggCCGGAGCCTTGTGCCGGCGGCTACCTTGATTTTTTCCCCCAAACACCCGCACATTTACCCACCCCCTTTGGAAAAATTGCCTCCGCGGCGGCTGTCTGCAAGTTTTTTTCCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTg                                                                                                                <  2:1015942/146‑1 (MQ=255)
                                      cAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCg                                                                                                             >  2:998059/1‑151 (MQ=255)
                                       aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                            >  2:97630/1‑151 (MQ=255)
                                         ccGCAGCCCTGGGCCAGCGCCTACCGTATTTCTTTCCCCCCAACACCCGCGCTTTTACCCACCGCCATGTGAAAAAATCCCTCCGGGGCGGTTGTCTCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                           <  2:642170/150‑1 (MQ=255)
                                         ccGCAGACCTGTTCAGCCGGAACCCTTATTTCATTCCCGCAAACACCCGCGATTTACCCCAACGCAAGGTACAAAATGCCTTGCGCGGGGGCTGTCTGCAACCTATTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                          <  2:1198551/151‑1 (MQ=255)
                                          cgcaacctggtgcCAGCGGCTCCCGTTTTTCATTCCCCCCAACACCCGCGCTTTAACCCAACGCCTTGTAAAAAAATCCCTCCGCGGCGGCTGTCGGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                          <  1:1209561/141‑1 (MQ=255)
                                          cgaagcccTGTGTCAGGGGCTCCCGTGTTTCTTTCCCGCCACCACCCGCGCTTTTACCAACCCCAATGTGAAAAATTGCCTCCGGGGCGGCTTTCTGCTAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc                                                                                                         <  2:1139967/145‑1 (MQ=255)
                                            cggccgtttgccggCGTCTACCGTAATTCTTTCCCCCCACCACCCGCGTTTTTACCCACCCCCATGAGAAAAAAGGCCTCCGGGGGGGTTTTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc                                                                                                         <  1:922202/136‑1 (MQ=255)
                                              gACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                            >  2:187327/1‑144 (MQ=255)
                                                       cggcgcCTACCGTAATTCTTTCCCCCCAACACCCCCCCTTTTATCCAACCCAATGTGCAAAAATGCCTTCGGGGCGGCTGTCGGCCCGTTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAg                                                                                              <  2:515583/147‑1 (MQ=255)
                                                           gcctccgttGATTCTTTCCCCCCAACACCCCCGCATTTACCCACCGCCATGTGAAAAAATCCCTCCGCGGCGGTTTCCGGCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATa                                                                                               <  1:115389/136‑1 (MQ=255)
                                                           gcctcccGTGATTCTTTCCCCCCAACACCCGCGCTTTTACCCACCGCCATGTGAAAAAATGCCTCCGGGGGGGCTGTCTGCCAGCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCt                                                                                            <  2:825477/142‑1 (MQ=255)
                                                              tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAGAttt                                                                                          >  1:584735/1‑146 (MQ=255)
                                                               cccGTGTTTCTTTCCCCCCAACACCCGCCCTTTTACCAACCCCAAGGTGAAAAAATCCCTCCGGGGGGGCTGTCGGCAAGTTATTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGccc                                                                                    <  1:139969/150‑1 (MQ=255)
                                                               cccGTGTTTCTTTCCCCCAACCACCCGCGCTTTTACCCACCGCCTGGTAAAAAAATGCCTCCGGGGCGGTTGTCGGCAAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGc                                                                                      <  1:1061724/148‑1 (MQ=255)
                                                               aCCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAAATTTaaaacc                                                                                    >  2:556077/1‑147 (MQ=25)
                                                                ccGTGTTTTTTTCCCCCCAACACCCGCGCATTTACCCAACGCCATGTGCAAAAATGCCTCCGGGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGccc                                                                                    <  2:159503/150‑1 (MQ=25)
                                                                ccGTGATTCTTTCCCCCCAACACCCGCGCATTTACCCACCGCCTTGTGAAAAAATGCCTTCGCGGCGGCTGTCGGCCAGTTTTTTTTCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGc                                                                                      <  2:788332/148‑1 (MQ=255)
                                                                   tGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGgaaaaagttt                                                                                          >  2:592861/1‑134 (MQ=25)
                                                                    gATTCTTTCCCCCCAACACCCGCCTATTTACCCACCCCCAGGTCCAAAAAGCCCTCCGGGGGGCTTGTCCGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGt                                                                                  <  1:171552/148‑1 (MQ=255)
                                                                       ttCTTTCCCGCAAACACCCGCGATTTTACCAAACGCCATGTGAAAAATTGCCTCCGGGGGGGTTGTCGGCCACTTATTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtg                                                                              <  2:452240/150‑1 (MQ=255)
                                                                             ccccccAACACCCCCCCTTTTACCCACCGCCATGTGAAAAAATCCCTCCGCGGCGGCTGTCTGCAAGCTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTtgtgtg                                                                       <  1:556026/147‑1 (MQ=255)
                                                                                       acccGCGCATTTACCCACCGCAATGTGAAAAAATCCCTTCGGGGCGGTTGTCTCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATc                                                                 <  2:845587/146‑1 (MQ=255)
                                                                                                tttATCCACCGCCATGAAAAAAAGTCCCTGCGCGGGGGTTTCCGCCCAGTTTTTTTCCCCCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGt                                                        <  1:786247/148‑1 (MQ=255)
                                                                                                      ccACCGCCATGTGAAAAAATGCCTCCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAt                                                  <  1:370226/149‑1 (MQ=255)
                                                                                                      ccAACGCCATGTGAAAAAATGCCTCCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACaa                                                   <  2:954857/148‑1 (MQ=255)
                                                                                                          cGCCATGTAAAAAAATGCCTTCGGGGCGGCTTTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGACAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtgt                                                <  2:14748/147‑1 (MQ=255)
                                                                                                            ccATGTGAAAAAAGCCCTCCGCGGCGGCTGTCGGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaaa                                           <  2:449158/150‑1 (MQ=255)
                                                                                                                     aaaaaTGCTTGCGTGGCGGATGACTGCAACAAATTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAg                                     <  2:462029/148‑1 (MQ=255)
                                                                                                                     aaaaaGGCCTGCGCGGCGGCTTTCTCCCATCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGt                                    <  1:568480/149‑1 (MQ=255)
                                                                                                                        aaaccctcccgcgCCGGTTGTCAGCCGTTTTTTTTCCCCCCCCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcgg                                <  1:278901/142‑1 (MQ=255)
                                                                                                                                  gggggggcTGTCGGCCGGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                          <  2:1066980/142‑1 (MQ=255)
                                                                                                                                      cggcTTTCCGCCAGCTATTTCCCCGCCACAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCa                   <  1:812295/150‑1 (MQ=255)
                                                                                                                                             cTGCCAGTTTTTTTCCCCCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAg                  <  1:1143432/144‑1 (MQ=255)
                                                                                                                                              ggccggttATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc               <  1:1218756/139‑1 (MQ=255)
                                                                                                                                                ccAGTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAcc              <  1:145731/145‑1 (MQ=255)
                                                                                                                                                 ccactttttttCCCACCCAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTa        <  1:1002127/145‑1 (MQ=255)
                                                                                                                                                    tatttttcCCGCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCa                         <  1:1230355/127‑1 (MQ=255)
                                                                                                                                                     ttttttcCCGCCAAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTaa       <  1:1064137/145‑1 (MQ=255)
                                                                                                                                                     ttttttcCCCCCGAAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAt      <  1:899604/146‑1 (MQ=255)
                                                                                                                                                      tttttcCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg    <  2:551856/148‑1 (MQ=255)
                                                                                                                                                       ttttcCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGa   <  2:25172/149‑1 (MQ=255)
                                                                                                                                                       ttttcCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAg  <  2:694655/150‑1 (MQ=255)
                                                                                                                                                           |                                                                                                                                                  
ACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAG  >  NC_000913/357730‑358025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: