Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8870CA44.0% 15.1 / 22.9 50L268F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (8/20);  new base A (0/22);  total (8/42)
Fisher's exact test for biased strand distribution p-value = 6.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATG  >  NC_000913/357738‑358037
                                                                                                                                                          |                                                                                                                                                        
gCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGca                                                                                                                                                         <  1:302631/151‑1 (MQ=255)
       aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc                                                                                                                                                 <  1:527466/151‑1 (MQ=255)
                   tCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctctc                                                                                                                                     >  2:1002227/1‑151 (MQ=255)
                      gcaaaTCCCAGCCGAGGCCTTGTGTCAGCGGCTCCCGTGATTCATTCCCGCAAACACCCGCGCTTTTATCCAACGCCATGTGCAAAAATGCCTCCGCGGCGGCTGTCTGCAAGTTATTTTTCCGCCGCAAAAAAAccgccg                                                                                                                                            <  1:1185898/138‑1 (MQ=17)
                           tcccACCCGCAGCCTGGTGCCGGGGGCTACGGTGTTTATTTCCCGCAAAAACCCGCGCTTTTACCCAACCCCTTGTAAAAAAATGCTTCCGGGGGGGCTGTCGGCCAGTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCagtagt                                                                                                                              <  2:1211666/148‑1 (MQ=255)
                            tCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTgg                                                                                                                            >  1:851415/1‑151 (MQ=255)
                             ccggCCGGAGCCTTGTGCCGGCGGCTACCTTGATTTTTTCCCCCAAACACCCGCACATTTACCCACCCCCTTTGGAAAAATTGCCTCCGCGGCGGCTGTCTGCAAGTTTTTTTCCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTg                                                                                                                             <  2:1015942/146‑1 (MQ=255)
                              cAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCg                                                                                                                         >  2:998059/1‑151 (MQ=255)
                               aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                                        >  2:97630/1‑151 (MQ=255)
                                 ccGCAGCCCTGGGCCAGCGCCTACCGTATTTCTTTCCCCCCAACACCCGCGCTTTTACCCACCGCCATGTGAAAAAATCCCTCCGGGGCGGTTGTCTCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                                       <  2:642170/150‑1 (MQ=255)
                                 ccGCAGACCTGTTCAGCCGGAACCCTTATTTCATTCCCGCAAACACCCGCGATTTACCCCAACGCAAGGTACAAAATGCCTTGCGCGGGGGCTGTCTGCAACCTATTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                      <  2:1198551/151‑1 (MQ=255)
                                  cgcaacctggtgcCAGCGGCTCCCGTTTTTCATTCCCCCCAACACCCGCGCTTTAACCCAACGCCTTGTAAAAAAATCCCTCCGCGGCGGCTGTCGGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                      <  1:1209561/141‑1 (MQ=255)
                                  cgaagcccTGTGTCAGGGGCTCCCGTGTTTCTTTCCCGCCACCACCCGCGCTTTTACCAACCCCAATGTGAAAAATTGCCTCCGGGGCGGCTTTCTGCTAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc                                                                                                                     <  2:1139967/145‑1 (MQ=255)
                                    cggccgtttgccggCGTCTACCGTAATTCTTTCCCCCCACCACCCGCGTTTTTACCCACCCCCATGAGAAAAAAGGCCTCCGGGGGGGTTTTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc                                                                                                                     <  1:922202/136‑1 (MQ=255)
                                      gACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                                        >  2:187327/1‑144 (MQ=255)
                                               cggcgcCTACCGTAATTCTTTCCCCCCAACACCCCCCCTTTTATCCAACCCAATGTGCAAAAATGCCTTCGGGGCGGCTGTCGGCCCGTTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAg                                                                                                          <  2:515583/147‑1 (MQ=255)
                                                   gcctccgttGATTCTTTCCCCCCAACACCCCCGCATTTACCCACCGCCATGTGAAAAAATCCCTCCGCGGCGGTTTCCGGCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATa                                                                                                           <  1:115389/136‑1 (MQ=255)
                                                   gcctcccGTGATTCTTTCCCCCCAACACCCGCGCTTTTACCCACCGCCATGTGAAAAAATGCCTCCGGGGGGGCTGTCTGCCAGCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCt                                                                                                        <  2:825477/142‑1 (MQ=255)
                                                      tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAGAttt                                                                                                      >  1:584735/1‑146 (MQ=255)
                                                       cccGTGTTTCTTTCCCCCCAACACCCGCCCTTTTACCAACCCCAAGGTGAAAAAATCCCTCCGGGGGGGCTGTCGGCAAGTTATTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGccc                                                                                                <  1:139969/150‑1 (MQ=255)
                                                       cccGTGTTTCTTTCCCCCAACCACCCGCGCTTTTACCCACCGCCTGGTAAAAAAATGCCTCCGGGGCGGTTGTCGGCAAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGc                                                                                                  <  1:1061724/148‑1 (MQ=255)
                                                       aCCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAAATTTaaaacc                                                                                                >  2:556077/1‑147 (MQ=25)
                                                        ccGTGTTTTTTTCCCCCCAACACCCGCGCATTTACCCAACGCCATGTGCAAAAATGCCTCCGGGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGccc                                                                                                <  2:159503/150‑1 (MQ=25)
                                                        ccGTGATTCTTTCCCCCCAACACCCGCGCATTTACCCACCGCCTTGTGAAAAAATGCCTTCGCGGCGGCTGTCGGCCAGTTTTTTTTCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGc                                                                                                  <  2:788332/148‑1 (MQ=255)
                                                           tGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGgaaaaagttt                                                                                                      >  2:592861/1‑134 (MQ=25)
                                                            gATTCTTTCCCCCCAACACCCGCCTATTTACCCACCCCCAGGTCCAAAAAGCCCTCCGGGGGGCTTGTCCGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGt                                                                                              <  1:171552/148‑1 (MQ=255)
                                                               ttCTTTCCCGCAAACACCCGCGATTTTACCAAACGCCATGTGAAAAATTGCCTCCGGGGGGGTTGTCGGCCACTTATTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtg                                                                                          <  2:452240/150‑1 (MQ=255)
                                                                     ccccccAACACCCCCCCTTTTACCCACCGCCATGTGAAAAAATCCCTCCGCGGCGGCTGTCTGCAAGCTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTtgtgtg                                                                                   <  1:556026/147‑1 (MQ=255)
                                                                               acccGCGCATTTACCCACCGCAATGTGAAAAAATCCCTTCGGGGCGGTTGTCTCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATc                                                                             <  2:845587/146‑1 (MQ=255)
                                                                                        tttATCCACCGCCATGAAAAAAAGTCCCTGCGCGGGGGTTTCCGCCCAGTTTTTTTCCCCCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGt                                                                    <  1:786247/148‑1 (MQ=255)
                                                                                              ccACCGCCATGTGAAAAAATGCCTCCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAt                                                              <  1:370226/149‑1 (MQ=255)
                                                                                              ccAACGCCATGTGAAAAAATGCCTCCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACaa                                                               <  2:954857/148‑1 (MQ=255)
                                                                                                  cGCCATGTAAAAAAATGCCTTCGGGGCGGCTTTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGACAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtgt                                                            <  2:14748/147‑1 (MQ=255)
                                                                                                    ccATGTGAAAAAAGCCCTCCGCGGCGGCTGTCGGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaaa                                                       <  2:449158/150‑1 (MQ=255)
                                                                                                             aaaaaTGCTTGCGTGGCGGATGACTGCAACAAATTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAg                                                 <  2:462029/148‑1 (MQ=255)
                                                                                                             aaaaaGGCCTGCGCGGCGGCTTTCTCCCATCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGt                                                <  1:568480/149‑1 (MQ=255)
                                                                                                                aaaccctcccgcgCCGGTTGTCAGCCGTTTTTTTTCCCCCCCCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcgg                                            <  1:278901/142‑1 (MQ=255)
                                                                                                                          gggggggcTGTCGGCCGGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                                      <  2:1066980/142‑1 (MQ=255)
                                                                                                                              cggcTTTCCGCCAGCTATTTCCCCGCCACAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCa                               <  1:812295/150‑1 (MQ=255)
                                                                                                                                     cTGCCAGTTTTTTTCCCCCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAg                              <  1:1143432/144‑1 (MQ=255)
                                                                                                                                      ggccggttATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc                           <  1:1218756/139‑1 (MQ=255)
                                                                                                                                        ccAGTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAcc                          <  1:145731/145‑1 (MQ=255)
                                                                                                                                         ccactttttttCCCACCCAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTa                    <  1:1002127/145‑1 (MQ=255)
                                                                                                                                            tatttttcCCGCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCa                                     <  1:1230355/127‑1 (MQ=255)
                                                                                                                                             ttttttcCCGCCAAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTaa                   <  1:1064137/145‑1 (MQ=255)
                                                                                                                                             ttttttcCCCCCGAAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAt                  <  1:899604/146‑1 (MQ=255)
                                                                                                                                             tttttccccgccgCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg                <  1:604086/143‑1 (MQ=255)
                                                                                                                                              tttttcCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg                <  2:551856/148‑1 (MQ=255)
                                                                                                                                               ttttcCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGa               <  2:25172/149‑1 (MQ=255)
                                                                                                                                               ttttcCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAg              <  2:694655/150‑1 (MQ=255)
                                                                                                                                                 ttcccgccgCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGtt            <  2:124324/147‑1 (MQ=255)
                                                                                                                                                       ccgcAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAAAATTTAACCCGCCCTGTTTTGTGGGCATTGCTGCTGGTAACAATATGGGAAGGGGAGAGGGGGGGAACAGACCCAGGACCCCGCCCAATGAGttt           >  2:73755/1‑144 (MQ=255)
                                                                                                                                                          caacaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAAGGAAAAAAAATTTAAGCCGTCCTGTTGTGTGGGGAACGCTGCTGGGGACAAAATGGGAAAGGGAGAGGGGGGGATCAGCCCCCCAACCCCGCCAATTGAGTTTGCCCCCATg  >  2:984988/1‑151 (MQ=255)
                                                                                                                                                          |                                                                                                                                                        
GCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATG  >  NC_000913/357738‑358037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: