Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913307,8210GT46.2% 41.7 / 20.9 39Y504* (TAC→TAA) ecpCECP production outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (4/17);  new base T (18/0);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.81e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGATATATTTTCCTGTATTGGCGTTGCTGTCGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGT  >  NC_000913/307671‑307971
                                                                                                                                                      |                                                                                                                                                      
agCGATATATTTTCCTGTATTGGCGTTGCTGTCGCCGTTGTTATAGCGCTGAATACCGTCCCGCAGGCCAAGCGAACCTAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTg                                                                                                                                                        <  2:518577/151‑1 (MQ=255)
 gCGATATATTTTCCTGTATTGGCGTTGCTGTCGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGt                                                                                                                                                       <  1:390077/151‑1 (MQ=255)
     tatatTTTCCTGTATTGGCGTTGCTGTCGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCTGTCATCATTGTa                                                                                                                                                      <  1:150432/148‑1 (MQ=255)
             ccTGTATTGGCGTTGCTGTCGCCGTTGTTATAGCGCTGAACACCGGCCCGCAGTCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGACGGTCATCATTGTAGCt                                                                                                                                                   <  1:22510/143‑1 (MQ=255)
              cTGTATTGGCGTTGCTGTCGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCCTTTTAgacggtggtg                                                                                                                                            >  2:640699/1‑140 (MQ=255)
                   ttGGCGTTGCTGTCGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATACTTTTAGcgggtg                                                                                                                                               >  1:179264/1‑136 (MQ=255)
                           gCTGTCGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTc                                                                                                                             <  1:548166/151‑1 (MQ=255)
                                cGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGGCATCATTTTAGCTGATGCTGAATGTGCCCAGCtttcacc                                                                                                                         >  2:385285/1‑145 (MQ=255)
                                  ccGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGGTGGTGGTGGATGTGGCCAGCTTTGACcac                                                                                                                       >  2:140360/1‑150 (MQ=255)
                                   cGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCGTCATTTTAGCTGATGGTTAATGCGccccggcttggacac                                                                                                                       >  1:654204/1‑137 (MQ=255)
                                          ataGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCCTTTTAGCTGATTGTTAATTGGTCCAGCCTCGACcccaagggggtt                                                                                                              >  2:668224/1‑143 (MQ=255)
                                                   aaTACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGa                                                                                                       <  1:560040/149‑1 (MQ=255)
                                                   aaTACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCCTCGACCACAGTGAGTTCAGGGTGAg                                                                                                      >  2:57536/1‑150 (MQ=255)
                                                      aCCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGGGCTCATTTTTGCTGATTCTGAAAGTGGCCCCCCTCCACCCCACTGAggtttaggtgagt                                                                                                     >  1:250318/1‑136 (MQ=255)
                                                                       gCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGtt                                                                                 <  2:195697/151‑1 (MQ=255)
                                                                        cGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTATCCGATGCTGGATGGGCCCCGCCTTGACCCCAATGGGTTCAGGGTTGGGGTGGCGCCGGTTGCAccg                                                                                   >  2:620814/1‑146 (MQ=255)
                                                                          aaCCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCTGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGATTGt                                                                               <  1:718538/150‑1 (MQ=255)
                                                                                aGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATc                                                                        <  2:241735/151‑1 (MQ=255)
                                                                                   tACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGCTGATGCTGAATGTGCCCCGCTTCTACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGCTTTCACgggt                                                                                 >  2:562117/1‑137 (MQ=255)
                                                                                   tACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCCGCTTCGACCACAATGAGTTCAGGGTGAGTGTGCCGCCCGTTGCACGGGTGTCGGGATCg                                                                       >  2:463/1‑149 (MQ=255)
                                                                                      cGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTTGCTGATGCTGAATGTGCCCCGCTTCGACCACAATGAGTTCAGGTTTAGTGTGCCGCCGGTTGCACGGTTGTCGGCATCGc                                                                      >  2:9999/1‑147 (MQ=255)
                                                                                          gTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCg                                                               <  1:397101/150‑1 (MQ=255)
                                                                                            aGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTAATTCTTTTAGCCGGTTCCGAATGTGCCCAGCTTCCACCACAGAGAGTTTAGGGTGAGTGTGCCGCCCATTGCACCGGTGTCGGCATCGCCACGTCGc                                                              >  2:532247/1‑149 (MQ=255)
                                                                                             gACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGTTGATGCTGAATGTGCCCAGCTTCGACCCCACTGAGGTTAAGGTGAGTGTGGCGCCGCGTGCCCGGGTGTCGGCTTCCCTCCCTCGc                                                              >  1:13178/1‑148 (MQ=255)
                                                                                                aTTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTTATCATTTTAGCTGATGCTGAAAGTGCCCAGCTTCCACCACAGGGAGTTCAGGGTTGGGGTGCCGCCGGTTGCAAGGTTGTCGGCATCGCTACCTCGCaaat                                                          >  2:617012/1‑147 (MQ=255)
                                                                                                aTTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGCTGATGCTGAATGTGCCCCGCCTCGACCACAATGAGATTAGGGTGAGTGTGCCGCCGGTTGCACCGTTGTCGGCCTCGCTACGGCGCaaat                                                          >  2:340954/1‑147 (MQ=255)
                                                                                                  tttGATAGTATTCTGCCGTGGAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATTCTGAATGTGCCCAGCTTCTACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAattgat                                                       <  1:506124/150‑1 (MQ=255)
                                                                                                      aTAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCCTTGTTGCTGATGCTGAATGTGTCCAGCCTCGCCCACAATGAGGTTAGGGTGTGAGTGTCGCCGGTTGTACGGTTGTCGGCATCGCTACGTTGCCattgtttggc                                                   >  2:513169/1‑148 (MQ=255)
                                                                                                        aGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAAt                                                <  2:483941/151‑1 (MQ=255)
                                                                                                            aTCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCCTTTTAGCTGGAGCTTAATGTGCCCAGCCTCGACCACAATGAGGTCAGGGTGAGGGTGCCCCCCATTGCACGGGTGTCGGCATCCCTACGTCGCAATTTATTGCCAATGCgg                                            >  2:621035/1‑151 (MQ=255)
                                                                                                             tCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCGTCAATGTTGCTGAGGGGGAATGTGCCCCCCATCTACCACAGAGAGGTCAAGGTGAGGGGGGCGCCGGGTTGACGGTTGTTGGCCTTGCCACGACGCCAATGAATGTCAATACggg                                           >  2:336980/1‑151 (MQ=255)
                                                                                                                gCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCgg                                            <  2:661247/147‑1 (MQ=255)
                                                                                                                  cGTGTAATAATGGCTGTTGTAACGGCGGTCATCCTTTTAGCTGATGCTGAATGTGCCCCGCTTCGACCACAATGAGGTCAGGGTGAGGGTGCCGCCGGTTGCACGGGTGTTGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGgtttt                                      >  2:415152/1‑151 (MQ=255)
                                                                                                                  cGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGCTGATGCTGAAAGTGCCCCGCCTCGACCACAGTGAGGTCAGGGTGAGGGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCCATGCGGGgtttt                                      >  2:711234/1‑151 (MQ=255)
                                                                                                                  cGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGttttt                                      <  2:371421/151‑1 (MQ=255)
                                                                                                                  cGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGttttt                                      <  1:627187/151‑1 (MQ=255)
                                                                                                                        ataatGGCTGTTGTAACGGCGGTCAACCTTTTTGCTGGTGCTTAAAGTGCCCCGCCTTGACCACCGGGAGGTCAGGGTGAGTTGGCCGCCCATTGTACGGGTTGCGGGCTCCCTACCGCGCCAATGATTTGCACTGCGGGgttttt                                     >  1:513923/1‑146 (MQ=255)
                                                                                                                           atGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTa                             <  2:348073/151‑1 (MQ=255)
                                                                                                                                   tgtAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCcaca                      <  2:446811/150‑1 (MQ=255)
                                                                                                                                             gTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTaaa            <  1:513090/150‑1 (MQ=255)
                                                                                                                                                      gTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCgg   >  2:102776/1‑150 (MQ=255)
                                                                                                                                                      gTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCgg   <  2:40032/150‑1 (MQ=255)
                                                                                                                                                      gTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGt  <  1:140324/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AGCGATATATTTTCCTGTATTGGCGTTGCTGTCGCCGTTGTTATAGCGCTGAATACCGGCCCGCAGGCCAAGCGAACCAAAGGTACCGCTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGT  >  NC_000913/307671‑307971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑