Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913650,4640AT40.6% 45.4 / 11.4 32R9R (CGT→CGA) citEcitrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (1/18);  new base T (13/0);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 4.03e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGT  >  NC_000913/650316‑650614
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ccATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACg                                                                                                                                                      <  2:579811/151‑1 (MQ=255)
  aTGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACgtt                                                                                                                                                    <  1:215001/151‑1 (MQ=255)
  aTGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACgtt                                                                                                                                                    <  1:647586/151‑1 (MQ=255)
     cggcggGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACgttgtt                                                                                                                                                 <  2:569441/151‑1 (MQ=255)
                gtCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGc                                                                                                                                       <  1:234054/150‑1 (MQ=255)
                gtCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTGCAGCGaa                                                                                                                                         >  2:117722/1‑148 (MQ=255)
                 tCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTGCAGCGAAGCgg                                                                                                                                     >  2:128464/1‑151 (MQ=255)
                    ttttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCgg                                                                                                                                     <  2:523789/148‑1 (MQ=255)
                    ttttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTGTAACGAAACg                                                                                                                                      >  1:189199/1‑147 (MQ=255)
                        cACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTCGTTGTTGCAGCGGAGCGGaaaac                                                                                                                                >  2:2167/1‑147 (MQ=255)
                        cACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCa                                                                                                                               <  2:142074/150‑1 (MQ=255)
                                   aCGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAAtcc                                                                                                                     <  2:1115/149‑1 (MQ=255)
                                    cGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGCAGCGAAGCGGGAATCATTGGGAAacctcc                                                                                                                  >  2:36741/1‑151 (MQ=255)
                                             cGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGGCGCGGAGCGGGAAATATTTGCCAACCTCCCCTgg                                                                                                             >  2:87452/1‑147 (MQ=255)
                                                  tCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTTGTTGTTGCAGCGAAACGGGAAACATTTGCAATCCTCCCCTGGCAGAGCCgg                                                                                                    >  1:261376/1‑151 (MQ=255)
                                                       cATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGAGGTTTTTGATTTTGGAGCGAAGCGGGAATCATTGGCAATCCTCCCCCGGAAGGGCCCGGGTAc                                                                                               >  1:218108/1‑151 (MQ=255)
                                                         tCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTTCGTTGTTGCAGCGAAACGGAAATCATTGGCAATCCTCCCCTGGCAGAGCCGGGATaac                                                                                              >  1:353741/1‑148 (MQ=255)
                                                             ggCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTgg                                                                                         <  1:460414/151‑1 (MQ=255)
                                                               cATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTTGTTGTTGCAGCGAAGCGGGAAACATTGGCAATCCTCCCATGGCAGAGCCGGGATACCCCTgg                                                                                         >  2:276019/1‑149 (MQ=255)
                                                                     ccGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGCTTTCGTTGTTGCAGCCAAGACGGGAGCACTGGGCAACCTTCCATCGCTGAGCCGGGGTACCGCTGgcg                                                                                       >  1:13912/1‑145 (MQ=255)
                                                                      cGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCca                                                                                <  1:6139/151‑1 (MQ=255)
                                                                      cGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTTGTTGTTGCCGGGCAACGGGAAACATTGGGGAGTCTCCCCTCGGCGAGCCGGGGTAACCCTGgcgccggcc                                                                                 >  2:449057/1‑145 (MQ=255)
                                                                           aGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAgg                                                                           <  2:582088/151‑1 (MQ=255)
                                                                                   gAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAg                                                                   <  1:713090/151‑1 (MQ=255)
                                                                                              cATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGAGGTTTTCGTTGTTTCAGCGAAACGGAAAACATTGGCAATCCCCCCCCGGCAGAGCCGGGGGACCGCCGGCGCGTGCCAGCAAGGCTTCCCGTCGTGCAc                                                           >  2:474413/1‑148 (MQ=255)
                                                                                                             aCCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTGTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTg                                         <  1:419449/151‑1 (MQ=255)
                                                                                                               cAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTgcg                                       <  1:230880/151‑1 (MQ=255)
                                                                                                                        acaacaTGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCa                              <  2:232747/151‑1 (MQ=255)
                                                                                                                            caTGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGa                          <  1:587237/151‑1 (MQ=255)
                                                                                                                                       gggTGAGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATGCCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGGGCCTTTGTCATCGACATTCAGATGt               <  1:22272/151‑1 (MQ=255)
                                                                                                                                              aGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAcgccgc        <  1:36487/151‑1 (MQ=255)
                                                                                                                                                   tACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACg   >  2:617973/1‑151 (MQ=255)
                                                                                                                                                    aCGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGt  >  2:125702/1‑151 (MQ=255)
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CCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGT  >  NC_000913/650316‑650614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑