Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	718,690	0	T	G	40.5%	57.7 / 12.1	37	T591P (ACC→CCC)	speF	ornithine decarboxylase isozyme, inducible
Reads supporting (aligned to +/- strand):  ref base T (7/15);  new base G (15/0);  total (22/15)
Fisher's exact test for biased strand distribution p-value = 2.08e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTCAGCAG  >  NC_000913/718545‑718837                                                                                                                                                  |                                                                                                                                                    tCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATaa                                                                                                                                                 <  2:378921/150‑1 (MQ=255)  cACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAAcc                                                                                                                                               <  2:657880/151‑1 (MQ=255)   aCCGCGTAAATAGGCGTAGTTTGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGtata                                                                                                                                                  <  1:249125/147‑1 (MQ=255)        gTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGt                                                                                                                                          <  1:310149/150‑1 (MQ=255)        gTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTa                                                                                                                                         <  2:243802/151‑1 (MQ=255)             tAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCAGAACAATAATGTATTTTCTTTCAAAAATAACGCAGGGGATaaaccg                                                                                                                                             >  2:159289/1‑138 (MQ=255)                              ttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATAATGCATTTTCTTACAACAATCACGCAGGGGATAACCCCCGGGGGGCGCTTCATGTCGTTTGt                                                                                                                     >  1:97666/1‑149 (MQ=255)                                 cGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACAAGCATTTTCTTACCCAACTGACGCCGGGGATAACCGGCCGAGGGCTCTTCATTCTGTTTTTAgt                                                                                                                  >  2:640849/1‑148 (MQ=255)                                   gATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATGCATTTCCTTACACCACAGACGCAGGGGATAAAAGGCGGAGCGCCCTTCATGCTGTTTTTAGAt                                                                                                                 >  2:409055/1‑148 (MQ=255)                                    aTTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAATTCATTTCCTCACACAAACGACGCAGGGGATAAACGGCGGAGCGCTCCTTTTTCTGTTTTTAGGTgg                                                                                                               >  2:163468/1‑149 (MQ=255)                                      tCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAAAAATCATTTCCTTACCCAAACAACGCAGGGGATAAAACGCGGAGCGCTCTTTCTTCTGttttt                                                                                                                     >  1:210430/1‑139 (MQ=255)                                        aTGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTACACCACTGACGCAGGGGATAAACGGCGGAGCGCCCTTCCTGCTTTTTTTTGATTGAAg                                                                                                            >  2:117843/1‑148 (MQ=255)                                         tGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACAAGCATTTTCTTACCACACCGACGGCGGGGATAAACCGCGTGGGGCTCTTCATTCTGTTTGTTGAgggga                                                                                                             >  1:711197/1‑144 (MQ=255)                                               cgcgTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATTCAAAACATTCAATTCCTTACACAAATGACGCGGGGGATAACCGGCGTAGCGCTCTTCCTGCTGTTTGTAGATGGGAGGGAGGAcct                                                                                                   >  2:44441/1‑148 (MQ=255)                                               cgcgTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTc                                                                                                  <  1:656413/151‑1 (MQ=255)                                                  gTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGc                                                                                               <  2:579133/151‑1 (MQ=255)                                                        aGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATgg                                                                                         <  1:323811/151‑1 (MQ=255)                                                              ccTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTc                                                                                   <  1:9566/151‑1 (MQ=255)                                                               cTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTACAAAAAATCCATTTTCTGACCCAACTGAAGCCGGGGATAAACCGCGGAGCGCTCTTCCTGCTGTTTGTTGATGGAAGGGAGGACTTCTTCTaa                                                                                            >  1:519298/1‑141 (MQ=255)                                                               cTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAATACATTTCCTTACACAAATGACGCAGGGGATAAACCGCGTGGCGCCCCTCCTGCTGTTTTTAGATGGGAGGGAGCACATCTTCTAATGGGGGGTCg                                                                                  >  2:413068/1‑151 (MQ=255)                                                                      aaCATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCGTACAAAATATACATTTTCTTACCCAACTTATGCCGGGGGTAAACGGCGGAGGGCTCCTTTTTCTGTTTGTTGGTGGAAGGTGGGACTTTTGCTTAta                                                                                          >  1:424769/1‑135 (MQ=255)                                                                      aaCATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCaa                                                                           <  1:412979/151‑1 (MQ=255)                                                                       aCATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTACACAAATTACGCCGGGGATAAACCGCGGAGCGCCCTTCCTTCTGTTTTTTGATGGGAGGGAGGACTTCTTCTTATGGCGCGGCGGGCTTaa                                                                           >  2:94691/1‑150 (MQ=255)                                                                             ctTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAg                                                                       <  2:267747/148‑1 (MQ=255)                                                                               ttttGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATGCATTTTCTGACACAACTGACGCAGGGGATAAACGGCGGAGCGCTCTTTCTTCTGTTTTTTGATTGGAGGGAGGACTTTTGCTAATGGGGCGGCGGGCTCAAGCAGgtttt                                                                  >  2:551648/1‑151 (MQ=255)                                                                                    cAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTCCTGACACAACTGACGCAGGGGATAACCGGCGGAGCGCCCTTCATGCTGTTTGTAGATGGAAGGGAGCACTTCTTCTAATGGCGCGGCGGGCTCAAGCAGgtttt                                                                  >  2:200347/1‑146 (MQ=255)                                                                                              aCGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACcagca                                                   <  2:699912/151‑1 (MQ=255)                                                                                               cGTTGTGGCGGGCATTCAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACcagcag                                                  <  1:208704/151‑1 (MQ=255)                                                                                                    tgGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGca                                              >  2:331419/1‑150 (MQ=255)                                                                                                          gCATACAAATCATGCATTTCCTGAAACAACTGACGCAGGGTATAACCGACGTAGCGCTCTTCATGCTGTTTGAAGAAGGAAGGTAGAACTTATGCTAATGGCGCGGCGGACTCAAGCAGTTTTTCGAAGCGTACCCGCAGGGCGACAAGt                                        >  1:107717/1‑150 (MQ=255)                                                                                                                 aaTCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAg                                <  1:276612/151‑1 (MQ=255)                                                                                                                 aaTCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAg                                <  2:685456/151‑1 (MQ=255)                                                                                                                    cATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGttt                             >  2:309957/1‑151 (MQ=255)                                                                                                                       gCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGc                          >  1:61732/1‑151 (MQ=255)                                                                                                                         aTTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGcc                         >  2:5727/1‑150 (MQ=255)                                                                                                                                         taCGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGt        >  1:182354/2‑151 (MQ=255)                                                                                                                                          aCGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTc       <  1:408965/151‑1 (MQ=255)                                                                                                                                                 gTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTcagcag  >  1:371303/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTCAGCAG  >  NC_000913/718545‑718837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑