Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,224,025	0	C	A	39.0%	96.2 / 10.2	41	intergenic (+118/+254)	ycgI/minE	pseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base C (2/23);  new base A (16/0);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 1.48e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT  >  NC_000913/1223880‑1224175                                                                                                                                                  |                                                                                                                                                       ggTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGa                                                                                                                                                   <  1:283894/151‑1 (MQ=255)  gTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGaa                                                                                                                                                  <  2:645937/151‑1 (MQ=255)        ggTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAACTCACCCTATAGGGGAaaaaaaaaaaa                                                                                                                                               >  1:566351/1‑141 (MQ=255)           gtgAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAAt                                                                                                                                         <  1:244760/151‑1 (MQ=255)              aaCTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAACTCATCCCATGGGGGAAAAAATAaaaatgaaaatg                                                                                                                                       >  2:226204/1‑150 (MQ=255)                  ggTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCgg                                                                                                                                    <  2:627033/149‑1 (MQ=255)                     cGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGtt                                                                                                                                <  2:531709/150‑1 (MQ=255)                        tCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTatc                                                                                                                             <  1:134680/150‑1 (MQ=255)                           gACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTatca                                                                                                                            <  2:675290/148‑1 (MQ=255)                               aTCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAACCCGACCCATGGGGGAAAAAAGAAAAATAAAAATGCCGGGGTTTCATCcccc                                                                                                                      >  2:542376/1‑150 (MQ=255)                                  aaCGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAACTCAATCTATGGGGGAAAAAAAAATAATGAAAATTGGGGGGTATTATCTccc                                                                                                                      >  1:11787/1‑147 (MQ=255)                                      aTTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAACCCATACTCTTGGGGAACAATGACTAATGAAAATGAGGGGGTATCATCTCCCAGtat                                                                                                                 >  1:12244/1‑148 (MQ=255)                                          aaCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATa                                                                                                          <  2:384646/151‑1 (MQ=255)                                          aaCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATa                                                                                                          <  2:187386/151‑1 (MQ=255)                                             tGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGGAAACTCATCCTATGGGGGAAAAAAAAATAAAGAAATTGGGGGGGTGTTATCTCCCAATAAATACATa                                                                                                          >  1:255328/1‑148 (MQ=255)                                                   tCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAAt                                                                                                  <  1:672289/150‑1 (MQ=255)                                                    cGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGGAAACTCATTCCCTTGGGGAAAAAAAAATAAATAAAATGGGGGGGTATTATCTCCCCGGATAATCATACTAAAAATa                                                                                                 >  1:195020/1‑150 (MQ=255)                                                    cGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATaa                                                                                                <  2:85015/151‑1 (MQ=255)                                                          aGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGGAAACTCATTCTTTTGGAGGAAAAAGAAAAAATAAAATGGGGGGGTATTATCTCCCCGTATATTCAaaataaaaataa                                                                                                >  1:10579/1‑145 (MQ=255)                                                          aGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTa                                                                                           <  1:59888/150‑1 (MQ=255)                                                           gTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAACTCATTCTATGGGAGAAAAAAGAAAAAAGAAATTGCGGGGGTATCATCTCCCAGTATATCCATACTAACAATAAAGTTAtt                                                                                         >  1:192111/1‑151 (MQ=255)                                                             tttCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAACTCATCCTATGGGGGAAAAAAGAATAAAGAAATTTCGGGGGTATCATCTCCCCGTATATCCCTACTAAAAATAAGGTTAttt                                                                                        >  2:421168/1‑150 (MQ=255)                                                              ttCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTa                                                                                       <  2:205466/150‑1 (MQ=255)                                                               tCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAACACATCCTATTGGAGAAAAAAGAATAATGAAAATTCGGGGGTATCATTTCCCAGTATATCCATACTAACAAAAAAGGTAttt                                                                                        >  1:354052/1‑148 (MQ=255)                                                                cGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACt                                                                                     <  1:53724/150‑1 (MQ=255)                                                                cGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCCATGGGAGAAAAAAGAATAATGAAAATGCGGGGGTATCATCTCCCAGTATATCCATAATAACAATAAGGGTATTTaat                                                                                     >  2:415150/1‑148 (MQ=255)                                                                cGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAACTCATCCCATGGGGGAAAAATGAATAATGAAAATGCGGGGTTATCATTTCCCCGTATATTCATACTAACAAAAAAGGTAttt                                                                                        >  1:327350/1‑147 (MQ=255)                                                                 gTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTc                                                                                    <  2:204219/150‑1 (MQ=255)                                                                   ttACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAACTCATCCTTTGGGGGAAAAAAGAATAATGAAAATTCGGGGGTTTCATCTCCCCGTATATTCATACTAAAAATAAAGTTATTTACTcca                                                                                  >  2:85663/1‑148 (MQ=255)                                                                    tACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAAc                                                                                 <  2:652731/150‑1 (MQ=255)                                                                      cAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGGAAAACCATCCCATGGGGGAAAAAAGAATAAATAAAATGGGGGGTTATCATCTCCCAGAATATTACTACTAACACTAAAGGTAtttttt                                                                                     >  1:453998/1‑141 (MQ=255)                                                                               gAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATaa                                                                        <  1:241502/148‑1 (MQ=255)                                                                                   gggAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACAtttt                                                                 <  2:271656/151‑1 (MQ=255)                                                                                      aaaaTTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGGAACCTCATCCTATGGGAGAAAAAAGAAAAATGAAAATGCGGGGGTATCATTTCCCCGTATAACCACACTAACAAAAAGGGTATTTTCTCAACCACGCATAAACAttttgtt                                                              >  1:186789/1‑151 (MQ=255)                                                                                              ggggCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTtgtg                                                         <  1:495475/148‑1 (MQ=255)                                                                                               gggCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTg                                                     <  1:68558/151‑1 (MQ=255)                                                                                                  cAAAAAAAGCCCGCCAGTTACGGCGGGAAAACCCATCCTATGGGGGGAAAAAGAATAATTAAAATTCGGGGTTATCATTTCCCAATATATCCACACTTAAAATAAGGTTATTTTTTCAAACAGGCACTAACAATTTTTTTTTTGCGTggg                                                   >  2:209904/1‑150 (MQ=255)                                                                                                     aaaaaGCCCGCCAGTTACGGCGGGGAACCTCATACTATGGGGGAAAAAAGAAAAATTAAAATGCGGGGGTATTCTCTCCCCGGATAATCATAATAACAAAAAAGTTTTTTAATCCAACACGCATAAACAATTTTTTTTTTGGGTgg                                                    >  1:119553/1‑146 (MQ=255)                                                                                                                         ggggAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggggg                           >  2:44412/2‑151 (MQ=255)                                                                                                                                       cTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGc              <  2:374708/150‑1 (MQ=255)                                                                                                                                             gagaACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgagg       <  1:666640/151‑1 (MQ=255)                                                                                                                                               gaACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgaggag     <  2:192149/151‑1 (MQ=255)                                                                                                                                                  cAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAt  <  1:139526/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                       GGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT  >  NC_000913/1223880‑1224175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑