| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,933,409 | 0 | T | C | 52.4% | 15.3 / 24.6 | 21 | E399G (GAA→GGA) | edd | 6‑phosphogluconate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (6/4); new base C (0/11); total (6/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-03 | |||||||||||
CCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCT > NC_000913/1933278‑1933555 | ccTGGTTCTCAACCTGCACGTCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACCCTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTTTCGAGTGATTTTTCCGCCCCTTCCCGcca < 1:211799/148‑1 (MQ=255) cAACCGGCACGGCAGAGGTTTTCATACCCGCACGCCCCAGGTTCCCGCTTAACCTTTTTGTCCCACCATGATGAGAAAAAGGTTTTTCGAAGGAAGCGATCACATTGCTGTCGAGTGTTTTTTCCGCCCCTTCCCGCCAGTCCAGTTc < 2:674488/148‑1 (MQ=255) caGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGcc > 1:344028/1‑150 (MQ=255) gtttttAACAACCGCCCGGCCCAGTTTCCCGTTTACCTCTTTTGCCCCCCCATGATGAGAGAAAGGTTGTTCGAAGAAAGCGATCAATTTGCTGTCGAGTAATTTTCCCGCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCa < 1:81563/145‑1 (MQ=255) aaCCGCCCGCCCCAGGTTCCCGTTAAACCCTTTTGCCCCCCCAGGATAAGAAAAGGGTTTTCCGAGGGAAGGTATCACTTTGGTGTGGAGTAATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGt < 2:646577/150‑1 (MQ=255) aaCCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAAAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGTTTTTTCCGCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGt < 2:80174/150‑1 (MQ=255) cccggttccccGTTTAACCCTTTGGTCCCACCATGATGAGAGAAAGGTTGTTCGAGGGAAGCGATCACTTTGCTGTCGAGTAATTTTCCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagaca < 1:400211/142‑1 (MQ=255) ccGCTTACCACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCaaa < 2:535006/149‑1 (MQ=255) ccGCTAAACATTTTTGCCCCACCAGGATGAGAGAAAGGTTGTTCGAAGGAAGCGACCACATTGCTGTCGAGTGATTTTCCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAAc < 1:448876/151‑1 (MQ=255) cttttttccccccccATAATGAAAAAAGGTTTGTCCGAAGGAGGAGACAACTTTGCTGTGGATTATTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCTTGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCAc < 1:464954/141‑1 (MQ=255) tttGCCCCCCCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTGGGGTATTTTTCCCGCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGt < 2:35933/149‑1 (MQ=255) tGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTg > 2:343631/1‑151 (MQ=255) atgaGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATgc > 1:140556/1‑151 (MQ=255) agagaAGGGTTGTCCGAAGGAAGCGATCAATTTGCTGTCGGTTGTTTTTCCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATgcag < 1:423861/150‑1 (MQ=255) agaAAGGTTGTTCGAAGGAAGCGATCACATTCCTGTCGGGTTTTTTTCCCGCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATgcagc < 1:228416/149‑1 (MQ=255) aaggtttgttgGAAGAAAGCAATCAATTTTCTGTGGTGTGATTTTCCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATgc < 1:382541/139‑1 (MQ=255) aaaGGTTGTTCGAAGGAAGCGATCACATTGCTGTGGAGTGATTTTCCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAgg < 2:51675/150‑1 (MQ=255) aggaagCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGc > 1:665451/1‑149 (MQ=255) gCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCAc > 2:172080/1‑151 (MQ=255) ttGCTGTCGAGTGATTTTCCCGCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGa < 2:389954/150‑1 (MQ=255) tGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAAc > 1:217548/1‑151 (MQ=255) tttttCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCt > 1:198642/1‑151 (MQ=255) | CCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCT > NC_000913/1933278‑1933555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |