Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9110GA40.0% 46.1 / 19.1 41intergenic (‑122/+199)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base G (21/3);  new base A (0/16);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.54e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGC  >  NC_000913/2001763‑2002059
                                                                                                                                                       |                                                                                                                                                    
acCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGa                                                                                                                                                     >  2:658576/1‑150 (MQ=255)
 ccTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGaaa                                                                                                                                                   <  1:302448/151‑1 (MQ=255)
            ataGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccgcc                                                                                                                                        >  2:15791/1‑151 (MQ=255)
            ataGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccgcc                                                                                                                                        >  1:145093/1‑151 (MQ=255)
                agTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTg                                                                                                                                    >  2:346284/1‑151 (MQ=255)
                   gAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCg                                                                                                                                 >  2:401547/1‑151 (MQ=255)
                            aaaaaCGGCCCTGCTTTATATGAGTTAGCGGCATGTTTATCCGTTTCTGCGGGTTTTTTAAGCGGACGATTAGTGGGTAAAAGGAGGGTTTATTGGGGGGTTAAAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCa                                                                                                                         <  2:477292/148‑1 (MQ=255)
                              aaacgccctggctttATATGATTTATCGGGATGTTTATCCGTTTCTGCAGGTTTTTAAATCTGACGTTTAGTGGGTAAAAGGAGGGGTTATTGGGGGGTTAAAGGAAATTCCCGGACAAAAAAAACCCCCGCCGGTGGCGGGGAAGCAc                                                                                                                        <  1:516297/136‑1 (MQ=255)
                              aaaCAGCCCTGCGTTATATGAGTTACCGACATAATTATCCGTTTCTGGAGGTTTTTTAAGCGGACGATTGGTGGGTGAAAGGAGGGGTTTTTGGGGGGTTAAAGGTAAATCCCAGCCAGAAAAAAACCCCGCCGGTGGCGGGGAAGc                                                                                                                          <  1:166032/147‑1 (MQ=255)
                               aaCAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGtt                                                                                                                     >  2:188961/1‑151 (MQ=255)
                                         cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                           >  1:586158/1‑151 (MQ=255)
                                              tatGAGTTTCCGGATTGATTATCCGTTTCTGCGGGTTTTTAAATCGGACGATTAGTGGGTGAAAGGGGGGTTTATTGGGGGGTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgc                                                                                                        <  1:376965/149‑1 (MQ=255)
                                                         ggCATGTTTCTCCTTTTCCGGAGGTTTTTTAAGCGAACGATTAGTGGGTAAAAGGGGGGGTTTTTTGGGGGTTACGTTAAATTCCCAGTAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTg                                                                                             <  1:73961/149‑1 (MQ=255)
                                                                       tttCTGCGGGGTTTTTAATCGGACAATTGGTGGGTAAAATGGGGGGTTATTTGGGGGTTACAGGAAAATCCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggcg                                                                                 <  1:17880/147‑1 (MQ=255)
                                                                       tttCTGCAGGGTTTTTAATCGGACGATTGGGGGGTAAAATGGGGGTTTTTTTGGGGTTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTa                                                                               <  1:222736/149‑1 (MQ=255)
                                                                       tttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                              >  2:533730/1‑150 (MQ=255)
                                                                       tttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                              >  2:84982/1‑150 (MQ=255)
                                                                            gcggggTTTTTAAGCGGAGGATTAGTGGTTGAAATGGGGGTTTTTTGGGGGGTTAAAGGAAATTCCCAGAAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCt                                                                        <  1:29070/148‑1 (MQ=255)
                                                                              aGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                       >  1:206263/1‑150 (MQ=255)
                                                                               ggttttttAATCGGACGATTAGTGGGTAAAATGGGGGTTTATTGGGGGGTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                       <  2:262905/146‑1 (MQ=255)
                                                                                                 taagggggTGAAAGGAGGGGTTATTTGGGGGTAAAAGGAAAATTCCAGGCACAAAAACACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                           <  1:363295/138‑1 (MQ=38)
                                                                                                  tAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                                  >  1:460968/1‑151 (MQ=255)
                                                                                                   aGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                                  >  1:60281/1‑150 (MQ=255)
                                                                                                    gTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCa                                                 >  2:370589/1‑150 (MQ=255)
                                                                                                        gTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt                                             >  1:686972/1‑150 (MQ=255)
                                                                                                          gAAATGGGGTTTTTTTTGGGGTTTACAGTTAAATTCCAGACAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgtg                                                   <  1:710263/142‑1 (MQ=37)
                                                                                                             aTGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                        >  2:344171/1‑150 (MQ=255)
                                                                                                               gAGGGGTTTTTGGGGGGTTACAGTTAAATCCAAGACAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAt                                     <  1:401455/151‑1 (MQ=37)
                                                                                                                            ggggtttaaagaaaaTTCCCGGGCAAAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAt                               <  1:360697/133‑1 (MQ=25)
                                                                                                                            ggggTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACtt                        <  2:481829/151‑1 (MQ=255)
                                                                                                                             ggttaaaaGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                       <  1:553801/144‑1 (MQ=25)
                                                                                                                              gtttACAGGAAAATTCCGGGCAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                      <  2:320318/149‑1 (MQ=255)
                                                                                                                                ttACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGAGGCGGGGAAGCACGTTGCTGACAAATTGAGCTTTATGTTGCCGGAAGCGGCGCAAACGCCTTATCCGGCCTGCAAAAATGTGCAAATTGAATAAATTGCAATTCAAc                          >  1:520384/1‑145 (MQ=255)
                                                                                                                                   cAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGcc                 >  2:129710/1‑151 (MQ=255)
                                                                                                                                    aGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt                >  2:599846/1‑151 (MQ=255)
                                                                                                                                     ggaaaaTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg               <  2:111897/148‑1 (MQ=255)
                                                                                                                                     ggTAAATTCAAGCAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt                <  1:658489/150‑1 (MQ=255)
                                                                                                                                      gTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg               >  1:580641/1‑150 (MQ=255)
                                                                                                                                        aaaTCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa            <  2:180189/151‑1 (MQ=255)
                                                                                                                                         aaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa            >  2:259527/1‑150 (MQ=255)
                                                                                                                                                      aGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcgc  >  2:594506/1‑150 (MQ=255)
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ACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGC  >  NC_000913/2001763‑2002059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: