Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,252,6570CT42.9% 40.5 / 20.1 35L320F (CTT→TTT) yeiIputative kinase
Reads supporting (aligned to +/- strand):  ref base C (5/15);  new base T (15/0);  total (20/15)
Fisher's exact test for biased strand distribution p-value = 4.87e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGC  >  NC_000913/2252512‑2252804
                                                                                                                                                   |                                                                                                                                                     
gCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGaa                                                                                                                                                  <  1:443100/151‑1 (MQ=255)
  aaCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAgg                                                                                                                                                <  1:264843/151‑1 (MQ=255)
      gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAGCTTTTTTTAAgg                                                                                                                                                >  2:173037/1‑147 (MQ=255)
      gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATa                                                                                                                                             <  1:708245/150‑1 (MQ=255)
             tATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAGATTTTTTTTAGGAAACAGttt                                                                                                                                      >  2:384814/1‑149 (MQ=255)
                     cGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAgc                                                                                                                            <  2:456912/151‑1 (MQ=255)
                                  tATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGTGATAGCTTTTTTTTAGGAGACAGTTTTCgcgcccgcgccccggtttcgg                                                                                                               >  1:393267/1‑142 (MQ=255)
                                       cAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGTTTTTTTTTAGGATAAAGATTTCGCGACAACGCCCGCTTTGTgggtgcc                                                                                                          >  2:221930/1‑146 (MQ=255)
                                        aGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCt                                                                                                         <  2:673496/151‑1 (MQ=255)
                                          cGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATATCTTTTTTTTAGGGTAAAGATTTCGCGCCAGCGCCCCTTTTTCGGttggc                                                                                                          >  1:7622/1‑144 (MQ=255)
                                           gAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGCTTTCTTTTAGGGTAAAGTTTCCGCGCCAGCGCCCGCTTTTCgggtgcc                                                                                                          >  2:394583/1‑142 (MQ=255)
                                           gAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTgc                                                                                                       <  1:496578/150‑1 (MQ=255)
                                              aaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAACTTTCTTTTAGGATAAAGTTTTCGCGACAGCGCCCCCTTTTTGGGAGCCcgcgcg                                                                                                   >  2:488715/1‑150 (MQ=255)
                                                 gATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCaa                                                                                               <  2:77499/151‑1 (MQ=255)
                                                  aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAAt                                                                                              <  2:289279/151‑1 (MQ=255)
                                                         aCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTcgcgc                                                                                       <  1:642664/151‑1 (MQ=255)
                                                                ttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAACTTTTTTTAAGGGTAAAGTTTTCCCGCCAGCGCCCGCTTTGCGGTAGGCTGCGCGGGAAATTcgcgc                                                                                       >  2:513475/1‑144 (MQ=255)
                                                                        cgcgCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGAGATAGCTTTTTTTAAGGGTAAAAGTTTTGCGACCGCGCCCGTTTTTTGGTGGCCTGCGCGGCAATTTTTCGCGCCCGCGGCAggct                                                                         >  2:550080/1‑147 (MQ=255)
                                                                          cgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAAATTTTTTTTAGGATAAAATTTTCCCGACAGCGCCCGGTTTTCGGGAGGCCGCGCGGGCAATTTTCGCGCCCGCGGCCGcctta                                                                       >  2:695538/1‑146 (MQ=255)
                                                                            ccAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGTATATAACTTTTTTTTAGGGTACAAGTTTCCCGGCAGCCCCCGTTTTTTGGGAGCCTGCGCGGCCAATTTGCGCGCCCGCGGCCGCTTtaa                                                                      >  2:597510/1‑149 (MQ=255)
                                                                                 gcATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAaccct                                                               <  1:706213/151‑1 (MQ=255)
                                                                                  cATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAAATTTTTTTTAGGATACAGTTTTCCCGACAACGCCCCGTTTTCGGTAGCCTCCGCGGCAAATTTTCGCGCCCGCGGCAGCCTAAACAaccc                                                                >  2:22743/1‑149 (MQ=255)
                                                                                             aGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGATTTTTTTGAGGGTAAAGTTTTCGCGCCAGCGCCCGGTTTTGGGGAGCCCGCGCGGCCAATTTGCGCGCCCGCGGCCGCCTTAACAacaccaccccctcttcc                                                   >  1:329926/1‑143 (MQ=255)
                                                                                             aGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGACCGGGATATAGCTTTTTTTCAGGGAAAAAGTTTCCCCACAGCGCCCCTTTTTCGGGAGCCCGCGCGGCTACTTTTCGCCGCCCCCGCAGCCTAAAAAAACCCACCCTGTc                                                       >  2:348450/1‑147 (MQ=255)
                                                                                              gACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTCTATATTCTTTTTTTATGGTACAGGTTTTCCGCCAGGGCCCGGTTTTCGGGAGCCTGGGCGGCAAATTTTTGCGCCCGCCGCCGCCTAaacaaaaccaccc                                                           >  1:321612/1‑134 (MQ=255)
                                                                                              gACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGcct                                                  <  2:694516/151‑2 (MQ=255)
                                                                                                   ttttGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTTTAACTTTTTTTTAGGATACAGTTTTCGCGACAGCGCCCGCTTTTTGGTCGCCTCCGCGGCAATTTTTCTCGCCCGCCGCGGCCTCAACAaacctacccctgcttccgcaa                                               >  1:370764/1‑137 (MQ=255)
                                                                                                    tttGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTTTTTTAGGAAACAGTTTTCCCGGCAACGCCCGCTTTTTGGGAGGCTGCGCGGCCATTTTCCGCGCCCGCCGCAGCTTTAAAAaacccaccccctcctccgctaa                                              >  2:246236/1‑136 (MQ=255)
                                                                                                      tGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAAcgc                                           <  2:555056/150‑1 (MQ=255)
                                                                                                       ggTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACgcgc                                         <  1:384724/151‑1 (MQ=255)
                                                                                                                       ttATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCaa                          <  1:125493/150‑1 (MQ=255)
                                                                                                                           ggCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgtg                     >  2:363712/1‑151 (MQ=255)
                                                                                                                                  cTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAAt              >  1:596290/1‑151 (MQ=255)
                                                                                                                                  cTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAAt              >  1:23374/1‑151 (MQ=255)
                                                                                                                                                 ttCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGc  <  2:343239/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGC  >  NC_000913/2252512‑2252804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: