Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,678,0430CT42.9% 20.3 / 14.4 28E107K (GAA→AAA) yphEputative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (2/14);  new base T (12/0);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 5.98e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGT  >  NC_000913/2677894‑2678193
                                                                                                                                                      |                                                                                                                                                      
ctgctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTc                                                                                                                                                        <  1:493005/150‑1 (MQ=255)
            cGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGc                                                                                                                                           <  2:402984/151‑1 (MQ=255)
             gggCGTAGCGTTGAAACAAGTTGTTTAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAAACAATCATGCCCTTGCGGCGGAGCCACTCCCCGAGGCCCGAGGGTTTccccgctagaag                                                                                                                                             >  1:676747/1‑139 (MQ=255)
                   aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGGTTTTCCCCCCTTTCAGCCCCTccc                                                                                                                                    >  2:487334/1‑150 (MQ=255)
                             cAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGAGG‑CAGAGGGTTTCCGCCCCTGTCAGCCCTTCCACCAGACtt                                                                                                                            >  2:273681/1‑149 (MQ=255)
                                 ttgttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGAGG‑CAGAGGGTTTTCCCCCCTGTCCCCCCCTCCCCCCCACTTAAATc                                                                                                                       >  1:694167/1‑150 (MQ=255)
                                       aGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATaa                                                                                                                <  1:566179/151‑1 (MQ=255)
                                          ctgagcatCAACGCCCACAGCCGGGAACCATCGTCGGCCATCCTGCGCCGTTTCGCGGTGATCAAGCCGTCCGTTGGGGATGGCCCACTGCCCGATG‑CGGAGGTTTTCCGCCACTGTCAGGCCTTCCACCAGTCTTACCGCCTGATa                                                                                                                 <  1:298143/140‑1 (MQ=255)
                                             aaCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCGACCGGGG‑CAGCGGGTTTCCGCCACCGCCCGGCCCTCCCCCACACCTAAATCCTTGTaaa                                                                                                               >  1:79222/1‑146 (MQ=255)
                                                  cAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCa                                                                                                      <  2:529663/150‑1 (MQ=255)
                                                  cAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCCAGG‑GAGAGGGTTTTCGCCACCGTCAGCCCCTCCACCACACTTTAATCCTCATAAACCCCGCGCAc                                                                                                     >  2:248550/1‑151 (MQ=255)
                                                   aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCCAGG‑CAGAGGGTTTTCGCCCCCTCCAGCCCTTCCCCCCCCACTAACTCCCGCTAAAACGCGCGcccc                                                                                                    >  1:158490/1‑151 (MQ=255)
                                                     cGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCCAGG‑CAGAGGGTTTTCGCCCCTTGTAGCCCTTCCCCCCAACTTAAACCCTGATAAACcgcgccagcccc                                                                                                  >  1:407530/1‑144 (MQ=255)
                                                             gcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAg                                                                                          <  1:184785/151‑1 (MQ=255)
                                                                   aaGCACCGTTGGGCTTCATGCGCCATTGGCAGTTAATCAATCAGACCATGCCTGCGGGCCCACTGACCGAGG‑CAGAGTTTTTCCGCCACTGTCAGCCCTTCCCCCAGGCGTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCa                                                                                     <  1:670944/150‑1 (MQ=255)
                                                                     gCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACgg                                                                                  <  2:239596/151‑1 (MQ=255)
                                                                      cAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACg                                                                                   <  1:253559/149‑1 (MQ=255)
                                                                        aCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGAGG‑CAGAGGGTTTTCGCCCCCGTCAGCCCCTCCACCAGACTTAAATCCTGATAAAACGCGCGCACCCCCAGTTCAGCGGCACgg                                                                                  >  2:231682/1‑148 (MQ=255)
                                                                             gggCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCa                                                                           <  1:60456/150‑1 (MQ=255)
                                                                                 aTCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGGTTTTCCCCCCCGTCAGCCCTTCCACCCGACTTTACTCCTGATAAAACCCGCGCACCCCCCGTTCAGCGGCACGGCGAGGCa                                                                           >  2:510305/1‑146 (MQ=255)
                                                                                 aTCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCCAGG‑CAGAGGGTTTTCGCCCTTGTCATCCCTTCCACCCGAATTAACTCCTTATAAACCGCGCGCACCCCCAGTTCTGCGGCCCGGCGAgggcgcgt                                                                       >  1:611451/1‑143 (MQ=255)
                                                                                  tCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGAGG‑CAGAGGGTTTTCGCCCCTGTCAACCCTTCCACCAGACTTAACTCCTGATAAAACCCGCGCCCCCCCCGTTCACCGGCACGGCGAGt                                                                             >  2:365036/1‑143 (MQ=255)
                                                                                      gcgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTc                                                                 <  1:28396/151‑1 (MQ=255)
                                                                                                  ggTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCa                                                      <  2:115924/150‑1 (MQ=255)
                                                                                                    tAATCAATCATGCCGTTGCGGCGGGGCCACTGACCCAGG‑CAGAGGGTTTTCGCCCCTGTCAGCCCTTCCCCCAGACTTAACTCCTGATAAAACCCGCGCACCCCCCGTTCAGCGGCACGGCGAGGCAGCGTAGCTTCGTCACCtt                                                         >  2:673200/1‑145 (MQ=255)
                                                                                                         aatcaTGCCGTTGCGGCGGGGCCACTGACCCAGG‑GAGAGGGTTTTCCCCCCTGTCAACCCCTCCACCCGACTTTAATCCTGATAAACCACGCGCCCCCCCAGCTCAGCGGGCCGGCGAGGGAGCGCAGGTTCTTCACCTTcc                                                       >  1:605449/1‑142 (MQ=255)
                                                                                                                 cGTTGCGGCGGGGCCACTGACCGCGG‑CAGAGGGTTTTCCCCACCGTCCGCCCCTCCCCCAGACTTAACTACTGATAAAACCCGCGCACCCCCCGTTCAGCGGCCCGGCGGGGCAGCGGAGCTTCGTCACCTTCCCCTCGCGTCTCGcc                                         >  1:448350/1‑148 (MQ=255)
                                                                                                                       ggcggGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGat                                <  2:602179/151‑1 (MQ=255)
                                                                                                                             gCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCAcc                          <  2:661392/151‑1 (MQ=255)
                                                                                                                                   gACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTAt                     <  1:377180/150‑1 (MQ=255)
                                                                                                                                                      ccGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGt  >  2:99784/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGG‑CAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGT  >  NC_000913/2677894‑2678193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑