Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	668,691	C→A	G9* (GGA→TGA)	rlmH ←	23S rRNA m(3)Psi1915 pseudouridine methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	668,691	0	C	A	100.0%	126.9 / NA	33	G9* (GGA→TGA)	rlmH	23S rRNA m(3)Psi1915 pseudouridine methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/17);  total (16/17)

ACCCTCTTTGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCC  >  NC_000913/668545‑668835                                                                                                                                                   |                                                                                                                                                 aCCCTCTTTGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACg                                                                                                                                              >  1:26827/1‑151 (MQ=255)                 aTGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCt                                                                                                                              <  1:251230/151‑1 (MQ=255)                     gcTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCAc                                                                                                                          <  2:57022/151‑1 (MQ=255)                        tGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGc                                                                                                                        <  1:171752/150‑1 (MQ=255)                         gATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCAt                                                                                                                      <  2:364347/151‑1 (MQ=255)                          aTGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCAtt                                                                                                                     >  2:457765/1‑151 (MQ=255)                                         ccGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGttttt                                                                                                      <  1:175633/151‑1 (MQ=255)                                          cgcgTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTc                                                                                                     >  1:63326/1‑151 (MQ=255)                                            cgTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCa                                                                                                   <  1:128194/151‑1 (MQ=255)                                                      ccggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATAc                                                                                           >  1:622261/1‑149 (MQ=255)                                                        ggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGc                                                                                       <  2:365604/151‑1 (MQ=255)                                                         gAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCGGAGTTTTTCCAGTTCATACAGGCg                                                                                      >  2:301541/1‑151 (MQ=255)                                                                    aGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCtt                                                                           <  1:137171/151‑1 (MQ=255)                                                                    aGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCtt                                                                           >  1:19786/1‑151 (MQ=255)                                                                       tCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCt                                                                        >  2:559110/1‑151 (MQ=255)                                                                        cGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCt                                                                        <  2:175456/150‑1 (MQ=255)                                                                                   tCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATgg                                                             >  2:115295/1‑150 (MQ=255)                                                                                        cGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAAt                                                        <  2:549193/150‑1 (MQ=255)                                                                                             aaCGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATc                                                  >  2:212610/1‑151 (MQ=255)                                                                                               cgacgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGc                                                <  1:656004/151‑1 (MQ=255)                                                                                                 acgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGccc                                              >  2:37626/1‑151 (MQ=255)                                                                                                   gCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaa                                            <  1:559997/151‑1 (MQ=255)                                                                                                                aaaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATc                               >  1:660853/1‑151 (MQ=255)                                                                                                                  aaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCa                             <  1:644195/151‑1 (MQ=255)                                                                                                                     cGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTc                          >  2:461311/1‑151 (MQ=255)                                                                                                                     cGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTc                          >  2:599033/1‑151 (MQ=255)                                                                                                                       gTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCgg                        <  1:689398/151‑1 (MQ=255)                                                                                                                                 aGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTc              <  1:337596/151‑1 (MQ=255)                                                                                                                                 aGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTc              <  2:63330/151‑1 (MQ=255)                                                                                                                                     cGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCt          >  2:154540/1‑151 (MQ=255)                                                                                                                                     cGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCt          <  2:431251/151‑1 (MQ=255)                                                                                                                                     cGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCt          >  1:350624/1‑151 (MQ=255)                                                                                                                                                ttCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGcc  >  2:416262/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                 ACCCTCTTTGTCGAGTATGCGCTTGATGTCCGCATTCTTGCCGCGTTTTCCGGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCC  >  NC_000913/668545‑668835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑