Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,496	0	T	A	43.0%	114.0 / 38.6	79	intergenic (+301/‑155)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (36/9);  new base A (0/34);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 2.44e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTG  >  NC_000913/29346‑29641                                                                                                                                                          |                                                                                                                                                  tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                   >  1:289354/1‑151 (MQ=255)    aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATaaa                                                                                                                                                <  2:870371/151‑1 (MQ=255)       gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATaaaaaa                                                                                                                                             <  1:996644/151‑1 (MQ=255)       gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                              >  1:904105/1‑150 (MQ=255)        tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                            >  2:324191/1‑151 (MQ=255)          aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATcc                                                                                                                                          <  1:1009544/151‑1 (MQ=255)             tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCg                                                                                                                                        <  1:690839/150‑1 (MQ=255)                 aCCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAtt                                                                                                                                   >  2:893390/1‑151 (MQ=255)                 aCCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                                    >  1:600858/1‑150 (MQ=255)                    aTTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                  >  1:412154/1‑149 (MQ=255)                     tttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGt                                                                                                                               >  1:926950/1‑151 (MQ=255)                      ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                              >  1:854308/1‑151 (MQ=255)                      ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                              >  1:853599/1‑151 (MQ=255)                          tCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACt                                                                                                                          >  2:1099431/1‑151 (MQ=255)                                 ttttCTGCTCTTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCTTTTTCA‑AGAA‑CAGGTTAGATGATTTTTTTGTCGCTTAATGCC‑TGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAgc                                                                                                                    <  1:1016904/150‑1 (MQ=255)                                 ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                                   >  2:98345/1‑151 (MQ=255)                                  tttCTGCTTTTTTTTTTTTCATCAATTTTTCTTGCTGCGAAGGCTTTTCCA‑AAAA‑CAGGTTAGATTTTTTTTTTGTCG‑TTAAAGCCTTGAAAAACTTGAAGGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAgcg                                                                                                                   <  2:364643/150‑1 (MQ=255)                                   ttCTGCTTTTTTTTTTTTCATCAATTTTTCTTGCTGCAAAAGCTTTTCCA‑AAAA‑AAGGTAAAATAATTTTTTTGTCGTTTAATGCC‑TAAAAAACATGAATGAGCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAgcgc                                                                                                                  <  2:734619/150‑1 (MQ=255)                                   ttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGcc                                                                                                                 >  2:604100/1‑151 (MQ=255)                                    tCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                >  1:443625/1‑151 (MQ=255)                                     cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                >  2:950047/1‑150 (MQ=255)                                     cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                >  2:493966/1‑150 (MQ=255)                                       gCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                              >  2:48953/1‑150 (MQ=255)                                       gCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                              >  1:287066/1‑150 (MQ=255)                                           tttttttttTCATGAAATCTTTTTGCTGCGAAAGCTTTTCCA‑AAAA‑CAGGTTAGATATTTTTTTTGTCGTTTAATGCC‑TGTAAAACTTGCATGACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCata                                                                                                             <  2:770974/146‑1 (MQ=255)                                           gTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                         >  2:205589/1‑151 (MQ=255)                                              tttATTTCATGAATTCTTCTTGCTGCGCAAGCTTTTTCA‑AGAA‑CAGGTTAGATGATTTTTTTGTCGTTTAATGCC‑TGAAAAACATGCAGGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCa                                                                                                       <  1:804821/150‑1 (MQ=255)                                                tATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                     >  1:320363/1‑150 (MQ=255)                                                  tttcatcaatttttcttGCTGCCAAAGCTTTTCCA‑AAAA‑AATGTTAGATAATTTTTTTGTCGTTTATTGCT‑TAAAAAACATGCTGGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                   <  2:46171/138‑1 (MQ=255)                                                            tcttctTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTg                                                                                        >  2:803476/1‑151 (MQ=255)                                                                cttCCTGAGCAAGCTTTTGCA‑AAAG‑CGGGTAAGATGTTTTTTTTGCCGTTTAGTCCT‑TAAAAAACTTACAGGCCCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGc                                                                                       <  1:899272/148‑1 (MQ=255)                                                                   gctgcGCAAGCGTTTCCA‑AGAA‑CAGTTAAGATGATTTTTTTGTCGCTTAATGCC‑TGAAAAACTTGCATGACCAACAAAAAAAAATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                         <  1:925661/143‑1 (MQ=255)                                                                     tgcGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                               >  1:768304/1‑151 (MQ=255)                                                                     tgcGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                               >  2:334896/1‑151 (MQ=255)                                                                       cgcAAGCTTTTTAA‑AAAA‑CGGTTTAAATTATTTTTTTGTCTTTTAAGCCT‑TAAAAAACATGCAGGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaaa                                                                                 <  2:168960/147‑1 (MQ=255)                                                                       cgcAAGCTTTTCCC‑AAAA‑CAGTTTAGATGATTTTTTTGCCGTTTAATGCC‑TTAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                  <  1:538561/146‑1 (MQ=255)                                                                        gaaaGCTTTTCCA‑AAAA‑CAGTTAAGATGATTTTTTTGTCTCTTATTGCT‑TTAAAAAAATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaaa                                                                                 <  1:517551/144‑1 (MQ=255)                                                                        cgcaattgtttgaaaGAA‑CAGTTTAAATAATTTTTTTGTCGTTTAATGCT‑TAAAAAACAGGCATTACCCACAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcg                                                                             <  2:562912/137‑1 (MQ=255)                                                                          aaGCGTTTTCC‑AGAA‑CAGGTTAGATGATTTTTTTGTCGCTTAATGCC‑TAAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                              <  1:556690/147‑1 (MQ=255)                                                                              gTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                       >  2:1061191/1‑150 (MQ=255)                                                                                tttCCAAGAA‑CAGGTTAGATGATTTTTTTGTCGTTTAATGCG‑TGAAAAACATGAATAACCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                                      <  1:290629/150‑1 (MQ=255)                                                                                tttCC‑AAAACCAGGTAATTTGATTTTTTTTTCGCTAAGTGCC‑TTAAAAACTGGCCCGACCAAAAAAAAAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                        <  1:454582/148‑1 (MQ=255)                                                                                ttccc‑AGAA‑CGGGTTAGATTATTTTTTTGTCTTTTAATGCT‑TGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAg                                                                     <  1:171096/147‑1 (MQ=255)                                                                                  tCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGCAAGc                                                                    >  1:1060895/1‑149 (MQ=255)                                                                                       aaaacggtttAGATGATTTTTTTGCCGCTAATGGCG‑TGTAAAACAGGCATGGCCAAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                                 <  1:48964/141‑1 (MQ=255)                                                                                         a‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                            >  1:913297/1‑151 (MQ=255)                                                                                            aGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                          >  1:55075/1‑151 (MQ=255)                                                                                             ggTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                         >  2:1057951/1‑151 (MQ=255)                                                                                               ttAGATGATTTTTTTGTCGTTTAATCCC‑TAAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                        <  1:37765/150‑1 (MQ=255)                                                                                               ttAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                        >  2:865255/1‑150 (MQ=255)                                                                                                    tatttttttttTCGTTTATTGCC‑TGTAAAACATGCAGGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                         <  2:195666/139‑1 (MQ=255)                                                                                                       tttttttGTCGTTAAATCCT‑TGAAAAAAATGCATGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                                   <  2:213149/145‑1 (MQ=255)                                                                                                        cTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                               >  2:555772/1‑150 (MQ=255)                                                                                                        cTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                               >  1:1105828/1‑150 (MQ=255)                                                                                                         ttttttTCGTTTAATGCT‑TTAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                               <  1:379649/149‑1 (MQ=255)                                                                                                                ctcTTAATGCC‑TAAAAAACATGCATGACCAAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                         <  2:306619/146‑1 (MQ=255)                                                                                                                cGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                      >  1:869868/1‑151 (MQ=255)                                                                                                                     aaTGCC‑TTTAAAAAATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                     <  1:1132408/147‑1 (MQ=255)                                                                                                                      aatccctaaaaaaCATGCAGGACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                 <  2:754965/142‑1 (MQ=255)                                                                                                                         gcttaaaaaaCATGCATACCCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                                <  2:412049/143‑1 (MQ=255)                                                                                                                         cc‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGTCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                             >  2:265895/1‑151 (MQ=255)                                                                                                                          cctaaaaaaCATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                               <  1:1020372/144‑1 (MQ=255)                                                                                                                          c‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                            >  2:218365/1‑151 (MQ=255)                                                                                                                               aaaaCTTGCATGAACCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                            <  1:950362/147‑1 (MQ=255)                                                                                                                               aaaaCATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                            <  1:1158032/147‑1 (MQ=255)                                                                                                                               aaaaCATGCATGACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                            <  1:1099769/147‑1 (MQ=255)                                                                                                                                aaaCTTGCATGACCAAAAAAATAAAAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                       <  2:727134/151‑1 (MQ=255)                                                                                                                                 accatgcatgACCAACAAAAAAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                      <  2:244329/149‑1 (MQ=255)                                                                                                                                  aCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                      >  1:291389/1‑150 (MQ=255)                                                                                                                                      gcatgAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                 <  1:324269/151‑1 (MQ=255)                                                                                                                                        atgACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                  <  1:34896/148‑1 (MQ=255)                                                                                                                                          gggcaaaaaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga                <  2:289286/141‑1 (MQ=255)                                                                                                                                          gaccaaaaaaaTAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga                <  2:703310/141‑1 (MQ=255)                                                                                                                                          gAGCAACAAAAAAAAATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt              <  1:494110/150‑1 (MQ=255)                                                                                                                                           acccAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag                   <  1:202744/142‑1 (MQ=255)                                                                                                                                           aGCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag               <  2:790113/148‑1 (MQ=255)                                                                                                                                                caAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt       >  2:945691/1‑151 (MQ=255)                                                                                                                                                  aaaTAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct     >  1:769303/1‑151 (MQ=255)                                                                                                                                                    aaaataAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct   <  2:853328/149‑1 (MQ=255)                                                                                                                                                     taataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg  >  1:349237/1‑151 (MQ=255)                                                                                                                                                          |                                                                                                                                                  TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTG  >  NC_000913/29346‑29641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑