Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,846,674	0	T	G	42.9%	90.5 / 14.3	65	T247P (ACC→CCC)	hycD	hydrogenase 3, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (12/24);  new base G (27/0);  total (41/24)
Fisher's exact test for biased strand distribution p-value = 8.32e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCC  >  NC_000913/2846526‑2846822                                                                                                                                                      |                                                                                                                                                       aaGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGttt                                                                                                                                                      <  1:73555/151‑1 (MQ=255)   gCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTcc                                                                                                                                                    <  2:533555/151‑1 (MQ=255)     cAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGTAAGGTTTc                                                                                                                                                     >  1:996654/1‑149 (MQ=255)       aaCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCAttt                                                                                                                                                <  2:633242/151‑1 (MQ=255)       aaCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCAttt                                                                                                                                                <  2:20070/151‑1 (MQ=255)        aCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCAttt                                                                                                                                                >  1:678850/1‑150 (MQ=255)          ccagcccagGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTTCAttttt                                                                                                                                              >  2:318483/1‑148 (MQ=255)            agcccagGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGCG‑AAGGGTTCCAttttttcc                                                                                                                                           >  2:806919/1‑146 (MQ=255)                     aaTGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATa                                                                                                                                 <  1:857623/151‑1 (MQ=255)                      aTGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCCTTTTTCCCCCCGG‑AATa                                                                                                                                 >  2:945651/1‑150 (MQ=255)                        gcgcGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTTTTCCCCCGG‑AATaaa                                                                                                                               >  2:324334/1‑150 (MQ=255)                           cgGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTTTTCCCCCGG‑AAAAAAAAcc                                                                                                                           >  2:613438/1‑151 (MQ=255)                           cgGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTTTCCCCACGG‑AATAAAAAcc                                                                                                                           >  2:574330/1‑151 (MQ=255)                            ggAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACAccc                                                                                                                          <  1:723826/151‑1 (MQ=255)                             gAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGCG‑GAAGTATCCCTTTCGCCCCACGC‑AATaaaacccac                                                                                                                         >  2:597280/1‑145 (MQ=255)                               gTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCcga                                                                                                                       <  1:174175/151‑1 (MQ=255)                                 aaTATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAAGTTTCCATTTTTCCCCCCGGCAAAAAAccccccac                                                                                                                       >  1:744312/1‑148 (MQ=255)                                  atatCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACAcccccccg                                                                                                                     >  2:579675/1‑146 (MQ=255)                                  atatCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTTTTCCCACGG‑GATAAAAAcccc                                                                                                                         >  1:176855/1‑146 (MQ=255)                                  atatCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTGTCCCCACGG‑AATAAACACCCccac                                                                                                                      >  1:191125/1‑149 (MQ=255)                                   tatCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCCTTTGTCCCCCCCG‑AATAAAAAcccc                                                                                                                         >  2:523514/1‑145 (MQ=255)                                   tatCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTGTCCCCCCGG‑GATAAACAcccc                                                                                                                         >  1:474917/1‑145 (MQ=255)                                    atCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGAG‑AAGGATTTCCTTTGTTCCCCCGG‑AAAAAAAACCCccacaca                                                                                                                   >  2:437356/1‑147 (MQ=255)                                       aaGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTTTTCCCCCCG‑AATAAAAAcccccccga                                                                                                                    >  2:83335/1‑141 (MQ=255)                                             cAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCCGCaa                                                                                                          >  2:373101/1‑150 (MQ=255)                                                 cGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑GAGGGTTTCATTTTTCCCCCCGG‑AAAAAAAAACCCCCCCCACATCTGCAAaacc                                                                                                      >  2:321864/1‑150 (MQ=255)                                                  gggCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAAcaccac                                                                                                    <  2:491151/151‑1 (MQ=255)                                                   ggCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACaccacc                                                                                                   <  1:558187/151‑1 (MQ=255)                                                    gCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACAccacca                                                                                                  <  1:479487/151‑1 (MQ=255)                                                          cTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTTTCCCCCCCG‑GAAAAACACCCCCACGAACATCTGCCACACCACCAGCTgct                                                                                            >  2:1000828/1‑149 (MQ=255)                                                            gTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGCG‑AAGGGTTCCATTTGTCCCCCCCG‑GATAAACACCCCCCCCAACATCTGCCACACCCCCACCTGtt                                                                                            >  2:636808/1‑149 (MQ=255)                                                                   aaCAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGa                                                                                   <  1:893787/151‑1 (MQ=255)                                                                   aaCAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTTTCCCCACGG‑AATAAAAACCCCCACGAACAACTGCAACACCACCAGCTGttt                                                                                           >  2:348843/1‑143 (MQ=255)                                                                       gcgcGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑cc                                                                              <  1:523668/151‑1 (MQ=255)                                                                           gATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTA‑AAAGGTTTCATTTGTACCCCCGG‑AATAAAAACACCCACTAACACCTGCTAAAACACCAGCGGGTTTAGGTCGATA‑cccc                                                                            >  1:746347/1‑149 (MQ=255)                                                                                ccAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCAt                                                                     <  1:695120/151‑1 (MQ=255)                                                                                ccAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTGTCCCCCCGG‑AATAAACACCCCCACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCCttt                                                                        >  1:105336/1‑148 (MQ=255)                                                                                 cAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTTTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCCGCCGTTTCAGGCTGATA‑CCCCCTTTCAt                                                                     >  1:250016/1‑150 (MQ=255)                                                                                    gACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCCTTTTTCCCCCCGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCCGTTTCAGGCTGATA‑CCCCCTTTCATGAc                                                                  >  1:405940/1‑150 (MQ=255)                                                                                          gACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTTCCTTTTTCCCCCCGG‑GATAAACACCCCGCCGAACAACTGCAACACCACCAGCTGTTTTAGGCTGGTA‑CCCCATTTCATGACGccca                                                             >  1:1042281/1‑147 (MQ=255)                                                                                          gACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAg                                                           <  2:697159/151‑1 (MQ=255)                                                                                            ccaccaGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAgcc                                                         <  2:474780/151‑1 (MQ=255)                                                                                             caccaGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCCTTTTTCCCCCCGG‑AAAAAAAACCCCCACGAACATCTGCCAAACCCCCACCCGTTTCAGGCCGGTA‑CCCCATTTTCTGACGCCCAAgcc                                                         >  2:139440/1‑150 (MQ=255)                                                                                                caGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACAACCCGACGAACATCTGCAACACCACCAACTGTTTCAGGCTGATA‑ACCCATTTCATGACGCCAAAaccgcc                                                      >  2:422649/1‑149 (MQ=255)                                                                                                    tttACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAAGTTTCCATTTTTTCCCCCGG‑AAAAAAAACCCCGACGAACAACTGCAACACCACCACCTGTTTCAGGCTGATA‑CCCCATTTTCTGACGCCCAAgccgccgccgc                                                 >  2:793242/1‑151 (MQ=255)                                                                                                       aCGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGccgctgccgctg                                               <  2:624099/150‑1 (MQ=255)                                                                                                       aCGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGt                                              <  2:457819/151‑1 (MQ=255)                                                                                                       aCGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTTTTCCCCCGG‑AATAAAAACCCCCACCAACATCTGCAACACCACCCGCTGTTTCCGGCTGATA‑CCCCACTTCATTACCCCAAAacccccgccgc                                                 >  2:310390/1‑148 (MQ=255)                                                                                                        cGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑TAGGAGTTCATTTTTCCCCCCGG‑AATAAAAAACCCCCCGAACAACTTCCACACCCCCCGCGGGTTCACGCGGAAATCCCCATTT‑ATAACGCCCAAACCGCTGCCGCCGt                                              >  2:57482/1‑150 (MQ=255)                                                                                                           tGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTTCATTTTTCCCCCCGG‑AATAAACACCCCCACGGACATCTGCAACACCACCCGCTGTTTCAGGCTGATA‑CCCCCTTTCATTACGCCCAAGCCGCCGCCGCTGGAtt                                           >  2:800705/1‑150 (MQ=255)                                                                                                           tGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTTTCCCCACCG‑AATAAAAACCCCCACGAACATCTTCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAACCGCTGCCGCTGTAtt                                           >  1:555333/1‑150 (MQ=255)                                                                                                                 tCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTGTCCCCACGG‑AATAAAAACCCCGACGCACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCCAAACCGCTGCCCCTGTATTCaga                                       >  2:399545/1‑148 (MQ=255)                                                                                                                    ccAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCgg                                 <  2:705009/151‑1 (MQ=255)                                                                                                                    ccAGCGCCAGCAGCAATCCAACGGCGGTG‑AAGGTTTCCATTTGTCCCCCCGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGc                                   <  2:1117945/149‑1 (MQ=255)                                                                                                                          ccAGCAGCAGTCCACCGGCGGTG‑AAGGTTTTCATTTGTCCCCCCGG‑AATAAACACCCCGCCGCACATCTGCAACACCACCAGCTGTTTTCGGCTGATA‑CCCCCTTTCATGACGCCAAAGCCGCTGCCGCTGTATTTAGAGAGCGGGCCtt                            >  2:224359/1‑150 (MQ=255)                                                                                                                          ccAGCAGCAGTCCACCGGCGGTG‑AAGGGTTCCATTTGTCCCCACGG‑AATAAAAACCCCCACGAACATCTGCAAAACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAACCGCTGCCGCTGTATTCAGAGAGCGGGCCtt                            >  1:338784/1‑150 (MQ=255)                                                                                                                            agcagcagTCCACCGGCGGTG‑AAGGTTTTCATTTTTCCCCCCGG‑AATAAAAACCCCCCCGAACCTCTGCAACACCCCCAGCTGTTTCAGGCTGGTA‑CCCCCTTTTATTACCCCAAAACCGCTGCCGCTGTATTTAGAGAGCCGGCCTTcc                          >  1:478924/1‑150 (MQ=255)                                                                                                                             gcagcagTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTg                        <  2:986616/151‑1 (MQ=255)                                                                                                                               agcagTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCa                      <  1:214055/151‑1 (MQ=255)                                                                                                                                       aCCGGCGGTG‑AAGGGTTTCATTTTTTCCCCCGG‑AAAAAAAACCCCCACGGACATCTGCAACACCACCAGCTGTTTTTGGCTGATA‑ACCCCCTTCATGAAGCCAAAGCCGCTGCCGCCGTATTTAGAGAGCGGGGCCTCCTCCCGCTcccg               >  2:858308/1‑148 (MQ=255)                                                                                                                                          ggcggTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCg           <  1:646271/151‑1 (MQ=255)                                                                                                                                          ggcggTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCg           <  1:638603/151‑1 (MQ=255)                                                                                                                                           gcggTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCgg          <  2:1146119/151‑1 (MQ=255)                                                                                                                                                   aGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGc   <  1:437480/151‑1 (MQ=255)                                                                                                                                                     gTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGcc  >  2:241908/1‑150 (MQ=255)                                                                                                                                                     gTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGcc  >  2:950444/1‑150 (MQ=255)                                                                                                                                                      tttCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGcc  >  2:161403/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                       AAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTG‑AAGGTTTCCATTTGTCCCCACGG‑AATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATA‑CCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCC  >  NC_000913/2846526‑2846822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑