Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,177,531 | 0 | A | C | 42.4% | 105.3 / 10.3 | 59 | V127G (GTG→GGG) | nudF | ADP‑ribose pyrophosphatase |
Reads supporting (aligned to +/- strand): ref base A (25/9); new base C (0/25); total (25/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.19e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATC > NC_000913/3177384‑3177680 | gCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCg > 2:882530/1‑151 (MQ=255) ccTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCgg < 2:24211/151‑1 (MQ=255) tCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTgg > 1:399443/1‑151 (MQ=255) gCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGt < 1:1088772/151‑1 (MQ=255) tACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTg > 2:206835/1‑151 (MQ=255) aCCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTg < 1:888671/150‑1 (MQ=255) caTGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTAt < 2:254208/151‑1 (MQ=255) aaCGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGt > 1:1103581/1‑151 (MQ=255) gAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGc < 2:911057/151‑1 (MQ=255) atCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctct > 1:287318/1‑151 (MQ=255) tttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGc > 1:449103/1‑151 (MQ=255) tcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgc > 1:691383/1‑151 (MQ=255) ccacccAGCCCGAAAATCCCGCTTGCGGTCGGGGCGCCCATTCCCCCCCCAATAATTGACGAACGCTCCCTGGTGCCCCCCGGGCTTG‑CCAGAAAACTAAACCCGGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCt < 2:414086/143‑1 (MQ=255) cagccagcCCGTGAATACCGCTTGCGGTCGTGGCGCCCCCTCCCCCCACCATAATTGACGACCGCTCACTGGTGCCCCCCGGGCTTG‑CCAGAAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgcg < 1:498555/151‑1 (MQ=255) cagccagcCCGTAAAAACCGTTTGGGTGCGTGGCCCCCACTTCGCCAACATTAATTGACGACCGCTCACTGGTGCCCCCGGGGTTTG‑CCAGGAAACTAACCCCCGGTTTGGTCCGTTTGACTATCATTCCCGCCTCTTCAATCGCTTcgc < 1:888480/150‑1 (MQ=255) ccAGACGGGGAAACCCGCTTGCGGTCGTGGCGTCCCCTCCCCCCACCATAATGGACGAACGCTCACTGGTCCCCCCCGGGCTGG‑CCAGAAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGa < 2:753885/149‑1 (MQ=255) ccGTAAATCCCGTTGGGGGTCGTGGGGCCCCCTCCGCCCACCATAATTGCCGAACGCTCCCTGGTGCCCCCCGGGCTTG‑CCAGAAAACTTAACCCCGGTTTGGCCCGTTTGACTATCAGCCCCGCCTCTTCAATCGCTTCGCGACGGGCaa < 2:968520/151‑1 (MQ=255) aaTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATc > 1:790674/1‑130 (MQ=255) aaTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCt > 1:1116725/1‑151 (MQ=255) atcccGCTTGCGGTCGTGGCCTCCACTCCCCCCCCCATAATTGACGAACGCTCACTGGTCCCCCCCGGGCTTG‑CCAGAAAACTAAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCtt < 1:856778/148‑1 (MQ=255) ccGCTTGCGGTCGTGGCGTCCCCTCCCCCCACCATAATTGACGACCGCTCACTGGTGCCCCCCGGGTTTG‑CCAGGAAACTAAACCCCGGTTTGGTCCGTTTGCCTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTcc < 2:1007255/150‑1 (MQ=255) ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACt > 1:136675/1‑151 (MQ=255) ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACt > 2:959870/1‑151 (MQ=255) ttGCGGTCGTGGCGCCCCCTCCCCCCACCATAATTGACGACCGCTCACTGGTGCCCCCCGGGCTTG‑CCAGAAAACTAAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCcaca < 2:320678/149‑1 (MQ=255) tGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACtt > 2:354897/1‑151 (MQ=255) cGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCATTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCAccc > 1:1120205/1‑151 (MQ=255) gTGGCGCCCCCTTCCCCCCCCATAATTGACGACCGCTCACTGGTCCCCCCCGGGTTTG‑CCAGGAACCTTAACCCGGGTTGGGCCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCa < 1:830726/147‑1 (MQ=255) gTGGCGCCCACTCCCCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGTTTG‑CCAGGAAACTAAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCct < 1:779936/151‑1 (MQ=255) gTGGCCCCCACTCCCCCCACCATAATTGACGAACGCTCACGGGTCCCCCCCGGGCTTG‑CCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCct < 2:493100/151‑1 (MQ=255) ggctccccctcccccccccATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAATTTAACCCCGGTTTGGCCCTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCctc < 1:1063259/137‑1 (MQ=255) ccccTTCCCCCCCAATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGAAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCaa < 1:1147915/147‑1 (MQ=255) ttCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGATTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatca > 1:793730/1‑149 (MQ=255) ccccccccAAAATTGACGACCGCTCACTGGTCCCCCCCGGGCTTG‑CCAGAAAACTTAACCCCGGTTTGGTCCTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatcat < 2:364094/146‑1 (MQ=255) aTAATTGACAAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGAAAACTTACCCCCGTTTTGTTCCTTTTTACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCa < 1:460625/151‑1 (MQ=255) aaTTGACGAACGCTCACGGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCAt < 1:68130/150‑1 (MQ=255) gACGAACGCTCACTGGTCCCCCCCGGGCTTG‑CCAGGAATCTTACCCCGGGTTTGGTCGGTTTTACTACCGGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCa < 1:829856/141‑1 (MQ=255) aCGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTcc > 1:248279/1‑149 (MQ=255) gAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCTGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGt > 1:1011103/1‑150 (MQ=255) cccccACTGGTCCCCCCCGGCCTTG‑CCAGAAAACTTAACCCCGGTTTGTTCCTTTTGTCTACCAGCCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGcc < 2:1010557/147‑1 (MQ=255) tctgctgtccccctcGGCCTTG‑CGATGACACCTACCCCCGGTTTGGACCGTTTAACTACTCTTCCCGCCTCTTCAACCGCTTCGCGACGGGCAACATCTGCCACACTTTCCCCCTCTTCAATCAGCCCGGCAACCATCTCCAGTAGcct < 2:833497/140‑2 (MQ=255) tCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCa > 2:1064428/1‑149 (MQ=255) aCTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAggg > 1:1155102/1‑151 (MQ=255) ggTGCCCCCCGGGCTTC‑CCAGAAAACTTAACACCGTTTGGGCCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAgggg < 1:1162127/149‑1 (MQ=255) ggTCCCCCCCGGGCTTG‑CCAGGAACCTAAACCCCGGTTTGGCCCTTTTAACTATCAGCCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAgggg < 2:1170350/149‑1 (MQ=255) gCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCg > 1:268067/1‑151 (MQ=255) ccccccGGGCTTG‑CCAGAAAACTTAACCCCGTTTTGGCCCGTTGGCCTATAATCCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCg < 1:816916/150‑1 (MQ=255) cccccGGGGTTGCCCAGAAAACTAAACCCCGTTTTGGCCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGc < 2:421069/151‑1 (MQ=255) cccGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTgg > 2:564969/1‑151 (MQ=255) gggTTTG‑CCAGGAAACTTAACCCCGTTTGGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGgtgt < 1:960188/151‑1 (MQ=255) gggCTTG‑CAAGAAACCTTAACCCCGGTTTGGCCCGTTAGCCTTCCAGCCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGgtgt < 1:442583/151‑1 (MQ=255) ttG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGt > 1:1090693/1‑150 (MQ=255) ccAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTAcgc > 2:729494/1‑151 (MQ=255) gAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGc > 2:1051710/1‑150 (MQ=255) aaaCTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCa > 2:525375/1‑150 (MQ=255) aaaCTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCa > 1:928633/1‑150 (MQ=255) aaaCTTAACACCGGTTTGGTCCGTTTGACTAACAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGc < 1:212313/149‑1 (MQ=255) aaCTAAACCCCGGTTTGGTCCTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAAt < 2:997030/151‑1 (MQ=255) aaCCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATc < 1:206878/147‑1 (MQ=255) aaCCCCGGTTTGGCCCTTTTGATTCTCATTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCg < 2:918862/149‑1 (MQ=255) cacCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATc > 1:636603/1‑151 (MQ=255) | GCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTG‑CCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATC > NC_000913/3177384‑3177680 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |