Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	213,341	0	A	G	50.0%	67.6 / 25.1	66	G337G (GGA→GGG)	tilS	tRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (8/25);  new base G (33/0);  total (41/25)
Fisher's exact test for biased strand distribution p-value = 2.65e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCC  >  NC_000913/213194‑213490                                                                                                                                                    |                                                                                                                                                       tGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAg                                                                                                                                                      >  1:1008290/1‑150 (MQ=255)     tCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCGGGGGAgggg                                                                                                                                                   >  2:604508/1‑146 (MQ=255)       aCCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAgtg                                                                                                                                                    >  2:235615/1‑146 (MQ=255)          cTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTAAAGCtt                                                                                                                                            >  1:97125/1‑151 (MQ=255)               tACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAAttc                                                                                                                                       >  1:415312/1‑149 (MQ=255)               tACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATg                                                                                                                                        <  1:175047/150‑1 (MQ=255)                aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAAACCtttaat                                                                                                                                        >  2:1077355/1‑145 (MQ=255)                   tttGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATTCgggg                                                                                                                                   >  1:645719/1‑150 (MQ=255)                   tttGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCGGGGAAGGGTTCAGCTTAAAGCggg                                                                                                                                    >  1:488852/1‑150 (MQ=255)                          gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAAGGTAAAACTTAAATAGGGAgg                                                                                                                                 >  1:171416/1‑146 (MQ=255)                           cgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGAGGCGgta                                                                                                                            >  1:797763/1‑150 (MQ=255)                            gTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGGAGGGGAGAGCTTCAAGCgggggggg                                                                                                                               >  2:32743/1‑144 (MQ=255)                             tttGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGATTAAtttgggggggggtgtt                                                                                                                          >  1:668440/1‑135 (MQ=255)                             tttGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGGGGTAtt                                                                                                                          >  1:540077/1‑150 (MQ=255)                             tttGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTTATGCGGGGGGGGGTAttt                                                                                                                         >  2:681780/1‑150 (MQ=255)                                   aTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTAATGCGGGGGGCGATATTCGccc                                                                                                                     >  2:718135/1‑149 (MQ=255)                                     ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGGAAGCCTAAAGCGGGGGGGGATTTTTGCCCAcc                                                                                                                  >  2:150969/1‑150 (MQ=255)                                      cGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTAAAGCGGGGGGGGATATTCGCCCTcccc                                                                                                                >  1:625456/1‑149 (MQ=255)                                       gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGAAAGCCTTAAGCGGGAGGGGATATTTGcccccc                                                                                                                  >  1:1173235/1‑148 (MQ=255)                                          gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGAGGGGGACAGCGTAATGCGGGGGGGGAGAATTGCCCTCCGCCTg                                                                                                             >  2:711903/1‑150 (MQ=255)                                           cTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGAGGAAAACTTAATGCGGGGGGGGGTATTTGCCCTCCCCGTg                                                                                                             >  2:509086/1‑149 (MQ=255)                                             aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACAGGCGGGGCTGGGGAGGGTACAGCCTTATACGGGAGGCGATATTCGCCCTCCCCGTg                                                                                                             >  2:276110/1‑147 (MQ=255)                                               cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCCTAATTCGGGGGGGGGTATTTGCCCCCCCCGGGCAg                                                                                                          >  2:566184/1‑148 (MQ=255)                                               cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACg                                                                                                       <  1:526853/151‑1 (MQ=255)                                                aGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGGCAGCTTAAAGCGGGGGGGGAGATTCGCCCCCCGCGGGCAGACg                                                                                                       >  2:168366/1‑150 (MQ=255)                                                 gTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGaa                                                                                                     <  1:1057737/151‑1 (MQ=255)                                                          gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc                                                                                            <  1:755015/151‑1 (MQ=255)                                                                 aTTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑Gtt                                                                                    <  1:439207/151‑1 (MQ=255)                                                                   tAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑Gttt                                                                                   <  1:1081912/150‑1 (MQ=255)                                                                          gTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATTCGGGGGGCGGTATTCCCCCCCCCCGGGCAGACGAAACGGGCAGCGGGC‑GGTTCAAAacg                                                                            >  1:8667/1‑150 (MQ=255)                                                                             aCCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGGGGGGACAGCCTAAAGCGGGAGGGGGGATTTCCCCCCCCCCTGCAAACGGAGCGGGCAGCGTGG‑GTGTCCAAacgc                                                                           >  2:56008/1‑148 (MQ=255)                                                                              ccGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAgg                                                                       <  2:1142622/151‑1 (MQ=255)                                                                               cGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGGGTATAGATTAATTCGGGGGGGGGTATTTGCCCTCCCCGTGCAGACGAAACGGGCAGCGGGC‑GTTTCAAAGCGCCAgg                                                                       >  1:47918/1‑150 (MQ=255)                                                                                gggCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGCGGTATTCGCCCCCCGCGTGCAGACGAAGCGGGCAGCGGGC‑GTTTCAAAACGCCAgg                                                                       >  1:502132/1‑149 (MQ=255)                                                                                   cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAATACGGGGGGCGATATTTGCCCTCCGCGTGCAGACGAAGAGGGCAGCGGGC‑GGTTCAAAACGCCCGGAtt                                                                    >  1:723448/1‑149 (MQ=255)                                                                                    aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCCTAATTCGGGGGGCGGTATTTTCCCCCCGCGTGCAGAAGAAAGGGGCAGCGGGC‑GGTTCCAAACGCCAGGaat                                                                    >  2:672815/1‑146 (MQ=255)                                                                                        cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCat                                                             <  1:1077688/151‑1 (MQ=255)                                                                                          aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAAAGCGGGGGGCGATATTTGCCCCCCCCGTGCAGACGAAGCGGGCAGCGGGC‑GGTTCAAAACGCCCGGGTTGCTGCatat                                                           >  1:956573/1‑151 (MQ=255)                                                                                          aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCatat                                                           <  2:1115520/151‑1 (MQ=255)                                                                                            aaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGGGGACAGCTTAATGAGGGGGGGGGTATTCGCCCCCCCCGGGCAGGCGAAGAGGGCAGCGGGG‑GGTTTAAAACGCCAGGGTGGCTGCatat                                                           >  2:958044/1‑149 (MQ=255)                                                                                                 tgtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGGTTTATGCGGGGGGGGATATTTGCCCCCCGCGTGCCGACGAAGCGGGCAGCGGGC‑GGTTCAAAACGCCCGGATTGCTGGATATTTTCggg                                                    >  2:1129603/1‑151 (MQ=255)                                                                                                  gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGc                                                   <  1:106356/151‑1 (MQ=255)                                                                                                  gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGc                                                   <  2:948987/151‑1 (MQ=255)                                                                                                     ccGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTa                                                <  1:3316/151‑1 (MQ=255)                                                                                                       gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGAAAGCTTAAATCGGGGGGCGGTATTCTCCCCCCCCCTGCAGACGAAACGGGCCGGGTGCGGTTTCAAA‑CGCCCGGGATGGTGCCTATTTTCggg                                                    >  2:743365/1‑145 (MQ=255)                                                                                                        tGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACCACCTAATGTGGGAGGGGATATTTGCCCTCCCCGGGGAGAAGAAGCGGGGAGCGTGG‑GGTTTAAAGAGCCAGGGTTGCTGCCTATTGTCGGGCGGaa                                               >  2:502008/1‑149 (MQ=255)                                                                                                          gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCTGGAAGGGGACAGCTTAATTCGGGAGGGGGTATTTGCCCTCCCCGGGCAGGCAAAGGGGGTCGGGGGC‑GGTTCAAAACGCCCGGGGTGGTGCattgttttgggggga                                                >  1:961242/1‑133 (MQ=255)                                                                                                           cAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggcg                                          <  2:625271/151‑1 (MQ=255)                                                                                                           cAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggcg                                          <  2:82629/151‑1 (MQ=255)                                                                                                             gACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGa                                        <  1:118400/151‑1 (MQ=255)                                                                                                                  ggCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTaa                                   <  1:287690/151‑1 (MQ=255)                                                                                                                  ggCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTaa                                   <  2:488709/151‑1 (MQ=255)                                                                                                                    cTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGCGGTATTTGCCCCCCGCGTGCAGACGAAACGGGCAGCGGGC‑GGTTCAAAACGCCAGGGTTGCTGCATATTGTTGGGGGGAACGGCGGGCGGaa                                   >  1:349668/1‑149 (MQ=255)                                                                                                                    cTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTa                                    <  2:554367/148‑1 (MQ=255)                                                                                                                      tCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCt                                <  2:1007966/150‑1 (MQ=255)                                                                                                                       cAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTaa                              <  1:32751/151‑1 (MQ=255)                                                                                                                        aaCCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACAACTTTATGCGGGGGGGGGAATTTTCCCCCCCCCCGCAGGCGAAGCGGGCAGAGCGG‑GGTTTAAAACGGCAGGAATGCTGCATATTGTCTGGGGTAAcagcgggaggcaaataa                              >  2:823107/1‑139 (MQ=255)                                                                                                                           cGCTGGAATTACCGGCGGGGCCGGGGAGTGGACAGCTTTATTCGGGGGGGGGTAATCCCCCTCCGCCGGCAGGCGAAGAGGGGAACGTGG‑GGTTCAAAACGCCAGGATTGCTTCATATTGTTGGGCGGAAcggcgggacggagctta                              >  2:341644/1‑136 (MQ=255)                                                                                                                               ggAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAAt                       <  2:97103/150‑1 (MQ=255)                                                                                                                                     aCCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCa                 <  1:489982/150‑1 (MQ=255)                                                                                                                                          cggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGc            >  2:766498/1‑150 (MQ=255)                                                                                                                                           ggggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCt           <  2:654835/150‑1 (MQ=255)                                                                                                                                           ggggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCt           <  1:115766/150‑1 (MQ=255)                                                                                                                                                tGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCg      >  2:192260/1‑150 (MQ=255)                                                                                                                                                  ggAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgc   <  2:1106963/151‑1 (MQ=255)                                                                                                                                                   gAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgcc  >  2:840361/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                       TGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCC  >  NC_000913/213194‑213490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑