Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,640,981	0	C	T	48.2%	46.0 / 35.6	56	F40F (TTC→TTT)	dtpB	dipeptide and tripeptide permease B
Reads supporting (aligned to +/- strand):  ref base C (3/26);  new base T (27/0);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 5.54e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTT  >  NC_000913/3640832‑3641128                                                                                                                                                      |                                                                                                                                                        tttCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttc                                                                                                                                                         <  2:1062826/150‑1 (MQ=255) tttCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttc                                                                                                                                                         <  1:642444/150‑1 (MQ=255)    cAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGtttt‑ttt                                                                                                                                                     >  2:195616/1‑149 (MQ=255)     aGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTttc‑tt                                                                                                                                                      >  1:850997/1‑148 (MQ=255)        aTGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTG‑ttt                                                                                                                                                >  1:1047161/1‑149 (MQ=255)             aTTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGtttt‑tttttttaa                                                                                                                                               >  2:847777/1‑140 (MQ=255)               tGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑t                                                                                                                                        <  1:440916/150‑1 (MQ=255)                ggAATGTTAATAAATATGAATACAACAACACCCATGGGTATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑tt                                                                                                                                       <  1:772494/150‑1 (MQ=255)                ggAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTtttt‑tttttttaaaaacttg                                                                                                                                        >  2:431403/1‑137 (MQ=255)                 gAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTgg                                                                                                                                     <  2:367096/151‑1 (MQ=255)                         ataaataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTG‑TTAAAAAGC‑TTTGAtttt                                                                                                                                >  1:916949/1‑146 (MQ=255)                          taaataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGATTTT‑TTTT‑TTTAAAATC‑TTGGCtt                                                                                                                                  >  2:967765/1‑145 (MQ=255)                           aaataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCaa                                                                                                                           <  1:235211/151‑1 (MQ=255)                           aaataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCa                                                                                                                            <  2:692147/150‑1 (MQ=255)                            aataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTT‑TTTT‑TTTAAAAGC‑TTGGGTTTTCGCaa                                                                                                                           >  2:568255/1‑150 (MQ=255)                            aataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTT‑TTCG‑TTTAACAGC‑TTGGGTTTTTGCaa                                                                                                                           >  1:699470/1‑150 (MQ=255)                             atatGAATACAACAACACCCATGGGGATGCTGTAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAaga                                                                                                                         <  2:401919/151‑1 (MQ=255)                              tatGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTG‑TTAAACAAC‑TTGGGTTCTCGcca                                                                                                                           >  1:642543/1‑146 (MQ=255)                                tGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTG‑TTTAAAAGC‑CTGGGTTCTCGCAagag                                                                                                                        >  1:1102659/1‑149 (MQ=255)                                  aaTACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAgg                                                                                                                    <  1:17399/151‑1 (MQ=255)                                   aTACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTAGGCTACTACGGCGTGCAGGGCGTAATGGCGGTTTTT‑TTCT‑TTAAAAAGC‑TTAGGTTATCGCGAGAGCCgg                                                                                                                    >  1:395188/1‑150 (MQ=255)                                      caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGTGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCtt                                                                                                                 <  2:736829/150‑1 (MQ=255)                                       aacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTT‑TTCG‑TTTAAAAAT‑GTTGATTCTCGCCAGAGGAGGGtttt                                                                                                               >  2:1153251/1‑151 (MQ=255)                                         caacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTCTTTCC‑TTAAA‑AAC‑TTGGATTCTCGCCACAGAAGGCgttttt                                                                                                             >  2:597764/1‑149 (MQ=255)                                             acCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTG‑TTAAACAAC‑TTGGGTTTTCGCAAGAGCAGGCtttttt                                                                                                             >  1:319989/1‑145 (MQ=255)                                               ccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTCGTTTAAAAACC‑TTGG‑TTCTCGCCAGAGCAGGGTTTTTTTACttt                                                                                                       >  1:754271/1‑151 (MQ=255)                                               ccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTT‑TTTG‑TTTAAAAGC‑TTTGGTTTTCGCAAGAGCAGGGGTTTTTCACttt                                                                                                       >  1:386616/1‑151 (MQ=255)                                                 aTGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTCG‑TTAAAAAGC‑CTGGAATCTCGCAAGAGAAGGCttttttttccttt                                                                                                      >  1:986983/1‑141 (MQ=255)                                                 aTGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGATTTT‑TTTG‑TTAAAAACG‑CTGCGTTTTCGCAAGAGCAGGCTTTTTTTGCttttt                                                                                                     >  1:231754/1‑150 (MQ=255)                                                     ggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGATTTT‑TTCG‑TTTAAAAGA‑CTGGATTTTTGCCAAAACAGGGTTTTGTGACTTTTTGt                                                                                                   >  2:1086563/1‑149 (MQ=255)                                                                caACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTT‑TTTT‑TTTAACAGC‑TTGGGTTTTCGCAAAAGCAGGCGTTTTTTACTTTTTGGGCtttt                                                                                             >  2:148649/1‑144 (MQ=255)                                                                   ccTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTG‑TTTAACAAC‑TTTGATTCTCGCCAGAGCAGGGTTTTTTCACTTTTTGTGCTTTttct                                                                                          >  1:439435/1‑144 (MQ=255)                                                                     tCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTG‑TTTAAAAGC‑TTTGGTTTTCGCAAGAGCAGGCGTTTTTTTCTTTTTGTGCTTTTTTTGCGCTgg                                                                                   >  2:437613/1‑149 (MQ=255)                                                                      cGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTG‑TTTAACAGCTTTGGATT‑TCCCAAAAGGAGGCTTTTTTTCCTTTTTGGGCtttt                                                                                             >  2:378091/1‑138 (MQ=255)                                                                      cGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTG‑TTTAACAGC‑TTGGGTTTTCGCAAGAGCCGGCTTTTGTTACTTTTTGTGCtttt                                                                                             >  2:87149/1‑138 (MQ=255)                                                                      cGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGt                                                                                  <  2:211922/149‑1 (MQ=255)                                                                      cGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTc                                                                                 <  2:916654/150‑1 (MQ=255)                                                                      cGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCt                                                                                <  2:959783/151‑1 (MQ=255)                                                                      cGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCt                                                                                <  2:218901/151‑1 (MQ=255)                                                                                tCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCa                                                                      <  1:1084060/150‑1 (MQ=255)                                                                                      tCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTCG‑TTTAAAAGC‑TTGGATTTTCGCCAGAGCAGGCTTTTTTTTCTTTTTGTGCTTTTTCTCCGCTTGGCTA‑TTGCCTCCttt                                                                   >  2:246124/1‑147 (MQ=255)                                                                                         tttttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTcc                                                                 <  2:774111/146‑1 (MQ=255)                                                                                         tttttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCa                                                                <  2:66124/147‑1 (MQ=255)                                                                                               tCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTG‑TTTAAAAGC‑TTGGGTTCTTGCCAGAGCAGGCTTTTTTTACTTTTTGTGCTTTTTCTTCGCTGGGCTT‑TTGCCCCCttttcctttgggggtt                                                      >  2:1162029/1‑138 (MQ=255)                                                                                                cGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTa                                                      <  1:94155/150‑1 (MQ=255)                                                                                                cGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTT‑TTTG‑TTTAAAAGC‑CTGGGTTCTCGCAAGAGCAGGGTTTTTTCACTTTTTGTGCTTTTTTTGCGCCGGGCTA‑TTGCCTCAttttcctt                                                              >  1:59574/1‑137 (MQ=255)                                                                                                    ttATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTG‑TTTAAAAAC‑TTGGATTTTCGCAAGAGGAGGCTTTTTTGCCTTTTTGTGGCTTTTTTTCGCTGGTCTA‑TTGCCCCCTTTTCATTtgcggggttgt                                                   >  1:1067899/1‑143 (MQ=255)                                                                                                    ttATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTT‑TTCG‑GTTAAAAGC‑TTGGGTTTTCCCCAGAGCAGGGCTTTTTTACTTTTTGGGCTTTTTCTGCGCTGGGCTATTGGCTTATTTTCCATTggggggat                                                      >  1:1147863/1‑144 (MQ=255)                                                                                                     tATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTT‑TTTGTTTAAAAACC‑TTGG‑TTTTCGCCAAAGGCGGGTTTTTTTACTTTTTGGTTTTTTTTTGTGCTGGGCTT‑TGGGCTCATTTTCATTtgcgggctt                                                     >  2:155895/1‑142 (MQ=255)                                                                                                      aTGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCgg                                                <  1:431524/150‑1 (MQ=255)                                                                                                      aTGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGc                                               <  2:900047/151‑1 (MQ=255)                                                                                                       tGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTT‑TTTG‑TTTAAAAGC‑TTTGGTTTTCGCCAGAGCCGGGTTTTTTTACTTTTTGTGCTTTTTTTTTGCTGGGCTA‑TTGGCTCATTTTCATTTGGGGCTATGTTGGCg                                              >  1:612403/1‑151 (MQ=255)                                                                                                                   ggCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGc                                               <  1:1036987/138‑1 (MQ=255)                                                                                                                   ggCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTgg                                  <  2:374463/151‑1 (MQ=255)                                                                                                                       actacGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGa                               <  2:591080/150‑1 (MQ=255)                                                                                                                            ggCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAAc                         <  2:375125/151‑1 (MQ=255)                                                                                                                               gTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCa                      <  1:848044/151‑1 (MQ=255)                                                                                                                                             ggCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTATTgg         <  1:1027225/150‑1 (MQ=255)                                                                                                                                               cGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGc      <  1:597941/151‑1 (MQ=255)                                                                                                                                                ggTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCa     <  1:1086896/151‑1 (MQ=255)                                                                                                                                                agTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGc      >  2:221496/2‑150 (MQ=255)                                                                                                                                                 gTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCAc    >  1:879535/1‑151 (MQ=255)                                                                                                                                                    ttc‑ttcG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACtt  >  2:885442/1‑150 (MQ=255)                                                                                                                                                     tc‑ttcG‑TTAATCAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCTCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACtt  <  2:395086/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                        TTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTC‑TTCG‑TTAAACAGC‑TTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTA‑TGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTT  >  NC_000913/3640832‑3641128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑