Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	46.8%	121.6 / 23.1	79	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (5/37);  new base G (37/0);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 3.08e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCG  >  NC_000913/354141‑354433                                                                                                                                                     |                                                                                                                                                  ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTg                                                                                                                                                  <  2:621706/149‑1 (MQ=255)  gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGaaa                                                                                                                                               <  1:328609/151‑1 (MQ=255)    gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGa                                                                                                                                             <  2:564901/151‑1 (MQ=255)      aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATg                                                                                                                                           <  1:800981/151‑1 (MQ=255)       tCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGaaa                                                                                                                                               >  1:908254/1‑146 (MQ=255)        ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGaa                                                                                                                                                >  1:16830/1‑145 (MQ=255)         cGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGATgcg                                                                                                                                         >  1:686150/1‑150 (MQ=255)          gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCt                                                                                                                                       <  2:512883/151‑1 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGGTgcgc                                                                                                                                        >  2:792417/1‑148 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTg                                                                                                                                      >  1:345351/1‑150 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAggagg                                                                                                                                          >  2:372067/1‑142 (MQ=255)                  gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAggg                                                                                                                                <  1:635259/150‑1 (MQ=255)                   aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGATGCGCTGAAAggg                                                                                                                                >  2:908871/1‑149 (MQ=255)                    cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAggg                                                                                                                                >  1:185528/1‑148 (MQ=255)                     ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAgg                                                                                                                            <  1:203496/151‑1 (MQ=255)                         aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtgg                                                                                                                         <  1:529660/150‑1 (MQ=255)                               ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtgg                                                                                                                   <  2:420267/150‑1 (MQ=255)                               ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                    <  1:437191/149‑1 (MQ=255)                                tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGGGCCGGAAgggggggggggggggggg                                                                                                                 >  2:65659/1‑136 (MQ=255)                                 aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGt                                                                                                                <  2:185488/151‑1 (MQ=255)                                     tcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGAAGCGCTGGAAGGGCAggggggggggggggtt                                                                                                              >  2:789159/1‑144 (MQ=255)                                         gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCAtt                                                                                                        <  1:809298/151‑1 (MQ=255)                                          cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGTGAAAGAAGCGCTGAAAGGGCAGGTGGGGGGGGGGGTTGTCAtt                                                                                                        >  1:432525/1‑150 (MQ=255)                                               tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGAAGCGCTGGAAGGGCAGGGGGGGGGGGGGGTTGTCAtt                                                                                                        >  2:235327/1‑145 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAA‑ATGCGCTGAAAGGGCAGGTGGGGGGGGGGGTTTTCATTCCGaaa                                                                                                  >  1:667808/1‑150 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaaa                                                                                                  <  2:453339/150‑1 (MQ=255)                                                 aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGAAAGATGGGGTGGAAGGGGAGGTGGGGGGGGGGTTTGTCATTCCgga                                                                                                   >  2:1169507/1‑146 (MQ=255)                                                  cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGGTTGTCATTCCGaaa                                                                                                  >  1:473032/1‑148 (MQ=255)                                                    aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGTTTGTCATTTCGaaa                                                                                                  >  1:1017391/1‑146 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                            <  2:323249/150‑1 (MQ=255)                                                         aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                        <  1:395256/151‑1 (MQ=255)                                                         aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                        <  2:938357/151‑1 (MQ=255)                                                          ttAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGAAGCGCCGAAAAGGGAGGGGGCGGGGGGGGTTGTCATTCCGAAAAAGAGCg                                                                                           >  2:281566/1‑147 (MQ=255)                                                              cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTTGGGGGAAAGATGCGCTGGAAGGGCAGGTGGCGGGGGCGTTTGTCATTTCGAAAAAGAGCGGCAg                                                                                       >  1:110495/1‑147 (MQ=255)                                                                  gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                <  2:186018/150‑1 (MQ=255)                                                                     gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGTGGGGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                  >  1:437223/1‑145 (MQ=255)                                                                         aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTg                                                                         <  2:345268/150‑1 (MQ=255)                                                                         aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGa                                                                        <  1:1056818/151‑1 (MQ=255)                                                                           cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg                                                                      <  1:896946/151‑1 (MQ=255)                                                                           cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAAAGAGCGACAGTCTGGAAGATCGTg                                                                         >  2:1154231/1‑148 (MQ=255)                                                                            ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGAAGCGCTGAAAGGGCAGGGGGGGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg                                                                      >  1:666143/1‑150 (MQ=255)                                                                             gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                    <  1:77934/151‑1 (MQ=255)                                                                                ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                 <  1:963695/151‑1 (MQ=255)                                                                                 gggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcgc                                                                <  2:1017065/151‑1 (MQ=255)                                                                                    aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACt                                                             <  1:459675/151‑1 (MQ=255)                                                                                    aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTTCGAAAGAGAGGGACAGGCTGGGAGATCGGGGTGGGGGGCAc                                                              >  2:254014/1‑150 (MQ=255)                                                                                     cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTTGGGGGAAAGATGCGGTTGAAGGGGAGGAGGGGGGGGGGGTTGTTATTTCGAAAGAGGGCGACAGGCTGGAAGACCGTGGTGTGGGGCACt                                                             >  1:750875/1‑150 (MQ=255)                                                                                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAAAAGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTCCTTCCGGAAGAGAGCGAACGGCTGGGAGATTGTGGTGGGGCGCACTc                                                            >  2:951486/1‑150 (MQ=255)                                                                                       cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc                                                          >  1:562744/1‑151 (MQ=255)                                                                                         cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCaa                                                        <  1:617477/151‑1 (MQ=255)                                                                                         cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGAAAGAGAGGGAGAGGCTGGGAGATCGTGATGTGGCGCACTCGc                                                          >  2:893106/1‑149 (MQ=255)                                                                                          gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCaa                                                        <  2:759016/150‑1 (MQ=255)                                                                                           agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGGAAAGGCAGGTGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGGGCACTCGc                                                          >  2:223330/1‑147 (MQ=255)                                                                                            gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                     <  2:385028/151‑1 (MQ=255)                                                                                            gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                     <  1:366696/151‑1 (MQ=255)                                                                                            gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGA‑GGGGGGGGGGAAAGAGGGGCCGAAAGGGCAGGTGGGGGGGGCGGTTTTCCTTTCGGAAGAGGGCGGAAGTCTGGGAGAAAGGGGGGTGGCGCAc                                                              >  2:252352/1‑142 (MQ=255)                                                                                             aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGGGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTCAATTCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGGGGAGTCGCaa                                                        >  2:316545/1‑147 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGGTAGATTCGCTGAAAGGGAAGGTGGCGGTGGGGTTTTTCCTGCCGGAAGAGAACGACAATCAGGAAAATCGGGATGTGGGGGACTCCCAaaaggaag                                                 >  1:1129533/1‑146 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                <  1:685476/151‑1 (MQ=255)                                                                                                  agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGGGCTGAAAGGGCCGGGGGCGGGGGCGTTTGTCCTTCCGAAAGAGAGCGACCGGCTCGGAGACAGGGATGTGGCGCACTccccaaaaaaaggg                                               >  2:430418/1‑138 (MQ=255)                                                                                                      agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                 >  1:683000/1‑145 (MQ=255)                                                                                                      agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGTTGGGGTGGAAGAAGCGCTGAAAAGGGGGGTGGCGGGGGGGCTTGTCATTCCCAAAGAGGGAGACAAGATGGAAGATCGTGGGGTGGCGAAccgcacagggaa                                                   >  2:919238/1‑132 (MQ=255)                                                                                                         aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                         <  2:1141341/150‑1 (MQ=255)                                                                                                         aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGTAAGATGCGCTGAAAGGGCAGGTGGCGGGGGGGGTTGTCATTCCGGAAGAGAGCGACAGTGTGGAAGAACGGGATGTGGGGCACTCGCCAGAGAAGGGGGATATgg                                        >  2:247303/1‑151 (MQ=255)                                                                                                           ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCCTTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAAGCGATTATGgc                                       >  1:220769/1‑150 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                        <  2:773127/147‑1 (MQ=255)                                                                                                              cAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtg                                   <  1:856585/151‑1 (MQ=255)                                                                                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCTGCAAGGGCAAGGGGGGGGGGGGGTTTTTATTTCGAAAGAGAGCCGCAGTCTGGAAAATCGCGATGTGGGGCACCCGCAAGAGGAGGGGATTATTgcgccg                                   >  1:542566/1‑148 (MQ=255)                                                                                                                 ccATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtg                                <  2:915645/151‑1 (MQ=255)                                                                                                                    tCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAAGGCAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggggg                               >  1:647959/1‑149 (MQ=255)                                                                                                                     ccGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGAAGCGCTGGAAGGGCAGGGGGCGGGGGCGGTTGTTCTTCCCAAAGAGAGGGACAGTCTGGAAGAGCGGGATGTGGGGCACTCGCAAGAGAAGGCGGGTATGGCGCtggggggc                             >  2:555668/1‑148 (MQ=255)                                                                                                                      cGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAg                           <  2:867866/151‑1 (MQ=255)                                                                                                                       gggCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAAATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                            >  1:1030257/1‑149 (MQ=255)                                                                                                                          cGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGGGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGGGATTATGGCGCCGGTGGGCAGcccg                       >  1:1059697/1‑149 (MQ=255)                                                                                                                              gCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg                   <  1:907000/151‑1 (MQ=255)                                                                                                                                        cGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg         <  2:220724/151‑1 (MQ=255)                                                                                                                                        cGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg         >  1:1095275/1‑151 (MQ=255)                                                                                                                                              gtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgcc    >  1:392591/1‑151 (MQ=255)                                                                                                                                                 ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg  <  1:416828/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCG  >  NC_000913/354141‑354433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑