Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,363,421	0	T	C	44.2%	50.7 / 10.9	43	G71G (GGA→GGG)	yfaY	inactive PncC family protein
Reads supporting (aligned to +/- strand):  ref base T (18/6);  new base C (0/19);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 2.28e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGG  >  NC_000913/2363275‑2363563                                                                                                                                                    |                                                                                                                                                tCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑ccc                                                                                                                                             >  1:401161/1‑150 (MQ=255)           tACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGtt                                                                                                                                  >  2:236497/1‑151 (MQ=255)            aCGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGtt                                                                                                                                  >  1:868308/1‑150 (MQ=255)             cGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTa                                                                                                                                 >  1:648956/1‑150 (MQ=255)                          ccATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATc                                                                                                                   >  1:37266/1‑151 (MQ=255)                             ttccTCGTCCACGTTCGTGAAAATGGCGTTCCTTCTTTTGGAGCCGGGCTTCATGCACCACCAGCCCTCCACTTTTTGCTGTCGCGGCGGCGAGTGCGTTTAAAT‑CATCGCTGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATc                                                                                                                   <  1:404492/145‑1 (MQ=255)                                cccGTCCACGTTCGTGAAAATAGCTTTCCATCTTTTTGAGCCAGGCTTCATGCACCACCAGGCCTTCCCCTTTTGCTGTCGCGGGGGCGAGGGCGATAAAAT‑CACCGCTGGCCGGC‑CCCGGCCCGCCGTTAACGATCAGCACATCGGCGTg                                                                                                             <  2:678404/149‑1 (MQ=255)                                  cGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCt                                                                                                           >  2:147019/1‑151 (MQ=255)                                          ttCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGt                                                                                                   >  2:54300/1‑151 (MQ=255)                                             gggaaaaTAGCGTCCCTTCTTTGGGGGCCAGGTTTCAGCCAGCCCCGGCCCTTCCCTTTTTGCTGTCGCGGGGGCGGGTGCGCTAAAAC‑CGCCGGTGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTa                                                                                                      <  1:12967/141‑1 (MQ=255)                                             gTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTc                                                                                                 >  2:615605/1‑150 (MQ=255)                                               ggAAATAGCTTTCCATCTCTTGGAGCCAGGCTTCATGCACCACCAGGCCTTCCCCTTTTGCTGTCGCGGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTc                                                                                                 <  2:816018/148‑1 (MQ=255)                                                 aaaTAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCACCCCCAGCCCTTCACTTTTTGCTGTCGCAGCGGCGAGTGCGTTTAAAT‑CATCGCTGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTcgcg                                                                                              <  2:541886/149‑1 (MQ=255)                                                  aatggcttcccATCTTTTGGCGCCGGGCTTCATGCACCACCGGCCCTCCCCCTTGTGCGGCGGCGGGGGGGGGTCCGTTAAAAT‑CACCGCGGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCgaag                                                                                           <  1:308415/142‑1 (MQ=255)                                                  aatggcttcccACCTCTTTGGCCCAGTTTCCACCCAGCCCCGGGCCTCCCCCTTTGGCTGTCGCGGGGGCGTGTGTTTTTAATT‑CACCGCGGGCCGTC‑CCCACCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCgaag                                                                                           <  1:336320/142‑1 (MQ=255)                                                        gTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATg                                                                                       >  1:707652/1‑149 (MQ=255)                                                             aTCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGAc                                                                                  >  2:753614/1‑149 (MQ=255)                                                              tctcTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTa                                                                                >  1:763048/1‑150 (MQ=255)                                                                      aGCAAGGTTCCACGCAGCACCAGCCCTTCCCCTTTTGCTGTCACGGCGGCGAGTGCCCTTAAAT‑CATCGCGGGTCGGC‑CCCGGACCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTa                                                                                <  2:649291/142‑1 (MQ=255)                                                                       ccgatctttCAGGCACCCCCAGGCCTTCCCTTTTTGCTGCCGCAGCGGCGAGTGCGCTAAAAT‑CATCGCTGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGtcatca                                                                        <  1:314425/142‑1 (MQ=255)                                                                        cctgccTTCACGCAGCCCCAGTCCTTCACCTTTTCCTGGCCCAGCGGCGAGGGCGCTAAAAT‑CCTCGCCGTCCGTC‑CCCTGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGtc                                                                            <  1:671149/139‑1 (MQ=255)                                                                              ttaaTGCACCCCCAGCCCTTCCCTTTTGGCTGTCGCGGCGGGGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTAt                                                                 <  2:320487/146‑1 (MQ=255)                                                                              ttCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGtcatca                                                                        >  2:314472/1‑142 (MQ=255)                                                                              ttCATGCAGCACCAGCCCTTCCCTTTTTGCTGCCGCAGCGGCGAGGGCGCTAAAAT‑CATCGCTGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTa                                                                  <  1:436648/148‑1 (MQ=255)                                                                              ttCAGGCAGCACCAGGCCTTCCCCTTTTGCGGTCGCGGGGGCGGGTGCGCTTAAAT‑CTGCGCGGGTGGGT‑CCCACCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTAt                                                                 <  2:487584/149‑1 (MQ=255)                                                                                       ccccAGGCCTTCCCTTTTTGCTGTCGCGGGGGAGATTCCGTTTAACC‑CTTCGCGGGTCGGC‑CCCACCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTAt                                                                 <  1:92617/138‑1 (MQ=255)                                                                                       acccggcccTCCACTTTGTGCTTGCGCAGCGGCGAGTGCGATAAATC‑CTTCGCGGTTGGCT‑CCAAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGt                                                       <  2:130744/143‑1 (MQ=255)                                                                                         ccAGCCCTCCCCTTTTGGCTGCCGCGGGGGGGAGTGCCATAAAAT‑CCCCGGGGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTAtt                                                    <  1:259189/151‑1 (MQ=255)                                                                                              ccTTCCCTTTTTGCTGCCGCAGGGGCGAGTGCGCTAAAAT‑CACCGCTGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTAtt                                                    <  2:486361/146‑1 (MQ=255)                                                                                               cTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTgcggcg                                              >  1:2347/1‑151 (MQ=255)                                                                                                ttCCCCTTTTGCTGTCGCGGCGGCGAGTGCTCTTAAAC‑CATCGCTGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTgc                                                  <  2:754436/146‑1 (MQ=255)                                                                                                 tCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTgcggcg                                              >  1:155954/1‑149 (MQ=255)                                                                                                      ttttGCTGTCGCGGGGGCGAGTGCCCTAAAAT‑CATCGC‑GGTCCGTCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCgaga                                           <  1:194773/147‑1 (MQ=255)                                                                                                       tttGCTGTCGCGGCGGGGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAAt                                       <  2:380470/150‑1 (MQ=255)                                                                                                         tGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGGCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATgg                                     >  1:377944/1‑150 (MQ=255)                                                                                                                  aGCGGCGAGTGGGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTTACGATCAGCACATCGGCGTGGTGACTACCTTTGCGAAAAATGGTGACTAAGGCATTAAGGGTATTCCCCACCGGATTGGGGCGAGATAAATGCAACCCCtg                            >  1:456843/1‑150 (MQ=255)                                                                                                                            tgcgttAAATCCACCGCTGCTCGCC‑CCCCCCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATgaaag                     <  1:77827/144‑1 (MQ=255)                                                                                                                             cgtttAAAT‑CATCGGTGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGaaaaa                <  2:189655/148‑1 (MQ=255)                                                                                                                             cgcTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGaaaa                 >  2:126506/1‑150 (MQ=255)                                                                                                                                 tAACT‑CATCGCTGGTCGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATc             <  2:734965/150‑1 (MQ=255)                                                                                                                                   aaaacctcGCGGGTGGGC‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATgaaagaa                   <  1:826939/137‑1 (MQ=255)                                                                                                                                       catcGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGgcca        <  2:150431/150‑1 (MQ=255)                                                                                                                                           gcgggccggc‑cCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGgccagcca    <  1:648591/144‑1 (MQ=255)                                                                                                                                            cTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAgg  >  2:23492/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                TCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAAT‑CATCGCTGGTCGGT‑CCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGG  >  NC_000913/2363275‑2363563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑