Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,921,173	0	T	C	42.1%	50.1 / 11.2	38	I310V (ATT→GTT)	gudP	putative D‑glucarate transporter
Reads supporting (aligned to +/- strand):  ref base T (17/5);  new base C (0/16);  total (17/21)
Fisher's exact test for biased strand distribution p-value = 1.04e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGAC  >  NC_000913/2921024‑2921322                                                                                                                                                        |                                                                                                                                                      ggCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATc                                                                                                                                                      <  2:708388/151‑1 (MQ=255)            tAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGcac                                                                                                                                           >  2:55163/1‑151 (MQ=255)            tAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAAAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGcac                                                                                                                                           >  2:247716/1‑151 (MQ=255)             aaaGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGcac                                                                                                                                           >  2:530706/1‑150 (MQ=255)              aaGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCacac                                                                                                                                         <  2:824607/151‑1 (MQ=255)              aaGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCacac                                                                                                                                         <  2:776361/151‑1 (MQ=255)               aGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACAcc                                                                                                                                        >  1:196750/1‑151 (MQ=255)                          atcCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAaaac                                                                                                                             >  2:130058/1‑151 (MQ=255)                          acccACCCAACGTTGCCGTGGTTGCAGACCCCCACCACCATCGCCAGCACCATGCCCATTACGATCGGTGTTTTCCGCGCAATGTTCAGCGATCCGGTGCGGCGCATA‑AGCCAG‑TCGGAAATAACCCCCCCCAGCACACCGCCGATaaaa                                                                                                                              <  2:461296/148‑1 (MQ=255)                               ctaaaCGTTGACGTGGTTGCAGACCACCACCACCATCGACGGCAACATCCCCATTACGATCGGTGTTTTCCGCGCAATGTCAAGCGATCCCGGGCGGCGAAAC‑CCCCAG‑TCGGAAATAACCCCCCCCACCACACCGCCGATAaaaccgc                                                                                                                          <  1:365269/146‑1 (MQ=255)                                   aCGTGGACGTAGTTGCAGAACCCCATCCCCATCGCCAGCAACATGCCAATTACGATCGGTTTTTTCCGCGCATTTTTCAGCGATCCCGTGCGGCGCACC‑AGCCAG‑TCGAAAATAACCCCCCCCAGCACACCGCCGATAaaaccgcaaaccg                                                                                                                    <  2:720789/151‑1 (MQ=255)                                    cGTTGACGTAGTGCCAGAACACCATCCCCACCGACACCAACATGCCCATTCCGCTCGTTGTTTTCCGCCCAATGTTCAGCGATCCCGTGCGGCGCACC‑ACCCAG‑TCGAAAATAACCCCCCCCAGCACACCGCCGATAaaaccgcaaaccg                                                                                                                    <  1:149144/150‑1 (MQ=255)                                               ttGCAAACCCCCACCCCCATCCACACCAACAGCCCCATTACGATCGGTGTTTTCCGCCCAAGGTACACCGACCCCGGGGGGCCCACC‑AGCCAG‑TGGGAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacgga                                                                                                          <  1:806729/149‑1 (MQ=255)                                                      acacCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACaa                                                                                                  >  2:392021/1‑150 (MQ=255)                                                           atcaccatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCg                                                                                            >  2:816521/1‑151 (MQ=255)                                                            acaccatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGc                                                                                           >  1:686571/2‑151 (MQ=255)                                                                 atcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTc                                                                                       >  2:75812/1‑150 (MQ=255)                                                                     aCAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAAt                                                                                  >  1:169194/1‑151 (MQ=255)                                                                          aaCATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGAc                                                                              >  1:810311/1‑150 (MQ=255)                                                                                cccATTCCGTTGGTTGTTTTCCGCGAAATGTCCAGCGCCCCCGGGCGGCGAACC‑ACCCAG‑CGGAAAATACCCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCa                                                                                      <  1:192072/136‑1 (MQ=255)                                                                                    ttACGATCGGTGATTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGc                                                                   >  1:28432/1‑151 (MQ=255)                                                                                      acagtCGGTTTTTTCGGCGCAATTTCCAGCGACCCCGGGCGGCGCATA‑GCCCAG‑TCGAAAATACTCCCCCCCGCCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCt                                                                 <  1:606780/147‑1 (MQ=255)                                                                                        gATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGc                                                               >  1:32392/1‑151 (MQ=255)                                                                                           cGGTGTTTTGCGCGTAATGCTCAGCGCTGCCGCGCGTCGCATC‑TGTCAG‑CATGTGATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCAACAAAACCAGCTTTGAGAATCGACATACCGCGTGCCTGc                                                               >  1:323318/1‑148 (MQ=255)                                                                                            gtttttttACGCGAATTTTCCAGCGACCCCGTGCGCCGCACC‑A‑CCAGTTCGAAAAAAACCCCCCCCCCCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAc                                                             <  1:247675/145‑1 (MQ=255)                                                                                                  ttccgcgCAATTTTCAGCGACCCCGTGCGGCGCACC‑ACCCAG‑TCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATaa                                                      <  2:757498/147‑1 (MQ=255)                                                                                                  ttccgcgAAATGTCCAGCGACCCGGGGGGGCCCTTC‑GCCCAG‑GGGAAAAAAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATaa                                                      <  1:568510/147‑1 (MQ=255)                                                                                                      gcgaattGTTCGGCGACCCGGGGCGGGCAATA‑AGCCGG‑CCGAAAATAATCCCCCCCACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAAcc                                                   <  2:142905/143‑1 (MQ=255)                                                                                                          aatttccAGCGATCCCGTGCGGCG‑ATCAAGCCGG‑TCGAAAATAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAc                                             <  2:1412/145‑1 (MQ=255)                                                                                                              ttCACCGACCCGGGGCGGCGCATC‑CCCCAG‑CCGAAAAAACCCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAg                                          <  1:661002/150‑1 (MQ=255)                                                                                                              tccAGCGCCCCCGGGCGCCGCACC‑ACCCAG‑CCGAAAATACCCCCCCCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAg                                          <  1:399329/148‑1 (MQ=255)                                                                                                                      tCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaataa                                   >  1:638620/1‑149 (MQ=255)                                                                                                                         cGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAcc                                                            >  2:136251/1‑121 (MQ=255)                                                                                                                              ggccCATC‑ACCCAG‑TCGAAAATAACCCCCCCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagtaa                           <  2:678142/149‑1 (MQ=255)                                                                                                                                cccATC‑AGCCAG‑TCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCa                       <  2:226034/149‑1 (MQ=255)                                                                                                                                    tC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGc                   >  2:532098/1‑151 (MQ=255)                                                                                                                                              tCGAAAATACCCCCCCCCACCCCCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGAt             <  2:169218/149‑1 (MQ=255)                                                                                                                                                  aaaTAACCCCCCCCACCAACCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATa            <  1:135913/146‑1 (MQ=255)                                                                                                                                                    aaaatCCCCCCCACCACGCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACt     <  2:353607/149‑1 (MQ=255)                                                                                                                                                      aatCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTg    >  2:222666/1‑150 (MQ=255)                                                                                                                                                       atCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGAc  >  1:212195/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                      GGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATC‑AGCCAG‑TCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGAC  >  NC_000913/2921024‑2921322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑