Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,161,1420CA43.6% 73.8 / 32.6 55Q7K (CAA→AAA) fabRtranscriptional repressor of fabA and fabB
Reads supporting (aligned to +/- strand):  ref base C (23/8);  new base A (0/24);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 4.74e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGT  >  NC_000913/4160992‑4161287
                                                                                                                                                      |                                                                                                                                                 
aaGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGcaac                                                                                                                                                   >  2:311455/1‑151 (MQ=255)
  gAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGcaaca                                                                                                                                                  >  1:120384/1‑150 (MQ=255)
   aaCCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGcaacaa                                                                                                                                                 >  1:389252/1‑150 (MQ=255)
   aaCCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACaaa                                                                                                                                                <  1:380384/151‑1 (MQ=255)
    aCCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACaaa                                                                                                                                                >  2:173648/1‑150 (MQ=255)
     ccGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACaaaa                                                                                                                                               >  2:592877/1‑150 (MQ=255)
       ggCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGa                                                                                                                                            <  2:337559/151‑1 (MQ=255)
        gCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGa                                                                                                                                            >  2:187016/1‑150 (MQ=255)
             aGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGTGTAAGAGCGCAACAAAAAGAAAAAAc                                                                                                                                      >  2:717068/1‑151 (MQ=255)
                aTTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACccg                                                                                                                                   <  2:100609/151‑1 (MQ=255)
                  tGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACccgc                                                                                                                                  <  2:708896/150‑1 (MQ=255)
                     aGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGtt                                                                                                                              >  2:255698/1‑151 (MQ=255)
                          atatGTTTTATTGCTTTCCCGTTCATTCACAATACGGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGCCATCGTGATGGGCGAAAGAGCCCAAAAAAAAGAAAAAACCCGCCGTTCGCt                                                                                                                          <  1:741257/150‑1 (MQ=255)
                                  tATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGc                                                                                                                 >  2:801419/1‑151 (MQ=255)
                                  tATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGc                                                                                                                 >  1:816823/1‑151 (MQ=255)
                                    ttGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCg                                                                                                               >  1:749151/1‑151 (MQ=255)
                                    tggCGTTGCCGTTCATCCACATTACTGGAGCAAGCCAGTTTGTTCTTTCTCTGTTTTAGAGCCAACAGTGTTTTGGGCAAGAATTCAGACCTCGGGGTGGGAGAAAAAGCGCAACAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGcc                                                                                                                <  1:527496/148‑1 (MQ=255)
                                                tcattcaCAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAAt                                                                                                   >  2:529093/1‑151 (MQ=255)
                                                      acaATACTGGAGCAATCCATTATGTTCATTCTCTGGTATAGTGCCAGCATTACTTTTGGCAAGGATTCAGACATCGTGAGGGGCGTAAGAGCGAAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGt                                                                                              <  1:709248/150‑1 (MQ=255)
                                                      aaaaTACTGGAGCAACCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTCTTTTGGGAAAGGATTCAGACATGGTGTTGGGCGAAAGAGCGCAAAAAAAAGAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGt                                                                                              <  1:842720/148‑1 (MQ=255)
                                                        aatgcgggAGCTATCCAGTATTTTCTTTCTCTGGGAAAGTGCCAGCAGTACTTTTGGAAAGGATTCAGCCCTGGGGAGGGGGGAAAGAGCACAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCt                                                                                           <  1:536714/145‑1 (MQ=255)
                                                               gAGCAATCCAGTATTTTCATTTTCTGGTATAGTCCCAGCAGTTCTTTTGGAAAGGATTCAGACATGGTGAGGGGCGTAAGAGCGAAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCa                                                                                    <  1:471097/151‑1 (MQ=255)
                                                                   aaTCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCtt                                                                                >  1:110741/1‑151 (MQ=255)
                                                                   aaTCCAGTATGTTCATTCTCGGGTATAGTGCCAGCAGTACTTTTGGAAAGGATCCAGACATCGTGAGGGGCGAAAGAGCGCAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCtt                                                                                <  1:523337/151‑1 (MQ=255)
                                                                    aTCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTc                                                                               >  2:121294/1‑151 (MQ=255)
                                                                       cggTATTTTCATTCTCTGGTAGAGTCCCAGCAGTGCTTTGGGCAAGGATTCAGACATCGTGATGGGGGTAAGAGCGCAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCg                                                                              <  1:733775/147‑1 (MQ=255)
                                                                       cAGTATTTTCTTTCTGTGGTATAGTGCCAGAAGTACTTTTGGCAAGGTTTCAGACATCGTGATGGGCGAAAGAGCGAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGcc                                                                            <  1:105400/151‑1 (MQ=255)
                                                                        aGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGcc                                                                            >  2:611894/1‑150 (MQ=255)
                                                                          tATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGc                                                                         >  1:148924/1‑151 (MQ=255)
                                                                            tGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCt                                                                       >  1:532318/1‑151 (MQ=255)
                                                                                cATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGAGCTGAACGCAGCTTCGCCAGCCTGAGt                                                                   >  1:878031/1‑151 (MQ=255)
                                                                                  ttCTCTGGTTTAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGtt                                                                  <  2:729320/150‑1 (MQ=255)
                                                                                   tctcTGGTATAGTGCCAGCAGTACTTTGGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGttt                                                                 <  1:440422/150‑1 (MQ=255)
                                                                                      ctGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGt                                                             >  2:482375/1‑151 (MQ=255)
                                                                                            taGTGCCAGCAGTATTTTTGGCAAGGATTCAGACTTCGTGATGGGCGAAAGAGCGAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGt                                                        <  1:741296/150‑1 (MQ=255)
                                                                                             aGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTgg                                                      >  1:350079/1‑151 (MQ=255)
                                                                                              gtcccggcgttACTTTTGACAAGGATGCAGACACGGGGAGGGGCGAAAGAGCGCAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGc                                                               <  1:248415/131‑1 (MQ=255)
                                                                                                 ccAGCAGTACTTTGGCAAAGGATTCAGACACGGGGAGGGGTGTAAGAGCCAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTg                                                       <  1:716977/146‑1 (MQ=255)
                                                                                                 caagcatTATTTTTGGCAAGGATTCAGACATCGGGATGGGCGTAAGAGCGCAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGgcgc                                                   <  2:150494/148‑1 (MQ=255)
                                                                                                  cagcatTATTTTGGGCAGGGATTCAGACATCGTGATGGGCGTAAGAGCGCAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGgcg                                                    <  1:173396/148‑1 (MQ=255)
                                                                                                  cagcagTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGt                                                 >  1:721097/1‑151 (MQ=255)
                                                                                                        tACTTTGGGCAAGGATTCAGACATCGTGAGGGGCGTAAGAGCGAAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGc                                            <  2:389299/150‑1 (MQ=255)
                                                                                                           ttttGGCAAGGATTCAGACATCGTGAGGGGCGTAAGAGCGCAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCgg                                          <  2:816931/149‑1 (MQ=255)
                                                                                                               ggCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTg                                    <  2:782305/151‑1 (MQ=255)
                                                                                                                  aaGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTc                                 >  2:497815/1‑151 (MQ=255)
                                                                                                                     gATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTccc                               >  1:593249/1‑150 (MQ=255)
                                                                                                                      aTTCAGACACCGTAATGGCCGAAAAAGAACAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTccc                               <  2:878150/149‑1 (MQ=255)
                                                                                                                       ttCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCAcc                            >  1:371140/1‑151 (MQ=255)
                                                                                                                            aCATCGTGATGGGGGAAAGAGCACAAAAAAAAGAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCtt                        <  1:53433/150‑1 (MQ=255)
                                                                                                                             ccgcGTGATGGGCGTAAGACCGCAACAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACctct                         <  1:186985/145‑1 (MQ=255)
                                                                                                                             cATCGTGAGGGGCGAAAGAGCGAAACAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACctc                          <  2:515169/147‑1 (MQ=255)
                                                                                                                              aTCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGTACGCTGCTTCGCCAGCCTGAGTTTGCGTGATGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCttttt                     >  1:60103/1‑151 (MQ=255)
                                                                                                                              aTCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCttttt                     >  1:846324/1‑151 (MQ=255)
                                                                                                                                 gtggggggCGAAAGGGCAAAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCttttt                     <  1:255655/143‑1 (MQ=255)
                                                                                                                                   gATGGGCGAAAGAGCGCAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTa                    <  2:155647/147‑1 (MQ=255)
                                                                                                                                      gggTGTAAGAGCGCCAAAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGGAGCGGGCATTGCTCCCACCTCTTTTTATCGGCAt             <  2:216349/151‑1 (MQ=255)
                                                                                                                                            aaGAGCGAAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTcc         <  1:777128/149‑1 (MQ=255)
                                                                                                                                                 cgcAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACg   <  1:795021/150‑1 (MQ=255)
                                                                                                                                                    aacaaAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGt  >  1:336839/1‑148 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
AAGAACCGGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGT  >  NC_000913/4160992‑4161287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: