Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913589,9300AG46.2% 47.0 / 15.1 39L342P (CTC→CCC) nfrAbacteriophage N4 receptor, outer membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (1/20);  new base G (18/0);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 3.05e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTA  >  NC_000913/589784‑590073
                                                                                                                                                  |                                                                                                                                               
gCATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAg                                                                                                                                                <  2:754549/148‑1 (MQ=255)
 cATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGGGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCCCCTCAAGCATTTCATTGGCgggggggggg                                                                                                                                            >  1:33418/1‑149 (MQ=255)
  aTCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGgggggggggg                                                                                                                                           >  2:485185/1‑151 (MQ=255)
         gCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCa                                                                                                                                     <  1:414387/150‑1 (MQ=255)
         gCCAGGTTAGTTGATCCAGGCGGGAAAGGTTTGCCGGGTCTTGCTGATACAACAAAAGGGCCCGACGAAGAGCTTTAACCTTTTTGCGGGTCGCCACCCTGAAAGCATAACGCCCCCCCAGCATTTCATTTGCGgggggggggggcgggc                                                                                                                                     >  1:64052/1‑144 (MQ=255)
               ttAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCCGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTTATTGGCGGGGGGGGGGGGGGGGAGgttttt                                                                                                                              >  2:670787/1‑150 (MQ=255)
                 aGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTgc                                                                                                                             <  2:153820/150‑1 (MQ=255)
                              gggTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTg                                                                                                               <  1:520011/151‑1 (MQ=255)
                               ggTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGa                                                                                                              <  2:46087/151‑1 (MQ=255)
                                     aTTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGAGAGGGTTTTTTGCTGCGTGCTACtttacctt                                                                                                         >  1:679899/1‑143 (MQ=255)
                                     aTTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACcttct                                                                                                        <  1:243001/151‑1 (MQ=255)
                                     aTTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACcttc                                                                                                         <  1:4993/150‑1 (MQ=255)
                                      tttGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGTTTTTTTGCTGCGTCGTACTGACcttctt                                                                                                       >  2:467556/1‑151 (MQ=255)
                                      tttGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGAGCAGGTTTTTCGCTGCGTCGTACTGACcttctt                                                                                                       >  2:45513/1‑151 (MQ=255)
                                           cGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGCTTTTCGCCGGGTCGTACTGGCCTTCctttta                                                                                                   >  1:538540/1‑148 (MQ=255)
                                            ggTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTGCGTCGTACTGACCTTCtttt                                                                                                     >  2:650880/1‑147 (MQ=255)
                                            ggTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACa                                                                                                 <  1:77987/151‑1 (MQ=255)
                                             gTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAg                                                                                                <  1:384355/151‑1 (MQ=255)
                                             gTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGGGGCGAGCAGTTTTTTCGCTGCGTCGTACTGAACTTCtttt                                                                                                     >  2:342870/1‑146 (MQ=255)
                                               tCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTGCGCTGCGTCGTACTGACCTTCTTTTTACAGc                                                                                               >  1:796419/1‑150 (MQ=255)
                                               tCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGGGCAGTTTTTTCGCTGCGTCGTACTGACCTTCTTTTTACAGc                                                                                               >  2:565252/1‑150 (MQ=255)
                                                 ttGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGGGCAGGTTTTTCGCTGCGTCGTACTGACCTTCTTTTTAAAGCAcc                                                                                            >  2:243754/1‑151 (MQ=255)
                                                 ttGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGATGAGTTTTTTTGCTGCCGCGCAGCGATCTTCTTTTAacaacagc                                                                                            >  2:169686/1‑149 (MQ=255)
                                                     tGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGCGCAGGTTTTTGGCGGCGTCGTACTCACCTTTTTTTTAAAGCAc                                                                                             >  1:275837/1‑146 (MQ=255)
                                                      gATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGt                                                                                         <  1:599311/149‑1 (MQ=255)
                                                       aTACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGc                                                                                      <  1:858207/151‑1 (MQ=255)
                                                       aTACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGc                                                                                      <  2:182769/151‑1 (MQ=255)
                                                         aCAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGCAGGTTTTTCGCTGCGGCGTACTGACCCTCTTTTTACAGCACCGGGAGCg                                                                                     >  1:808186/1‑150 (MQ=255)
                                                            gCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTGCGCCGCGGCGTTCTGACCCTCTTTTTAAAACACCgggggcggcg                                                                                  >  1:25624/1‑142 (MQ=255)
                                                               aTCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGGGGTTTTTTGCCGCGTCGGACCGACCTTCTTTTTACAGCACCCGGAGGGGCGCGcc                                                                              >  1:833097/1‑151 (MQ=255)
                                                               aTCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTTGCTGCGGCGTACTGAACTTCTTTTTACAACAACGGGAGGGGCGCGcc                                                                              >  2:646833/1‑151 (MQ=255)
                                                                        gACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATa                                                                     <  2:230424/151‑1 (MQ=255)
                                                                          cGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGAGCAGGTTTTGTGCCGCCCCGTACTGTCCTTCTTTTTACAGCAACGGGAGCGTCGCGCCCACAACATAc                                                                    >  1:494000/1‑150 (MQ=255)
                                                                                   tCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGtt                                                            <  1:670700/149‑1 (MQ=255)
                                                                                        cTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTGCGTCGTACTGACCTTCTTTTTACAACACCGGGAGCGTCGCGCCCACAACAAACTGGCgggggtttg                                                       >  2:625544/1‑142 (MQ=255)
                                                                                               cGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTa                                              <  2:251841/151‑1 (MQ=255)
                                                                                                   tCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGGGGAGGTTTTTCGCTGCGTCGTACTGACCCTCTTTTTACAACACCGGTAGCGTCGCGCCAACAAAATACTGGGGGgtgtttggcca                                                   >  2:731773/1‑135 (MQ=255)
                                                                                                      ccACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGtat                                        <  2:64062/150‑1 (MQ=255)
                                                                                                             gACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAGCTGTACCGTATAATTCGcc                                <  2:237668/151‑1 (MQ=255)
                                                                                                                           cctcAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACg                    <  1:513330/149‑1 (MQ=255)
                                                                                                                                     ttCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCt         <  1:485125/150‑1 (MQ=255)
                                                                                                                                           ggCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTa  <  2:708732/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GCATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTA  >  NC_000913/589784‑590073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑