Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913589,9340TG50.0% 37.6 / 22.2 38T341P (ACC→CCC) nfrAbacteriophage N4 receptor, outer membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/19);  new base G (19/0);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 5.66e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTA  >  NC_000913/589786‑590073
                                                                                                                                                    |                                                                                                                                           
aTCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGgggggggggg                                                                                                                                           >  2:485185/1‑151 (MQ=255)
       gCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCa                                                                                                                                     <  1:414387/150‑1 (MQ=255)
       gCCAGGTTAGTTGATCCAGGCGGGAAAGGTTTGCCGGGTCTTGCTGATACAACAAAAGGGCCCGACGAAGAGCTTTAACCTTTTTGCGGGTCGCCACCCTGAAAGCATAACGCCCCCCCAGCATTTCATTTGCGgggggggggggcgggc                                                                                                                                     >  1:64052/1‑144 (MQ=255)
             ttAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCCGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTTATTGGCGGGGGGGGGGGGGGGGAGgttttt                                                                                                                              >  2:670787/1‑150 (MQ=255)
               aGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTgc                                                                                                                             <  2:153820/150‑1 (MQ=255)
                            gggTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTg                                                                                                               <  1:520011/151‑1 (MQ=255)
                             ggTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGa                                                                                                              <  2:46087/151‑1 (MQ=255)
                                   aTTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGAGAGGGTTTTTTGCTGCGTGCTACtttacctt                                                                                                         >  1:679899/1‑143 (MQ=255)
                                   aTTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACcttct                                                                                                        <  1:243001/151‑1 (MQ=255)
                                   aTTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACcttc                                                                                                         <  1:4993/150‑1 (MQ=255)
                                    tttGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGTTTTTTTGCTGCGTCGTACTGACcttctt                                                                                                       >  2:467556/1‑151 (MQ=255)
                                    tttGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGAGCAGGTTTTTCGCTGCGTCGTACTGACcttctt                                                                                                       >  2:45513/1‑151 (MQ=255)
                                         cGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGCTTTTCGCCGGGTCGTACTGGCCTTCctttta                                                                                                   >  1:538540/1‑148 (MQ=255)
                                          ggTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTGCGTCGTACTGACCTTCtttt                                                                                                     >  2:650880/1‑147 (MQ=255)
                                          ggTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACa                                                                                                 <  1:77987/151‑1 (MQ=255)
                                           gTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAg                                                                                                <  1:384355/151‑1 (MQ=255)
                                           gTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGGGGCGAGCAGTTTTTTCGCTGCGTCGTACTGAACTTCtttt                                                                                                     >  2:342870/1‑146 (MQ=255)
                                             tCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTGCGCTGCGTCGTACTGACCTTCTTTTTACAGc                                                                                               >  1:796419/1‑150 (MQ=255)
                                             tCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGGGCAGTTTTTTCGCTGCGTCGTACTGACCTTCTTTTTACAGc                                                                                               >  2:565252/1‑150 (MQ=255)
                                               ttGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGGGCAGGTTTTTCGCTGCGTCGTACTGACCTTCTTTTTAAAGCAcc                                                                                            >  2:243754/1‑151 (MQ=255)
                                               ttGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGATGAGTTTTTTTGCTGCCGCGCAGCGATCTTCTTTTAacaacagc                                                                                            >  2:169686/1‑149 (MQ=255)
                                                   tGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGCGCAGGTTTTTGGCGGCGTCGTACTCACCTTTTTTTTAAAGCAc                                                                                             >  1:275837/1‑146 (MQ=255)
                                                    gATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGt                                                                                         <  1:599311/149‑1 (MQ=255)
                                                     aTACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGc                                                                                      <  1:858207/151‑1 (MQ=255)
                                                     aTACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGc                                                                                      <  2:182769/151‑1 (MQ=255)
                                                       aCAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGCAGGTTTTTCGCTGCGGCGTACTGACCCTCTTTTTACAGCACCGGGAGCg                                                                                     >  1:808186/1‑150 (MQ=255)
                                                          gCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTGCGCCGCGGCGTTCTGACCCTCTTTTTAAAACACCgggggcggcg                                                                                  >  1:25624/1‑142 (MQ=255)
                                                             aTCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGGGGTTTTTTGCCGCGTCGGACCGACCTTCTTTTTACAGCACCCGGAGGGGCGCGcc                                                                              >  1:833097/1‑151 (MQ=255)
                                                             aTCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTTGCTGCGGCGTACTGAACTTCTTTTTACAACAACGGGAGGGGCGCGcc                                                                              >  2:646833/1‑151 (MQ=255)
                                                                      gACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATa                                                                     <  2:230424/151‑1 (MQ=255)
                                                                        cGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGAGCAGGTTTTGTGCCGCCCCGTACTGTCCTTCTTTTTACAGCAACGGGAGCGTCGCGCCCACAACATAc                                                                    >  1:494000/1‑150 (MQ=255)
                                                                                 tCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGtt                                                            <  1:670700/149‑1 (MQ=255)
                                                                                      cTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTGCGTCGTACTGACCTTCTTTTTACAACACCGGGAGCGTCGCGCCCACAACAAACTGGCgggggtttg                                                       >  2:625544/1‑142 (MQ=255)
                                                                                             cGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTa                                              <  2:251841/151‑1 (MQ=255)
                                                                                                 tCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGGGGAGGTTTTTCGCTGCGTCGTACTGACCCTCTTTTTACAACACCGGTAGCGTCGCGCCAACAAAATACTGGGGGgtgtttggcca                                                   >  2:731773/1‑135 (MQ=255)
                                                                                                    ccACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGtat                                        <  2:64062/150‑1 (MQ=255)
                                                                                                           gACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAGCTGTACCGTATAATTCGcc                                <  2:237668/151‑1 (MQ=255)
                                                                                                                         cctcAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACg                    <  1:513330/149‑1 (MQ=255)
                                                                                                                                   ttCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCt         <  1:485125/150‑1 (MQ=255)
                                                                                                                                         ggCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTa  <  2:708732/151‑1 (MQ=255)
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ATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTA  >  NC_000913/589786‑590073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑