Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	827,507	0	T	G	51.6%	83.6 / 103.0	63	D492A (GAC→GCC)	ybhF	putative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (4/26);  new base G (32/0);  total (37/26)
Fisher's exact test for biased strand distribution p-value = 1.31e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-11

GGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCAT  >  NC_000913/827365‑827652                                                                                                                                               |                                                                                                                                                  ggCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGcc                                                                                                                                            <  1:205056/150‑1 (MQ=255) ggCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGc                                                                                                                                             <  1:339238/149‑1 (MQ=255) ggCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCg                                                                                                                                           <  2:576942/151‑1 (MQ=255) gacGATTAATTTCCCGCGGTACCCCAGGCCGATGCGGTCGCTATATTCCGCTTCACCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGc                                                                                                                                             <  2:735507/147‑1 (MQ=255)  gCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCAACGcccg                                                                                                                                          >  1:893923/1‑149 (MQ=255)    gATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGACgg                                                                                                                                          >  1:887692/1‑149 (MQ=255)           ttCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGt                                                                                                                                  <  1:616003/150‑1 (MQ=255)             cccGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCgg                                                                                                                                   >  2:584870/1‑147 (MQ=255)                    tACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGGAACGCCGGGAGTGGGGTTGTCgag                                                                                                                          >  1:683101/1‑149 (MQ=255)                       acCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACa                                                                                                                     <  2:715630/151‑1 (MQ=255)                         cAGGCTGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGaaa                                                                                                                       >  2:789279/1‑147 (MQ=255)                          aGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCCAAGCCGGAAGGCCGTtccgcga                                                                                                                           >  1:96447/1‑137 (MQ=255)                               gATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTct                                                                                                             <  2:145562/151‑2 (MQ=255)                                  gCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGGAGGCGGGTCGTCGAGAAACAGAATGTCCgg                                                                                                           >  2:388530/1‑150 (MQ=255)                                      tCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGGAGGCGGTTCGTCGAGAAAAAGAATGTCCGGGTCAt                                                                                                      >  1:536462/1‑151 (MQ=255)                                       cGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCAt                                                                                                      <  2:990464/150‑1 (MQ=255)                                         cAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCCAACCCGGGAGTCGGTTCGTCGAGAAACATAATATCCGGTTcc                                                                                                       >  1:493378/1‑146 (MQ=255)                                         cAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGtt                                                                                                         <  1:172070/145‑1 (MQ=255)                                         cAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAGTCGGGTCGTCGAGAAACAGAATGTCCGGtt                                                                                                         >  2:172085/1‑145 (MQ=255)                                          aaTATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCAt                                                                                                  <  1:789219/151‑1 (MQ=255)                                           atatTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAAAAGAATGTCCGGGTCATGCa                                                                                                   >  2:422041/1‑149 (MQ=255)                                             atTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCAACGCCGGAAGTCGGTTTGTCGAGAAAAAGAATGTCCGGGTCATg                                                                                                     >  2:506778/1‑145 (MQ=255)                                             atTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCa                                                                                                <  2:103187/150‑1 (MQ=255)                                                ccGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCCGAAGGCGGGTCGTCGAGAAAAAGAATGTCCgggtta                                                                                                       >  1:205446/1‑136 (MQ=255)                                                 cGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCCACGCCGGAGGCCCGTTCGTCCAAGAACACAATGTCCGGTTCATGcg                                                                                                   >  2:765005/1‑142 (MQ=255)                                                  gCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGaa                                                                                           >  2:169496/1‑150 (MQ=255)                                                    ttCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGGAGGCGGGTCGTCGTGAAAAAGAAAGTCCGGGTCATGCATCAGCGaa                                                                                           >  1:301506/1‑148 (MQ=255)                                                     tCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACa                                                                                         <  1:98724/149‑1 (MQ=255)                                                         ccATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgcca                                                                                    <  1:92943/150‑1 (MQ=255)                                                             aaaGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAAGCCGGAAGTCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGGACAGgccagcgcc                                                                               >  1:815636/1‑151 (MQ=255)                                                                             aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAAc                                                               <  2:142118/151‑1 (MQ=255)                                                                                 ccGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGTCAACGACGGGAGTCGGGTCGTCGGGAAACAGAATGTTCGGGTCAAGCATCAGCGAACAGGCCAGCGCCAGCCGCTGGTTAAAAcc                                                              >  2:706781/1‑148 (MQ=255)                                                                                  cGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCAACGCCGGGAAGCGGTTCGTCGAGAAACAAACAGTCCGGGTCTTTCATCAGCGGAAAAGACCGCGCCCGCCGCCGTTTTAAAACtca                                                           >  1:416844/1‑148 (MQ=255)                                                                                  cGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGGCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaaaaac                                                              >  2:228308/1‑145 (MQ=255)                                                                                    tGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGACGACGCCGGAAGGCGGTTCTTCGAGAAACAGAATGTTCGGTTTCTGCCTCAGGGAACAGGCCCGCGCCCACCGCTGTTTAAAAccca                                                            >  1:444994/1‑145 (MQ=255)                                                                                     gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGGAGTCGGGTTGGCGAGAAACAGAATGGCCGGGTCATGCATCAGCGAACAGGCCAGCGCCCGCCGCTGTTTTAAACCTAATgg                                                        >  2:45409/1‑150 (MQ=255)                                                                                       cGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAg                                                     <  1:764949/151‑1 (MQ=255)                                                                                         ccTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGCGGGGGGCAACGCCGGAAAACGGGTCGTCGCGAAACAGAATGTCCCGGTCCTCCATCAGCGAAAAGGGCCGGCCCCGCCCCTGTTTTAAAACTAATTGGAGtt                                                   >  2:61891/1‑151 (MQ=255)                                                                                         ccTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGtt                                                   <  1:115316/151‑1 (MQ=255)                                                                                          cTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTTAAACCTAATTGCAggt                                                   >  2:542859/1‑148 (MQ=255)                                                                                            ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCa                                                 <  1:152517/150‑1 (MQ=255)                                                                                            ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCa                                                 <  2:706506/150‑1 (MQ=255)                                                                                            ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGGCGGGAATGGGGTCGTCCAGAAAAAGAAAGTTCGGGTCATGCATCAGCGAACAAGGCAGCCCCCGCCGCCGGTTAAAACCTCAAGGCAgattct                                                >  1:787008/1‑146 (MQ=255)                                                                                               tctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCCACGCCCGGAGGCGGGTCGTCGAGAAAAAGAATGTCCGGTTCATGCATCAGCGAACAAGCCAGCGCCAGCCGCCGTTTTAAAACTAAATGCAGGTCATCgg                                             >  2:809757/1‑151 (MQ=255)                                                                                                     aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGTTCGTCGAGAAAAAAAAAGTCCGGTTCATGCATCACCGAAAAGGCCAGCCCCAGCCCCGGTTTAAAACCAAATGGCGGGTCTTCGgggg                                          >  1:814214/1‑148 (MQ=255)                                                                                                     aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGCGAGGGGGGGAACGCCCGAAGATGGCTCCGCGGGAAACAGAAAGTGCGGGTCATTGACCTGCGAACAGGCCCGGGCCCACCGCCGTTTAAAAACCAACGGCAATTCATccgggggg                                        >  1:951124/1‑143 (MQ=255)                                                                                                       gCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAAGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGAAAAGGCCAGCGCCAGCCGCCGTTTAAAAACTAATGGCAGTTCATCGGGGGCgggg                                     >  2:565933/1‑151 (MQ=255)                                                                                                        cTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAAACTAATGGCAGTTCATCGGTGGCGTggg                                    >  2:690657/1‑151 (MQ=255)                                                                                                        cTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAAAAGGCCAGCGCCAGCCGCTGTTTAAAAACTAATGGCAGTTCATCGgggggggggg                                    >  2:1014534/1‑151 (MQ=255)                                                                                                          gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGa                                   <  1:969463/150‑1 (MQ=255)                                                                                                          gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAAAGCCCGGAGGGGGGTTGTCGGGGAAAAGAATATCCCGGTCCTGCATCAGCGAAAAGGCCACCGCCAGCCGCCGTTTAAAAACTAATGGGAGTTCCTCGgtggggtggggg                                  >  1:625524/1‑149 (MQ=255)                                                                                                            tGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGAACAAGGCAGCGCCCGCCGCCGTTTTAAAACCAAAGGGAGGTCATCGGTGggggggggggg                                >  1:825610/1‑150 (MQ=255)                                                                                                            tGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGTCAACGCCGGAAGTCGGGTCGTCGAGAAACAGAATGTCCGGGTCATGCGTCAGCGAAAAGGCCAGCGCCAGCCGCCGTTTAAAACCTAAAGGCAGTTCATCgggggggggggggg                                 >  1:783032/1‑150 (MQ=255)                                                                                                                           aTTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGcc                 <  2:625577/151‑1 (MQ=255)                                                                                                                           aTTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGcc                 <  2:895393/151‑1 (MQ=255)                                                                                                                            ttCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGTTCGTCGGGAAAAAGAATGTCCGGTTCATGCATCAGCGAAAAGGCCAGCGCCAGCCGCTGTTTTAAACCTAATGGCAGTTCATCGGTGGCGGGGGGGGGGGTACtttttctgac                 >  2:1019978/1‑144 (MQ=255)                                                                                                                              cACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGa               <  1:61883/150‑1 (MQ=255)                                                                                                                              cACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGa               <  1:638378/150‑1 (MQ=255)                                                                                                                               aCGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAAc             <  1:873819/151‑1 (MQ=255)                                                                                                                                   cggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCt         >  2:269335/1‑151 (MQ=255)                                                                                                                                        gAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTc    >  2:84396/1‑151 (MQ=255)                                                                                                                                         aGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCa   <  1:624876/151‑1 (MQ=255)                                                                                                                                          gggggTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCAt  <  2:56673/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                  GGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCAT  >  NC_000913/827365‑827652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑