Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	345,328	0	C	A	47.6%	81.1 / 40.5	63	intergenic (+337/‑76)	yahL/yahM	uncharacterized protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (26/7);  new base A (0/30);  total (26/37)
Fisher's exact test for biased strand distribution p-value = 1.20e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACAT  >  NC_000913/345179‑345473                                                                                                                                                      |                                                                                                                                                   aaaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCa                                                                                                                                                   <  1:906/151‑1 (MQ=255)    aaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCaaaa                                                                                                                                                <  2:561184/151‑1 (MQ=255)      aTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑c                                                                                                                                             >  2:707875/1‑151 (MQ=255)             cATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGcc                                                                                                                                         <  2:90443/148‑1 (MQ=255)             cATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCAtt                                                                                                                                      >  1:399771/1‑151 (MQ=255)             cATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCAAAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAC‑CCGc                                                                                                                                          <  1:38719/147‑1 (MQ=255)              aTGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTT‑‑TGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGc                                                                                                                                   >  1:593509/1‑151 (MQ=255)                gCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGc                                                                                                                                   >  1:634469/1‑151 (MQ=255)                      gTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCtcatc                                                                                                                              >  2:612155/1‑150 (MQ=255)                         ttGTGATTGTGTTGGTTGACTTTAATATAAAAAAAATCATAATCCCATCATGTTTGGGTGGGTTTTTTTCTGTATCTTCCGCACCGCACCCAAGCCGGAAAAAAAATATGCTGTAGGCCTAATATAAAAAA‑CCGCCATTAGCTCATCAgg                                                                                                                           <  2:565088/150‑1 (MQ=255)                            tgAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGa                                                                                                                          >  1:771666/1‑148 (MQ=255)                              attgttttgtttGACATTCATATAAAAAAAATCATATTCCCATCATGTTTGTGTGGGTTTTTTTCTGTATCTTCCGCATCGCACTCAACCCGGAAAAAAAATATGCTGTAGGGCTCATATAAAAAA‑CCGCCATTAGCTCATCAGGaa                                                                                                                         <  1:436114/145‑1 (MQ=255)                              attgtgtgGGTAGAATTACAGATAAAAAAAATATTATTGCCATTATGTTTGTGGGGGGTCTTTTCTATCTCTAACCCAGCGCCCCAAACCCCGACAGAAAATTAGTTGAAGGGTTTATTTAAAACT‑CCGCCATTAGCTCATCAGGAagag                                                                                                                      <  1:512356/148‑1 (MQ=255)                              aaTGTTTTGTTTGAAATTAATATAAAAAAAATCAAAATCCCATCTTGTTTGTGTGGGTTTTTTTCTGTATCTTACGCACCGCACTCAAGCCTGACAAAAAATATGCTGAAAGGCTCATATAAAAAA‑CCGCCATTAGCTCATCAGGaa                                                                                                                         <  2:948804/147‑1 (MQ=255)                                  tgtTGGTTGACATTCATATAAAAAAAATCATAATCCCATCTTGTTGGTGTGGGTTCTTTTCTGTATCTTCCGCACCGCACTCAAGCCTGAAAAAAAATATGCTGAAAGGCTAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAg                                                                                                                   <  2:312239/149‑1 (MQ=255)                                      ggttgACATTAATATAAAAAAAATCATAATTCAAACATTTTGGTGGGGGGTTTTTTCTGTATCTTACGCACCGCACCCAGGCCGGACAAAAAATATGCTGAAAGGCTAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAgacga                                                                                                               <  2:498325/149‑1 (MQ=255)                                       gttaACATTCATATAAAAAAAATCATAATCCCATCATTTTTGTGGGGGGTTTTTTCTGTATCTTACGCACCGCACTCAAGCCTGAAAAAAAATATGCTGTAAGGCTCATAACAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAgac                                                                                                                 <  2:535350/146‑1 (MQ=255)                                        ttgACATTCAAATAAAAAAAATCATAATCCCATCATGTTTGTGGGGGGTCTTTCCTTTATCTTACCCATCCCAATCAAGCCTGAAAAAAAATTTGTTGAAAGGCCAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACa                                                                                                             <  2:892663/149‑1 (MQ=255)                                         tgACTTTAATATAAAAAAATTCAAAATCCCATAGTTTTTGTGTGGGTTTTTTTTTGTATCTCACCCACCCCCCCCACCCCTACCAAAAAATATGCTGTAGGCCTCATAAAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAAc                                                                                                           <  1:620019/150‑1 (MQ=255)                                          aacattaaaagaaaaaaaaaCAAAATCCCATAATGTTTGTGGGGGTTTTTTTCTATTTCTTACGCATCACACCCAGCCCAAAAAAAAAATATGGTG‑AAGGCCAAAAAAAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCa                                                                                                         <  1:504129/139‑1 (MQ=255)                                           actttCATAAAAAAAAAATCAAAATCCCACCATGTTTGTGGGGGTTTTTTTTTGTATCTTACGCACCGCCCCCAAGCCTAACAAAAAATATGCTGTAAGGCTAAAAAAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCa                                                                                                         <  1:419863/147‑1 (MQ=255)                                               tCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt                                                                                                    >  1:825308/1‑151 (MQ=255)                                                   atGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgttg                                                                                                >  2:493325/1‑151 (MQ=255)                                                     gAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATtgttgt                                                                                               >  1:330921/1‑150 (MQ=255)                                                     aaaaaaaaTCATAATCCCATCATGTTTGTGGGGGTTTTTTTCTGTATCTTCCCCACCCCACCCAAGCCTGAAAAAAAATATGCTGTAAGGCTCATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTa                                                                                              <  2:641181/150‑1 (MQ=255)                                                     aaaaaaaaTAATAATCCCATCTTTTTTGTGGGGGTTTTTTTCTGTATCTTCCCCACCCCCCACAAGCCTAACAAAAAATATGCTGAAAGCCACATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgtt                                                                                                 <  1:686129/147‑1 (MQ=255)                                                     aaaaaaaaTAAAAATCCCTTCATTTTGGTGGGGGTTTTTTTCTTTTTTTTACGCACCCCACCCAAGCTTAAAAAAAAATATGTTGTAGGCCAAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTa                                                                                              <  1:297835/150‑1 (MQ=255)                                                      aaaaaaaTCATAATCCAATCTTTTTTGTGTGGGTTTTTTTTTGTATCTTACGCACCGCCCCCAACCCTGACAAAAAATATGCTGAAAGCCTCAAAAAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgt                                                                                                  <  1:709334/146‑1 (MQ=255)                                                       aaaaaaTAAATATTCAAGCATGTTTTGGTGGGGTCTTTTTTCTATTTACCCCACCGCACTAAAGCCTGAAAAAAAATGTGTTGTAAGGCAAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATg                                                                                            <  1:717132/151‑1 (MQ=255)                                                        aaaaaTCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg                                                                                           >  2:48459/1‑151 (MQ=255)                                                         aaatTCAAAATCCCATCTTTTTTGTGTGGGGTTTTTTCTGTTTCTGCCGCACCCCACTAAAGCCTGAAAGAAAAAATGTTAAAAGGCTAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGt                                                                                          <  2:823386/151‑1 (MQ=255)                                                                   ttCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCg                                                                                >  1:773475/1‑151 (MQ=255)                                                                    tCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCg                                                                                >  1:848854/1‑150 (MQ=255)                                                                     ccATCATTTTTGTGGGGGGTTTTTTCTATATCTTCCGCACCCCACCAAAGCCTAAAAAAAAATATGCTGAAAGGCTAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAg                                                                              <  1:904971/151‑1 (MQ=255)                                                                             tttGTGTGGGTTTTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAAAAAATATGCTGTAAGGCTCATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGa                                                                       <  2:787286/150‑1 (MQ=255)                                                                             tttGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGAt                                                                      >  1:402954/1‑151 (MQ=255)                                                                              ttGTGGGGGGTTTTTTCTGTATCTTCCGCATCGCACCAAAGCCTAAAAAAAAATATGCTGTAAGGCTAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGAt                                                                      <  2:523579/150‑1 (MQ=255)                                                                                 tgtgGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCg                                                                  >  2:818406/1‑151 (MQ=255)                                                                                  gtgGGTTCTTTTCTTTATCTTCCGCACCCCCCCCAACCCAAAAAAAAAATATGCTGTAAGCCTAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCg                                                                  <  1:529550/150‑1 (MQ=255)                                                                                    ggggTTTTTTTTTTTTCTTACCCATCCCCCACAAGCCTAAAAAAAAATATGCTGTAGGTCTAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCgg                                                                 <  1:150730/149‑1 (MQ=255)                                                                                    ggggTTTTTTCTGTATCTTACGCACCGCACTCAAGCCTGACAAAAAATATGCTGAAAGGCTAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGt                                                                <  2:399802/150‑1 (MQ=255)                                                                                        ttttttCTGTTTCTTCCCCACCGCCCCCAGCCCAAACAAAAAATATGCTGAAAGCCTAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCaa                                                            <  1:51617/148‑1 (MQ=255)                                                                                        ttttttCTATTTCTTGCGCGCCGCCCTAATCCCAGAAAAAAAAAATGCTGTAAGCCTTATACAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCaa                                                            <  2:311349/148‑1 (MQ=255)                                                                                        tCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGAAGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGCTGGCGGTCCAAc                                                           >  1:587676/1‑151 (MQ=255)                                                                                            ttCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACtttt                                                       >  1:46682/1‑151 (MQ=255)                                                                                                 tATCTTACGCACCGCCCACAAGCCTGAAAAAAAATATGCTGAAGGGCTAAAATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCa                                                     <  1:170343/148‑1 (MQ=255)                                                                                                   tCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAAct                                                >  1:336434/1‑151 (MQ=255)                                                                                                        cGCATCGCACTCAAGCCGGACAAAAAATATGCTGTAAGGCTAATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAActct                                              <  2:93058/148‑1 (MQ=255)                                                                                                          cATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTg                                          >  2:55055/1‑150 (MQ=255)                                                                                                             cGCACTCAACCCAGAAAAAAAATATGTGGAAAGGCTCAAATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                      <  2:694555/151‑1 (MQ=255)                                                                                                               cACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                      >  1:142847/1‑149 (MQ=255)                                                                                                                aCTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                      >  1:1002437/1‑148 (MQ=255)                                                                                                                  tCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                      >  2:167066/1‑146 (MQ=255)                                                                                                                       ccgaaaaaaaaaTATGCTGAAAGGCCAATATAAAAAC‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATcc                                <  1:762781/139‑1 (MQ=255)                                                                                                                                 aaTATGTTGTAAGGCTCATAAAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATcc                  <  1:795256/151‑1 (MQ=255)                                                                                                                                     tGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGc              >  2:467035/1‑151 (MQ=255)                                                                                                                                      gCTGAAAGACTCATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCAGCa             <  2:815221/151‑1 (MQ=255)                                                                                                                                        tGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCaa            >  2:124905/1‑150 (MQ=255)                                                                                                                                             ggCTAATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGa      <  2:634522/151‑1 (MQ=255)                                                                                                                                              gCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTg        >  2:649580/1‑148 (MQ=255)                                                                                                                                                tCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAAc    >  2:844819/1‑150 (MQ=255)                                                                                                                                                 cATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGATCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACAt  >  1:190162/1‑151 (MQ=255)                                                                                                                                                  atatAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACAt  <  2:773531/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                   AAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACAT  >  NC_000913/345179‑345473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑