| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,873 | 0 | C | T | 44.7% | 36.8 / 18.8 | 38 | S273N (AGC→AAC) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (17/4); new base T (0/17); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.20e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGC > NC_000913/357739‑357996 | ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGca > 2:450120/1‑150 (MQ=255)ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGca > 2:405875/1‑150 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACaaa < 2:711974/151‑1 (MQ=255) tAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTTCC‑AGCTATTTTTCCGCCGCAACAAAAccgccg > 1:309877/1‑151 (MQ=255) gcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTtct > 2:141516/1‑151 (MQ=255) cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTtctc > 1:377765/1‑151 (MQ=255) aTCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTtctct > 2:480804/1‑151 (MQ=255) cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc > 2:92828/1‑151 (MQ=255) cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc > 2:642902/1‑151 (MQ=255) cattcccACCCGCAGCCCTTTTTCGCCGGCTCCGGTGATTCTTTCCCGCC‑ACCAC‑CCGCCCTTTTCCCCACCCCCTTGTCCAAAAT‑TGCTTTCGCGGCGCCTTTCTGCC‑CCTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCag < 2:119585/144‑1 (MQ=255) aTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagtagt > 2:55331/1‑151 (MQ=255) tCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTgg > 2:87425/1‑151 (MQ=255) ccAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGc > 1:487165/1‑151 (MQ=255) ccGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:603249/1‑151 (MQ=255) cccAGCCCTGTGTCAGCGCCTCCCGTGATTCATTCCCCCC‑ACCAA‑CCGCGTTTTTACCCACCCCCATGTGAAAAAA‑TCCCTGCGGGGGGGTTGTCGGCC‑ATTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc < 1:727708/149‑1 (MQ=255) cGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:38519/1‑150 (MQ=255) aGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggg > 1:312360/1‑147 (MQ=255) gccttgtgTCAGCGCCACCCGTGTTTCATTCCCCCA‑AACAC‑CCGCGCTTTTACCCACCGCCTTGTGAAAAAA‑GGCCTTCGGGGCGGTTGTCTCCC‑ATTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCaa < 1:396986/145‑1 (MQ=255) tgtggatggggttACTGTGATTAATTCCCG‑C‑AACAACCCGGGCTTTTATCCACCGCCTTGCAAAAAAA‑CGCCTTCGGGGCGCCTTTCTGCC‑AGTTATTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCaa < 2:840692/133‑1 (MQ=255) tgccggCGGCCACCGTTTTTCATTCCCCCC‑ACCAC‑CCGCGCTTTTACCAACCCCCTTTTAAAAAAA‑TCCCTGCGGGGCGGCTGTCTCCC‑AGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGaa < 1:244935/143‑1 (MQ=255) aGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGgaaaa > 2:524840/1‑145 (MQ=255) ggCTCCCGTTATTCTTTCCCCCC‑ACCAC‑CCGCCCTTTTACCCAACCCCATG‑GCAAAAATTGCTTCCGGGGGGGCTGTCTGCA‑GGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCttt < 1:597632/149‑1 (MQ=255) gcctccggtatttctttCCCCCAAACAC‑CCGCCCTTTTACCCACCGCCATGTGAAAAAA‑CGCCTGCGCGGGGGCTGTCTCCC‑AGCTTTTTTCCCGCGAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGaaa < 1:55322/128‑1 (MQ=255) ccGTGTTTCATTCCCCCC‑ACCAC‑CCGCGCTTTTATCCACCCCCATGTGAAAAAA‑TCCCTCCGCGGGGGCTGTCTGCC‑ATTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 2:378963/149‑1 (MQ=255) ttCTTTCCCCCC‑ACCAC‑CCGCGCTTTTACCCACCCCCTTGGGAAAAAA‑TCCCTCCGGGGCGGTTTTCTCCC‑AGTTTTTTTCCCGCCACAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCtt < 1:541758/137‑1 (MQ=255) tCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAACTTTTTGCCCGTCAtgt > 2:195146/1‑145 (MQ=37) cccccc‑AACAC‑CCGCGCTTTTACCCACCCCCATGGAAAAAAA‑TCCCTTCGCGGCGGCTGTCTGCC‑AGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTtgtgt < 2:862511/146‑1 (MQ=255) aacac‑cCGCGCTTTTTCCCACCCCAATGTGAAAAAA‑TCCCTCCGCGGCGTCTTTCTCCC‑AGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAAt < 2:321177/150‑1 (MQ=255) cccGCACATTTACCCACCCCCTTGAAAAAAAA‑CCCTTCCGGGGCGTCTGTCTGCC‑AGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATc < 1:65109/146‑1 (MQ=255) ccGCGCTTTTCCCCACCCCCATGTGAAAAAA‑TGCCTTCGCGGCGGTTGTCTCCC‑AGCTTTTTTCCCGCCGAACAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCa < 1:641477/143‑1 (MQ=255) ccGCGCTTTTACCCACCGCCTTGTGAAAAAT‑TGCCTTCGCGGCGGCTGTCTGCC‑ACTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 2:155336/148‑1 (MQ=255) cgcgCATTTACCCAACCCCATGTAAAAAAA‑TGCCTCCGCGGCGGCTGTCTGCC‑AGTTATTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctgct < 2:316885/151‑1 (MQ=255) acgctgtatgtaaaaa‑TACCTACGCGGCGGCT‑AGTGCCTATCTATTTTTCCGCCAAAGGAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtgt < 2:745263/137‑1 (MQ=255) tgCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGAAAAAAATTTAAACCCCCCAAGGTGTGTGGGGATCTCCGCTGGGGACAAAGTGGGAAgg > 2:167454/1‑147 (MQ=255) aaaaaa‑TGCCTTCGGGGCGGCTGTCTGCC‑AGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTg < 2:893929/150‑1 (MQ=255) aaaaa‑TGCCCCCGCGGCGGCTTCCTGCC‑AGTTTTTTTCCCGCAGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGt < 1:281193/149‑1 (MQ=255) aaaaa‑TCCTTCCGGGGCGGTTGTCTGCC‑AGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAg < 2:6437/148‑1 (MQ=255) aa‑TCCCTTCGGGGGGGCTTTCTCCC‑AGTTTTTTTCCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggc < 2:626853/151‑1 (MQ=255) | CCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCC‑AGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGC > NC_000913/357739‑357996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |