Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 650,464 | 0 | A | T | 43.6% | 76.6 / 25.8 | 56 | R9R (CGT→CGA) | citE | citrate lyase, citryl‑ACP lyase (beta) subunit |
Reads supporting (aligned to +/- strand): ref base A (1/30); new base T (24/0); total (26/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e-14 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGC > NC_000913/650317‑650610 | cATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACgt < 1:997777/151‑1 (MQ=255) cggcggGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACgttgtt < 2:799766/151‑1 (MQ=255) ggTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGaa < 2:312727/151‑1 (MQ=255) ggTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCgttttttgagc > 1:742720/1‑143 (MQ=255) gtgtCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAg < 2:199903/151‑1 (MQ=255) gtCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGc < 1:1021234/150‑1 (MQ=255) ttttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGaa < 1:515013/150‑1 (MQ=255) ttttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGa < 1:812587/149‑1 (MQ=255) tttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGaaa < 2:857561/150‑1 (MQ=255) tCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGTTTTTCGGTTTTGTAGCGaaaagg > 2:683639/1‑141 (MQ=255) cACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGAGAAGCGGAAATC‑A‑t < 2:882820/151‑1 (MQ=255) aCGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTTGCGCGAAACGGaaa > 2:690270/1‑146 (MQ=255) aTGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCaa < 1:834591/151‑1 (MQ=255) gCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAAt < 2:197563/150‑1 (MQ=255) tACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTCGGAGTTTTTTGAAGGTTCAGCGAAAAGGGAAAC‑A‑TTGGGaa > 2:418369/1‑147 (MQ=255) ggAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTccc < 2:795921/151‑1 (MQ=255) ggAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGGGTTTAGTTTTTGCAGCGGAACGGAGATA‑A‑ATGGGaaaaccccc > 1:585812/1‑144 (MQ=255) ggAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTTTTGCAGCGAAACGGGAAAC‑A‑TTTGGCATCCTccc > 1:2495/1‑151 (MQ=255) aGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCAt < 2:581554/151‑1 (MQ=255) gTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATg < 2:205044/151‑1 (MQ=255) gTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTTCAGCGGAGCGGAAAAC‑A‑TTTGCCATCCTcccc > 2:78701/1‑148 (MQ=255) ttCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGATTTTCGTTGTTGCAGAGCAGAGGAAATA‑A‑ATggtgaacccccc > 2:785965/1‑135 (MQ=255) cGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCaga < 2:269206/151‑1 (MQ=255) gAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCagag < 1:58179/151‑1 (MQ=255) aaCATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTGGTGCGGAACGGGAAAC‑A‑ATGGGCAACCCCCCCCGGCAGAGGCggg > 1:275776/1‑149 (MQ=255) aaCATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGAGTTTTTCGTTGTTGCAGCGAAGCGGAAAAC‑A‑ATGGCAATCCTCCCCTGGCAGAGCCggg > 2:231153/1‑149 (MQ=255) aTCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGCGGTTTGTGGTTTTTGTGAGACACGGGAATC‑A‑TTTTGAATCCCCCCCCGGCGGGGCCGGGATaa > 2:1009008/1‑149 (MQ=255) tCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGCGGCGGAGCGGGAAAC‑A‑TTTGGAAACCCCCCCTGGCAGAGCCgggggac > 1:70644/1‑145 (MQ=255) cAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGTTTTTCGTTGTTGCAGCGAAGCGGGAAAC‑A‑ATTGCAATCCTTCCACGGCGGAGCCGGGAGACCGc > 1:757645/1‑151 (MQ=255) ggCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGTTTTTAGTTGTTGCAGCGAAGCGGGAATC‑A‑TTGGCAATCCTCCCACGGCAGAGCCGGGAGAcc > 1:338775/1‑146 (MQ=255) aGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTg < 1:616399/151‑1 (MQ=255) ccGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGTTTTTCTTTTTTTCGCCGAAACGGGAAAC‑A‑ATGGGCAACCCCCCATGGCAGGGCCGGGGTACCCCCCGCGCGCGcc > 1:21608/1‑151 (MQ=255) gTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCcagca < 1:678556/151‑1 (MQ=255) aGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGGTGTTGCAGCGAAGCGGGAAAC‑A‑TTGGGAATCCTCCCCTGGCAGAGCCGGGGTAACGCTGGCGCGGGCCCGCAgg > 2:248285/1‑151 (MQ=255) aTGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTCGTTTTTTCAGCGAAGCGGGAAAC‑A‑ATTGCAATCCCCCCCCGGCAGGGCCGGGGTAACGCCGGCGCGGGCCCGCAggg > 1:705684/1‑150 (MQ=255) gAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCt < 1:353546/150‑1 (MQ=255) ttGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTCGTTGTTGCAGCGAAGCGGAAAAC‑A‑ATGGCAATCCTCCCCTGGCAGAGCCGGGGGACCGCTGGCGCGTGCCAGCCGGGCTTcccg > 2:114234/1‑146 (MQ=255) tGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTca < 1:708791/150‑2 (MQ=255) tGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTATGTTTTTGCAGGGAAGAGGAAATCAA‑TTGGCAA‑CCTCCCATCGCAGAGGCCGGGTAACGCCGGCGCGGGGCCGCAGGGGTTTCaat > 2:555449/1‑143 (MQ=255) ccATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGTTTTTCGTTGTTGCAGCGAAGCGGGAAAC‑A‑TTGGCAATCCTCCCATGGCCGAGCCGGGGTACCGCCGGCGCGGGCCAGCAGGGCTTCCAGTCGTTCACGt > 2:319229/1‑151 (MQ=255) cgcgGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTTCGGTTTTGCAGCGAAACGGGAAAC‑A‑TTTGCAAACCTCCCCTGGCAGAGCCGGGGGACCGCTGGCGCGGGCCAGCAGGGCTTCCCGTCGTGCACGTaaa > 1:387239/1‑150 (MQ=255) gCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCa < 2:484314/151‑1 (MQ=255) tGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGGTTTTCGTTGTTTCAGCGAAGCGGAAAAC‑A‑ATGGCAATCCCCCCCTGGCAGAGCCCGGGTAACCCCGGCGCGGGCCCGCAGGGCTTCCAGTCGGGCACGGAAAAAGCAGGcc > 2:913422/1‑151 (MQ=255) tGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTGCAGCGAAGAGGGAAAC‑ATTTGGCAA‑CCTCCCCTGGCAGAGGCGGGGTACCGCTGGCGCGGGCCCGCAGGGCCTCCCGTCGTGCCCGTAAAAAGCAGGcc > 2:964116/1‑151 (MQ=255) ggCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGTTTTTCCATGTTGATACGAAGCGGAAAAC‑A‑TTTGCAATTCCCCCCTGGCAGGGCCGGGGTACCCCGCGGGCGGGCCAGCCGGGGCTCCCCTCGTGCCCggacaa > 1:183061/1‑137 (MQ=255) cACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGt < 2:714318/151‑1 (MQ=255) aGGCACAAACAACATGCTGCGGCGGGTGGGGGGTTTTCGCTTTTGGAGGGAAGAGGGAAAC‑C‑TTGGGAATCCCCCCATGTCAGAGCCGGGGAATCGCCGTGGCGTGCCCACACGGCTTCCCCTGGGGCACGAAAAAAGTAGTcccctg > 1:629432/1‑144 (MQ=255) ggCACAAACAACATGCTGCGGCGGGGGGGAGTTTTTAGGTGTTGCAGCGAAGGGGGAAAC‑A‑TTAGCCATCCTCCCCTGGCAGAAACGGGGAACCCCCGGCGCGTGCCAGCCGGGGTTCCCGTCGTGCACGTAAAAAGCAGTcccg > 2:433678/1‑143 (MQ=255) gCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCt < 2:58291/151‑1 (MQ=255) aacaacATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTc < 2:70652/151‑1 (MQ=255) caacaTGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCAt < 2:2495/151‑1 (MQ=255) gctgcGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACAt < 2:210600/151‑1 (MQ=255) cggGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCta < 1:634073/151‑2 (MQ=255) gTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAc < 2:884381/151‑1 (MQ=255) gTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCcgcg < 1:690217/151‑1 (MQ=255) tttACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCgcgc < 1:948570/150‑1 (MQ=255) | CATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑A‑TTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGC > NC_000913/650317‑650610 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |