Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	665,850	G→T	A155D (GCT→GAT)	mrdB ←	cell wall shape‑determining protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	665,850	0	G	T	100.0%	218.0 / NA	55	A155D (GCT→GAT)	mrdB	cell wall shape‑determining protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/28);  total (27/28)

ATGCATCAGGAAGAACCACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGAAAACGAACAAT  >  NC_000913/665702‑665995                                                                                                                                                     |                                                                                                                                                  aTGCATCAGGAAGAACCACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATcc                                                                                                                                                 <  2:661528/151‑1 (MQ=255) aTGCATCAGGAAGAACCACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATcc                                                                                                                                                 <  2:209328/151‑1 (MQ=255)   gCATCAGGAAGAACCACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATccac                                                                                                                                               <  1:282955/151‑1 (MQ=255)    cATCAGGAAGAACCACAGAATCGGAATGACCGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATccacc                                                                                                                                              >  2:741679/1‑151 (MQ=255)     aTCAGGAAGAACCACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATccacc                                                                                                                                              >  2:529260/1‑150 (MQ=255)       cAGGAAGAACCACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACcagc                                                                                                                                           >  1:549775/1‑151 (MQ=255)               aCCACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTggg                                                                                                                                   <  1:966865/151‑1 (MQ=255)                ccACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGc                                                                                                                                  >  2:17481/1‑151 (MQ=255)                 cacaGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCa                                                                                                                                 <  2:936192/151‑1 (MQ=255)                         cGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATc                                                                                                                         <  1:56320/151‑1 (MQ=255)                                   gccgcTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAgcg                                                                                                               >  2:106382/1‑151 (MQ=255)                                         aCCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGc                                                                                                         >  1:766436/1‑151 (MQ=255)                                            aGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGcc                                                                                                        >  1:994262/1‑149 (MQ=255)                                            aGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAg                                                                                                      <  2:389094/151‑1 (MQ=255)                                              cACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAgt                                                                                                     <  1:280422/150‑1 (MQ=255)                                                     gCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCCCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGttctt                                                                                               <  2:871149/149‑1 (MQ=255)                                                      cGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCa                                                                                             >  1:905087/1‑150 (MQ=255)                                                       gACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAAc                                                                                           >  1:341381/1‑151 (MQ=255)                                                        aCGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACg                                                                                          >  1:464000/1‑151 (MQ=255)                                                         cGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGa                                                                                         <  1:982778/151‑1 (MQ=255)                                                           ccAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATg                                                                                       <  2:597438/151‑1 (MQ=255)                                                             aaTCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGc                                                                                     >  2:348200/1‑151 (MQ=255)                                                                  gACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCa                                                                                <  2:464032/151‑1 (MQ=255)                                                                     gCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAAc                                                                             >  2:297830/1‑151 (MQ=255)                                                                      ccAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACg                                                                            >  1:25269/1‑151 (MQ=255)                                                                       cAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGt                                                                           <  1:1021049/151‑1 (MQ=255)                                                                           tAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTcgcg                                                                       >  2:925060/1‑151 (MQ=255)                                                                            aaGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTcgc                                                                        <  2:729506/149‑1 (MQ=255)                                                                            aaGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCgg                                                                      <  1:754764/151‑1 (MQ=255)                                                                                  agagagGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGAt                                                                 >  1:436538/1‑150 (MQ=255)                                                                                     gagGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATaa                                                               >  2:565766/1‑149 (MQ=255)                                                                                       ggAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAgcg                                                           <  2:424609/151‑1 (MQ=255)                                                                                         aaCAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAgcgcg                                                         >  1:420883/1‑151 (MQ=255)                                                                                          aCAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAgcgcgc                                                        <  1:389866/151‑1 (MQ=255)                                                                                            aGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCaa                                                      <  1:741625/151‑1 (MQ=255)                                                                                                 aaaCAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATc                                                 >  2:940585/1‑151 (MQ=255)                                                                                                  aaCAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATc                                                 >  2:784535/1‑150 (MQ=255)                                                                                                     aGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTg                                             >  1:385140/1‑151 (MQ=255)                                                                                                            aaaGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACgg                                       >  1:663411/1‑150 (MQ=255)                                                                                                            aaaGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGc                                      <  2:85830/151‑1 (MQ=255)                                                                                                                 gcAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGGTGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTAttt                                 >  1:30196/1‑151 (MQ=255)                                                                                                                 gcAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTAttt                                 <  2:902532/151‑1 (MQ=255)                                                                                                                  cAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGGTAAAGCGCGCAACCATCAGTGGTACGGCTAtttt                                <  1:264078/151‑1 (MQ=255)                                                                                                                   aaCGAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTg                               >  1:528497/1‑151 (MQ=255)                                                                                                                      gAGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCa                            <  1:665785/151‑1 (MQ=255)                                                                                                                       aGGATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCa                            >  1:87140/1‑150 (MQ=255)                                                                                                                        ggATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAAt                          <  2:287446/151‑1 (MQ=255)                                                                                                                         gATTGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAAtt                         >  2:338366/1‑151 (MQ=255)                                                                                                                            tGATGTTCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTc                       <  1:594558/150‑1 (MQ=255)                                                                                                                              aTGTTCCCAGGTCAGGCTGTGCATCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGTCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCCATTTTGGCAATTTCCg                     >  1:558171/1‑150 (MQ=255)                                                                                                                                  tCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACgg                 <  1:565720/150‑1 (MQ=255)                                                                                                                                  tCCCAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACgg                 <  1:924675/150‑1 (MQ=255)                                                                                                                                     cAGGTCAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTg              >  1:536756/1‑150 (MQ=255)                                                                                                                                          cAGGCTGTGCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGAAAAc         <  2:623372/150‑1 (MQ=255)                                                                                                                                                gtgCATCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGAAAACGAACAAt  <  2:385170/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  ATGCATCAGGAAGAACCACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGAAAACGAACAAT  >  NC_000913/665702‑665995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑