Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA43.1% 73.1 / 33.1 58intergenic (‑119/+84)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (29/4);  new base A (0/25);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 2.72e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824910‑1825205
                                                                                                                                                     |                                                                                                                                                     
aCATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaa                                                                                                                                                    >  2:493245/1‑150 (MQ=255)
 cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaa                                                                                                                                                    >  2:759061/1‑149 (MQ=255)
  aTTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaaga                                                                                                                                                 <  2:360083/151‑1 (MQ=255)
    tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaa                                                                                                                                                <  1:406216/150‑1 (MQ=255)
      tACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAc                                                                                                                                             >  1:1017656/1‑151 (MQ=255)
       aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAcc                                                                                                                                            <  2:609167/151‑1 (MQ=255)
        cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                           >  2:710376/1‑151 (MQ=255)
         gCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCCTTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACACCTGTTTGAGAAGAGAAAAGAAAAcagc                                                                                                                                          >  2:781773/1‑151 (MQ=255)
              tCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATc                                                                                                                                     >  1:817269/1‑151 (MQ=255)
                    tATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                               >  2:568932/1‑151 (MQ=255)
                       tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                            >  1:572448/1‑151 (MQ=255)
                             cTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt                                                                                                                       >  1:791861/1‑150 (MQ=255)
                              tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                     >  2:1030073/1‑151 (MQ=255)
                                gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                   >  2:192469/1‑151 (MQ=255)
                                 cTCCCGCCCTTTTTTTTTCATTTTTATCGTTCAGGTACCGGTGGATGTTTACCGGCAACTGGGGTGATTTACGTTGGTGGTGATCAGTTTTTTTTAACACCTGTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTa                                                                                                                      <  1:714040/147‑1 (MQ=255)
                                  tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                  >  2:860631/1‑150 (MQ=255)
                                   cccgccctttttttttCTTTGTTTTCTTTAGGGTACCCGGGGTGGTTTTTCGCAACCTGGGGTGTTTTCCTTTAGTGTTAATCAGGTTTTTTTTAAACTTTTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                        <  1:1051748/131‑1 (MQ=255)
                                    ccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCa                                                                                                                 >  1:925761/1‑149 (MQ=255)
                                      ggCCCGTTTTTTTCTTTGTTATCGTTCAGGTACCCGGGGATGTTTATCGGCACCTGGGGTGATTTACGTTAGGGGTGACCAGGTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAg                                                                                                               <  1:98985/149‑1 (MQ=255)
                                      ggCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                             >  1:556607/1‑151 (MQ=255)
                                       gCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                             >  2:308654/1‑150 (MQ=255)
                                        cccGTTTTTTTCATTTTTATCGTTAAGGTACCGGTGGATGTTTATCGGCAACTGGGGTGATTTAGTTTGGGGGTGATCAGGTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg                                                                                                           <  1:493244/151‑1 (MQ=255)
                                         ccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGt                                                                                                          >  1:179674/1‑151 (MQ=255)
                                          cGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                         >  1:398017/1‑151 (MQ=255)
                                               ttttCTTTTTTATGGTTCAGGAAACCGGGGATGTTTATCGGCACCTGGGGTGTTTTACGTTGGTGGTGATCGGGTTTTTTTTACAATTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                      <  2:563694/149‑1 (MQ=255)
                                               ttttCTTTGTTATCTTTCAGGAACCGGGGGATGTTTATCGGCAACTGGGTTGTTTTCCGTTGGGGTTGATCGGGTTTTTTTTACACCTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                     <  2:233496/150‑1 (MQ=255)
                                               ttttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                     >  2:755985/1‑150 (MQ=255)
                                                   cATTGTTATCGTTAAGGTACCCGTGGATGGTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGACCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAc                                                                                                 <  1:942069/150‑1 (MQ=255)
                                                        ttgtggtgcAGGTACCCGTGAATGTTTACCGGCACCTGGGTTATTTTAGTTTGGTGGTGACCAGGTTTTTTTTACACCTGTTGGAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                             <  1:568911/141‑1 (MQ=255)
                                                        ttATCTTTCAGTAAACCGTGGTTGTTTATCGGCAACTGGGGTATTTTACGTTAGTGGTGACCGGTTTTTTTTAACAACTGTTAAAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                             <  2:414579/149‑1 (MQ=255)
                                                        ttATCGTTCAGGTACCCGTGGATGGTTTTCGGCAACGGGGGTATTTTATTTTAGTGGTGATCAGTTTTTTTTTACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                             <  2:113184/149‑1 (MQ=255)
                                                                  gctgcctggggATGGTCATGGCAATCGGGGTGGATTTCCATTTGTGGTGACCAGTTTTTTTTAAAATCTTTTTGAAAGAAAAAAAGAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttt                                                                                 <  1:472858/142‑1 (MQ=255)
                                                                       cgggggATGTTTATCGGCACCTGGGGTGATTTACTTTGGTGGTGATCGGTTTTTTTTAACAATTTTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttttt                                                                               <  2:791884/144‑1 (MQ=255)
                                                                              tGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGATTTTTTGATGaa                                                                       >  2:411403/1‑149 (MQ=255)
                                                                                  taccGGCAGCTGGGTTGTTTTACGTTGGTGGTGATCGGTTTTTTTTTACAACTTTTGGAAAAAAAAAAAAAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt                                                                  <  1:978082/147‑1 (MQ=255)
                                                                                         aaCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                           >  2:731336/1‑150 (MQ=255)
                                                                                            ctggggTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTTTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca                                                          <  1:139349/146‑1 (MQ=255)
                                                                                                        tACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                             >  2:506502/1‑151 (MQ=255)
                                                                                                          ctttAGTGGTGATCGGTTTTTTTTACCACTTTTTAGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                             <  2:110968/147‑1 (MQ=255)
                                                                                                          cGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                            >  2:524416/1‑150 (MQ=255)
                                                                                                            ttAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAc                                          <  1:276108/150‑1 (MQ=255)
                                                                                                            taaGTGGTGATCGGGTTTTTTTTACACTTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTCGCAGGCAttt                                             <  2:867090/145‑1 (MQ=255)
                                                                                                               gtggtgATCGGGTTTTTTTTACATCTTTTGGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                      <  1:759043/151‑1 (MQ=255)
                                                                                                                 ggttATCAGGTTTTTTTTACAACTTTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                            <  1:928992/143‑1 (MQ=255)
                                                                                                                 ggtgATCGGTTTTTTTTTACACTTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                      <  2:572468/149‑1 (MQ=255)
                                                                                                                 ggtgATCGGTTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt                                     <  2:1106706/150‑1 (MQ=255)
                                                                                                                   tgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt                                     >  2:174874/1‑148 (MQ=255)
                                                                                                                     aTCAGCTTTTTTTTCCAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGc                                <  1:292634/151‑1 (MQ=255)
                                                                                                                       cAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTg                              >  1:734686/1‑151 (MQ=255)
                                                                                                                          ttttttttcACATTTGTATAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGc                                <  2:1017694/144‑1 (MQ=255)
                                                                                                                           cTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGAAAGATTATTCAGGAGTTGCAGGCGTTTTACCTTTTGCCGccg                              >  1:1058451/1‑145 (MQ=255)
                                                                                                                            tttttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGt                         >  1:249406/1‑151 (MQ=255)
                                                                                                                            tttttttACAACTGTTTGAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACg                          <  1:693380/150‑1 (MQ=255)
                                                                                                                            ttttttaACAACTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGa                            <  2:925796/148‑1 (MQ=255)
                                                                                                                              tttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGTTAGGTAGTGGACAAGACCGGCGGTTTTAAGATTTTTTGACAAAAGAATATTCAGTAGCTGCATGCAATTTAACCTTTTCCGCTCGACGt                         >  2:443630/1‑149 (MQ=255)
                                                                                                                              tttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                        >  2:567589/1‑150 (MQ=255)
                                                                                                                                 ttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                    >  2:57894/1‑151 (MQ=255)
                                                                                                                                   aCAACTGTTTAAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa                  <  1:890718/151‑1 (MQ=255)
                                                                                                                                     accTGTTGGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGa                <  1:308645/149‑1 (MQ=255)
                                                                                                                                     aaCTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                    >  2:758703/1‑147 (MQ=255)
                                                                                                                                          tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt           >  2:151205/1‑151 (MQ=255)
                                                                                                                                          tttGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGc             <  2:817294/149‑1 (MQ=255)
                                                                                                                                           ttgaagaagaaaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc               <  2:738189/143‑1 (MQ=255)
                                                                                                                                               agaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTtctc        >  2:9211/1‑150 (MQ=255)
                                                                                                                                                 aagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa     <  1:710359/151‑1 (MQ=255)
                                                                                                                                                 aagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa     <  1:860599/151‑1 (MQ=255)
                                                                                                                                                 aagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa       <  2:398015/149‑1 (MQ=255)
                                                                                                                                                  agagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCaaa     >  2:34515/1‑150 (MQ=255)
                                                                                                                                                    agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCCGCAGTTGCCGGCCTTTTACCTTTTTCCGCTGGACGGTTTGTCAGACGCTTCTCAAAAtt  >  2:316025/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824910‑1825205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: