Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,336,901 | 0 | T | A | 41.1% | 69.3 / 26.9 | 56 | Y44F (TAT→TTT) | adiC | arginine:agmatine antiporter |
Reads supporting (aligned to +/- strand): ref base T (24/9); new base A (0/23); total (24/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 9.18e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGG > NC_000913/4336756‑4337050 | gagGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATg > 1:689805/1‑151 (MQ=255) gagGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATg < 2:717954/151‑1 (MQ=255) gACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑c > 1:669635/1‑150 (MQ=255) cGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAAt > 1:902783/1‑151 (MQ=255) aTAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATACATAAATGG‑CAATTCCGcc > 2:796884/1‑147 (MQ=255) gggCCAAGGCAGCGGCGGGTATAAGCGTAAAAACCACCGGGCTTTGGG‑TCGAGAAACTACTTTTGGGATTTTCCCACCGAGCCCCCGAGTGCACTGATAATCGTCACCAACCACCCAAAAGGGG‑CAATCCCGCCAGTAGAGGCCAGGTTTg < 2:595435/151‑1 (MQ=255) cAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGACCCAGTGCACCGATAATCGTCACCAAACATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAgg > 2:142852/1‑151 (MQ=255) aaagagggggcgggggAAAGCGAAAGACCCCCCAGGATTGGGG‑GCGAGAAACAACATTTGGGCGTATCCCACCGAGAGCCCCAGTGCACCGAAAATCGTCCCCACCCATCCAAAAAGGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAg < 2:791416/141‑1 (MQ=255) aggcagcGGGGGGCGTAGGCATAAAACCCACCAGG‑CTTGGGTTCGAGGACCGACTTTTTGGCTTATCCCACCGAGACCCCCAGTGCACCGATACTCGTCCCCACCCATCAATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTa < 2:399371/149‑1 (MQ=255) cagcGGGGGGCGTAAGCATAAGAACCCCCAGGACTTGGG‑TCGAGGAACGACATTTGGGCGTATCCCATCGAGACCCCCAGTGCCCCGATAATCGTCACCACCCATCCAAAAAGGA‑AAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTaa < 1:935775/149‑1 (MQ=255) taagcgtaagAACCACCAGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTg > 1:1059663/1‑151 (MQ=255) aagctaaagACCCCCCAGGATTGGGG‑TGGAGGAACGAAATTTTGGCGTATACCAGCGAGACCCCCAGTGCACAGATATTCGCCACAAACCACCAAAAAATGA‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTg < 2:505172/144‑1 (MQ=255) aagcgtaagACCCACCAGGATTGGGG‑TCGAGGAACGACATTTGGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCCCCACCCATCCAAAAATGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTg < 2:365286/150‑1 (MQ=255) ctaaaaacccaccgGGACTTGGG‑GCGAGAAACTACTTTTGGGCGTATACCATAGAGAGCCCCAGTGCCCCTATATCCGTCCCAACCCATCAAAAAATGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGAcc < 2:372037/142‑1 (MQ=255) agACCCACCAGGACTTGGG‑TCGAGGAACGACTTTTTGGCGTATCCCATCGAGACCCCCAGTCCACCGATAATCGTCACCACCCACCAAAAAATGA‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCata < 2:322662/151‑1 (MQ=255) accaccAGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCataat > 2:736688/1‑150 (MQ=255) aGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTcccc > 1:370291/1‑151 (MQ=255) aGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑AAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTcccc > 1:1020604/1‑151 (MQ=255) ggACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCCCCGATAATCGTCACCAACCACCCAAAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCg < 1:541279/151‑1 (MQ=255) ggACTGGGG‑TCGAGGAACGACATTTTGGCGTATCCCATCGAGGCCCCCAGTGCCCCGATAACCGTCACAAACCACCCAAAAATGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCg < 1:712627/151‑1 (MQ=255) ggg‑TCGAGGAACGACATTTGGGCGTATACCAGCGAGACCCCCAGTGCACCGATAATCGCCCCCAACCATCCAAAAATGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCa < 2:999418/151‑1 (MQ=255) tCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATc > 2:441601/1‑150 (MQ=255) ggAACGACATTTTGGCGTATCCCATCGAGACCCCCGGTCCACCGATAACCGCCCCAAACCATCCAAAAATGC‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGt < 2:23953/151‑1 (MQ=255) aaCGACATTTTGGCGTATCCCATCGAGAGCCCCAGTGCACCGATAATCTTCACCAACCACCAAAAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGa < 1:1119249/151‑1 (MQ=255) aCATTTTGGCGTATCCCATCGAGAGCCCCAGTGCACCGATAATCGCCACCACCCACCCAAAAAGGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACggg < 1:976951/151‑1 (MQ=255) tttgGGCGTATCCCATCGAGAGCCCCAGTGCACCGATAATCGTCACCACCCATCCATAAAGGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGa < 1:1035564/150‑1 (MQ=255) tttGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGAtt > 2:729825/1‑151 (MQ=255) tttGGCGAATACCAACGAGCGCCCCAGTGCACCGATAACCGCCACCACCCACCCATAAATGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGAt < 1:502903/150‑1 (MQ=255) ttGGCGTATCCCATCGAGAGCCCCAGTGCACCGATAATCGTCCCCAACCACCCAAAAATGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGAtt < 1:872405/150‑1 (MQ=255) ggCGTATCCCACCGAGACCCCCAGTGCCCCGATAATCGTCACCACCCACCCATAAAGGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTaa < 1:767906/150‑1 (MQ=255) gTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGccc > 1:113369/1‑151 (MQ=255) taacctaTCGAGCGCCCAGGGCCACAGATAGGCGCCGCCACCCATCAAAAAACGC‑CACTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGcc < 2:349236/143‑1 (MQ=255) ataCCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCAc > 1:1063061/1‑151 (MQ=255) ataCCAGGGAGAGCCCCAGGGCACCGATAATCGTCACCACCCACCCATAAAGGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGccc < 2:264302/149‑1 (MQ=255) ccATCGAGACCCCCAGTGCACCGATAATCGTCACCACCCATCCAAAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACt < 1:829173/149‑1 (MQ=255) aTCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTtgt > 1:644988/1‑151 (MQ=255) cGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTtgtg > 1:1115064/1‑150 (MQ=255) gagaGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTtgtg > 2:1039716/1‑149 (MQ=255) agagCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTtgtg < 1:620052/148‑1 (MQ=255) ccccAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatc > 1:451025/1‑150 (MQ=255) ccccAGTGCACAGATAATCGTCACCAACCATCCATAAAGGC‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcat < 1:529113/149‑1 (MQ=255) cAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagc > 2:487185/1‑150 (MQ=255) ccGATAATCGCCACCAACCATCCAAAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAg < 1:51378/151‑1 (MQ=255) gATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGAc > 2:479454/1‑151 (MQ=255) aTAATCGTCCCCAACCATCCATAAAGGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGa < 1:459734/146‑1 (MQ=255) aTAACCGCCACCACCCACCAAAAAAGGC‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGAc < 2:513848/150‑1 (MQ=255) aaccgcccccaaccaCCCAAAAATGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATa < 2:508192/143‑1 (MQ=255) aTCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATa > 1:1056446/1‑150 (MQ=255) cgccACCAA‑CACCCAAAAAAGGCCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAt < 2:217092/147‑1 (MQ=255) cGTCACAACCGATCAAAAAAGGG‑CAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAt < 2:800593/150‑1 (MQ=255) tCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTa > 2:442190/1‑150 (MQ=255) caccacCCACCCAAAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaaa < 1:729164/151‑1 (MQ=255) cACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaa > 1:1087049/1‑150 (MQ=255) accaaccaTCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAAc > 1:868815/1‑151 (MQ=255) ccaaccaTCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACt > 1:153841/1‑151 (MQ=255) aaccaTCAAAAAAGGG‑CATTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAt < 2:689822/143‑1 (MQ=255) ccatccatAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTg > 1:22046/1‑151 (MQ=255) catcaaaAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGc < 1:54326/151‑1 (MQ=255) atAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAgg > 2:13172/1‑151 (MQ=255) | GAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGG‑TCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGG‑CAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGG > NC_000913/4336756‑4337050 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |