Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	47.6%	88.4 / 18.7	63	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (6/27);  new base G (30/0);  total (36/27)
Fisher's exact test for biased strand distribution p-value = 4.12e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437                                                                                                                                                    |                                                                                                                                                       ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTg                                                                                                                                                       <  2:703484/149‑1 (MQ=255)  gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaaa                                                                                                                                                    <  2:100820/151‑1 (MQ=255)     cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGAt                                                                                                                                                 <  2:173945/151‑1 (MQ=255)      aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCAGCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATg                                                                                                                                                <  1:516516/151‑1 (MQ=255)        ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgc                                                                                                                                               <  2:517626/150‑1 (MQ=255)         cGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATgcg                                                                                                                                              >  1:746834/1‑150 (MQ=255)             aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCt                                                                                                                                            >  1:369176/1‑148 (MQ=255)                cTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                         <  1:1036469/148‑1 (MQ=255)                   aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCa                                                                                                                                   <  2:854695/151‑1 (MQ=255)                      gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑ggt                                                                                                                               <  1:564575/151‑1 (MQ=255)                            aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCA‑ggtggcgg                                                                                                                          >  1:700178/1‑150 (MQ=255)                               ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGA‑gggggcggggg                                                                                                                       >  1:896496/1‑150 (MQ=255)                                tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑ggtggcggtggc                                                                                                                      <  1:594424/150‑1 (MQ=255)                                  ttCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGGAAGATGCGCTGAAAGGGCC‑Gggggggggggcg                                                                                                                     >  1:203120/1‑149 (MQ=255)                                   tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGGAAGGGCA‑GGGGGCGGGGGGGttt                                                                                                                  >  1:606813/1‑151 (MQ=255)                                     tcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGA‑GGGGGGGGGGGGGGTTg                                                                                                                 >  1:479826/1‑150 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCA‑GGGGGCGGGGGGGGTTg                                                                                                                 >  1:444022/1‑149 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGCA‑ggtgggggggggggtt                                                                                                                  >  1:1996/1‑143 (MQ=255)                                        ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCAt                                                                                                             <  1:379600/151‑1 (MQ=255)                                          cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGAGGAAGTTGGGGGGGAAGGAGGGCTGTAAGGGCA‑AGGGGGGGGGGCGgttcgcgatt                                                                                                           >  2:812252/1‑144 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGaaa                                                                                                      <  1:213202/150‑1 (MQ=255)                                                   gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGAATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGGGGGGGGAAAGAAGCGGAGTAAGGGCAGGGGGGGGGTGGGGttttggtttcgtga                                                                                                       >  2:808166/1‑136 (MQ=255)                                                    aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCC‑GGGGGGGGGGGGGGTTGTCATTCCGAAagag                                                                                                   >  1:1048583/1‑149 (MQ=255)                                                     tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAATGCGCTGAAAAGGCA‑GGTGGCGGGGGGGGTTGTCATTCCGGAaga                                                                                                    >  1:546716/1‑147 (MQ=255)                                                       tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGAAAGGGGA‑GGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAACg                                                                                               >  2:947672/1‑150 (MQ=255)                                                            aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGC‑GGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCg                                                                                               >  1:910896/1‑145 (MQ=255)                                                                   ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGa                                                                                  <  1:98444/151‑1 (MQ=255)                                                                          tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCTTAAAGGGGA‑GGGGGGGGTGGGGGTTGTCATTCCGAAAGAGAGCGGCAGGCTGGGAGATCGTg                                                                             >  2:456148/1‑149 (MQ=255)                                                                               tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCCGGAAGGGGC‑GGGGGGGGGGGCGGTTTGCATTCCGACAAAGAACGAGCGGCTGGAAGAACGTggt                                                                           >  2:875546/1‑144 (MQ=255)                                                                                     cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCA‑GGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                                >  2:338517/1‑151 (MQ=255)                                                                                     cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAAGGCA‑GGGGGCGGTGGCGTTTTTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCCCTc                                                                >  2:362869/1‑151 (MQ=255)                                                                                         cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGA‑GGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGCGCACTCGc                                                              >  2:473962/1‑149 (MQ=255)                                                                                          gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCA‑GGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGGGCACTCg                                                               >  1:69988/1‑147 (MQ=255)                                                                                           agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGGCAGTCTGGGAGATCGGGGTGTGGCGCACTCGCa                                                             >  1:116530/1‑148 (MQ=255)                                                                                           agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAAATGCGCTGGAAGGGCA‑GGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGGTGTGGGGCAATc                                                                >  2:426692/1‑145 (MQ=255)                                                                                            gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                         <  1:468517/151‑1 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                       <  2:206886/150‑1 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGGGCCGGAAGGGCA‑GGTGGCGGGGGCGGTTGTTCTTCCGGAAGAGGGGGGCCGTCTGGAAGATCGTGGTGTGGCGCACTCGCCag                                                           >  2:697152/1‑146 (MQ=255)                                                                                                gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGGGGCGGTGGCGTTTGTCATTCCGGAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                       >  1:679223/1‑149 (MQ=255)                                                                                                gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAAGGCA‑GGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAgg                                                     >  1:275475/1‑151 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                    <  2:760208/151‑1 (MQ=255)                                                                                                  agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGCTGGGGGGAAAAATGCGCTGAAAGGGGA‑AGTGGCGGGGGGGGTTGTCACTTCGAAAGAGAGCGAAAGACTGGAAGAACGTGATGGGGCGGACTCGCAAGAGAAAGCg                                                   >  1:52260/1‑151 (MQ=255)                                                                                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                                <  2:130865/151‑1 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcg                                          <  1:462855/149‑1 (MQ=255)                                                                                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtgg                                      <  2:338006/151‑1 (MQ=255)                                                                                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGGAAGGGCA‑GGTGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGGTTATGGGGCtgg                                      >  2:133016/1‑151 (MQ=255)                                                                                                                  cATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                                   <  2:171074/151‑1 (MQ=255)                                                                                                                   aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGGAAGATGCGCTGAAAGGGCA‑GGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                                   >  1:488233/1‑150 (MQ=255)                                                                                                                     ccGGGCGTTGCCGAAGTGGCGGTGTTTGGGGGGAAAGATGCTCTGTAAGGGGA‑GGTGGCGGTGGGGGTTGTCATTTCCTTAGAGAGGGGCAATCTGGAAGATCGGGATGTGGCGCCCTCCCAAGAGAAGGCGATTATTGGGCttgggggc                                 >  2:830752/1‑143 (MQ=255)                                                                                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                                <  1:1104520/151‑1 (MQ=255)                                                                                                                     ccGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                                   >  1:929958/1‑148 (MQ=255)                                                                                                                           gTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGa                          <  2:298256/151‑1 (MQ=255)                                                                                                                           gTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGa                          <  1:947638/151‑1 (MQ=255)                                                                                                                             tGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAt                         <  2:546734/150‑1 (MQ=255)                                                                                                                              gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                        <  1:473963/150‑1 (MQ=255)                                                                                                                               ccGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCC‑GGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGGGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTgg                      >  2:282003/1‑151 (MQ=255)                                                                                                                                cGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCTGAAAGGGCA‑GGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGGGAAAGTCTGGAAGAGCGGGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGGGGGCAGCCAGATTgg                      >  2:803046/1‑150 (MQ=255)                                                                                                                                 gAAGTGGCGGGGGGTGGGGGGGAAAATGCGCCGAAAGGGCA‑GGTGGCGGGGGGGGTTTTCCTTTCGAAAGAGAGGGACAGTCTGGAAGAACGGGGAGGGGCGCACTCGCAAGAGAAGGCGATTATGGGGCTGGGGGGCAGCCAGATTgg                      >  2:679505/1‑149 (MQ=255)                                                                                                                                       gCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg              >  1:212688/1‑151 (MQ=255)                                                                                                                                          gtggtTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc           <  1:1076937/151‑1 (MQ=255)                                                                                                                                             gtTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgcc        >  1:521912/1‑151 (MQ=255)                                                                                                                                                ggggTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg      <  2:792904/150‑1 (MQ=255)                                                                                                                                                   gTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  <  2:444020/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                       GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑