Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,300	G→A	P263L (CCG→CTG)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,300	0	G	A	100.0%	293.3 / NA	73	P263L (CCG→CTG)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/45);  total (28/45)

CCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGG  >  NC_000913/3400153‑3400447                                                                                                                                                     |                                                                                                                                                    ccGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGtt                                                                                                                                                  <  2:681003/151‑1 (MQ=255)  cGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTc                                                                                                                                                 <  1:991472/151‑1 (MQ=255)   ggTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTcc                                                                                                                                                <  2:925466/151‑1 (MQ=255)    gTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCt                                                                                                                                               >  1:206412/1‑151 (MQ=255)      ttcttcCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTg                                                                                                                                              <  1:783906/150‑1 (MQ=255)             aTTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCtt                                                                                                                                      <  1:415116/151‑1 (MQ=255)                aaCAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCg                                                                                                                                    >  1:413506/1‑150 (MQ=255)                 aCAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCtt                                                                                                                                      >  1:649825/1‑148 (MQ=255)                    aaCGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGAt                                                                                                                               <  2:747953/151‑1 (MQ=255)                          cAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCAt                                                                                                                          <  1:265757/150‑1 (MQ=255)                                      gcagTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAggg                                                                                                              >  1:181335/1‑150 (MQ=255)                                      gcagTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAggg                                                                                                              <  2:413502/150‑1 (MQ=255)                                      gcagTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGt                                                                                                             <  1:7154/151‑1 (MQ=255)                                       cagTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTa                                                                                                            <  2:41287/151‑1 (MQ=255)                                       cagTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTa                                                                                                            <  1:313599/151‑1 (MQ=255)                                        agTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTa                                                                                                            >  2:943107/1‑150 (MQ=255)                                            cgcCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAAcc                                                                                                       >  2:940234/1‑151 (MQ=255)                                              ccaccaCCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACcgc                                                                                                     <  1:801442/151‑1 (MQ=255)                                               caccacCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACcgcg                                                                                                    <  1:798393/151‑1 (MQ=255)                                               caccacCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACcgcg                                                                                                    <  1:188623/151‑1 (MQ=255)                                                accaccGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGt                                                                                                   <  1:1081484/151‑1 (MQ=255)                                                 ccaccGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGt                                                                                                   <  2:1118233/150‑1 (MQ=255)                                                  caccGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTgg                                                                                                 <  2:501963/151‑1 (MQ=255)                                                   accGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGa                                                                                                <  1:814147/151‑1 (MQ=255)                                                    ccGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGaa                                                                                               <  2:236300/151‑1 (MQ=255)                                                     cGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAac                                                                                              <  1:392589/151‑1 (MQ=255)                                                       gTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAacac                                                                                            <  1:857883/151‑1 (MQ=255)                                                            cACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCt                                                                                       >  2:108564/1‑151 (MQ=255)                                                            cACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCt                                                                                       >  1:114741/1‑151 (MQ=255)                                                             aCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTg                                                                                      <  1:729444/151‑1 (MQ=255)                                                              ccATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGc                                                                                     >  1:850753/1‑151 (MQ=255)                                                                aTGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCa                                                                                   <  1:219722/151‑1 (MQ=255)                                                                    cgcgCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAgggt                                                                               >  2:620939/1‑149 (MQ=255)                                                                     gcgcTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGtt                                                                               >  1:323060/1‑150 (MQ=255)                                                                      cgcTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTAc                                                                             <  1:729191/151‑1 (MQ=255)                                                                       gcTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACg                                                                            >  2:147909/1‑151 (MQ=255)                                                                       gcTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACg                                                                            >  1:307943/1‑151 (MQ=255)                                                                           ggAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCa                                                                        <  2:1068293/151‑1 (MQ=255)                                                                            gagaTGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCAc                                                                       <  1:940340/151‑1 (MQ=255)                                                                                 gTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAAc                                                                   <  1:954597/150‑1 (MQ=255)                                                                                 gTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACt                                                                  <  2:739494/151‑1 (MQ=255)                                                                                 gTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACt                                                                  <  1:494046/151‑1 (MQ=255)                                                                                   cGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTc                                                                <  1:883914/151‑1 (MQ=255)                                                                                        gCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGAttt                                                           >  2:42495/1‑151 (MQ=255)                                                                                         ccAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTc                                                          <  1:963625/151‑1 (MQ=255)                                                                                               ccGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGAc                                                    >  1:584876/1‑151 (MQ=255)                                                                                                cggc‑ggGCGCTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACt                                                   <  1:362354/151‑1 (MQ=255)                                                                                                     gggCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCAt                                               >  1:601583/1‑151 (MQ=255)                                                                                                        cACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGc                                            >  1:953881/1‑151 (MQ=255)                                                                                                        cACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGc                                            >  1:353379/1‑151 (MQ=255)                                                                                                           tGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCg                                         >  2:338807/1‑151 (MQ=255)                                                                                                            gTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGccc                                          <  2:961816/149‑1 (MQ=255)                                                                                                               ccAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATa                                     <  1:940711/151‑1 (MQ=255)                                                                                                                 tgTGCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATa                                     >  2:440973/2‑149 (MQ=255)                                                                                                                    gCAACCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCg                                <  1:505220/151‑1 (MQ=255)                                                                                                                      aaCCATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGAAAAACCGCGCGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGa                               >  1:139159/1‑150 (MQ=255)                                                                                                                        ccATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAAcc                            <  2:181347/151‑1 (MQ=255)                                                                                                                        ccATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAAcc                            >  1:758532/1‑151 (MQ=255)                                                                                                                        ccATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAAcc                            <  1:60354/151‑1 (MQ=255)                                                                                                                        ccATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAAcc                            <  2:649844/151‑1 (MQ=255)                                                                                                                         cATTACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCg                           <  2:307952/151‑1 (MQ=255)                                                                                                                            tACCGCGCTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGAt                         <  1:294837/150‑1 (MQ=255)                                                                                                                                gcgcTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGt                    >  2:457125/1‑151 (MQ=255)                                                                                                                                   cTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGc                  >  2:171621/1‑150 (MQ=255)                                                                                                                                   cTCACAATACCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCt                 >  2:587400/1‑151 (MQ=255)                                                                                                                                           aCCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGt         <  1:108554/151‑1 (MQ=255)                                                                                                                                           aCCGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGt         <  2:631055/151‑1 (MQ=255)                                                                                                                                             cGGTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTc       >  1:703669/1‑151 (MQ=255)                                                                                                                                              ggTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCt      <  2:323065/151‑1 (MQ=255)                                                                                                                                               gTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTg     <  2:72774/151‑1 (MQ=255)                                                                                                                                               gTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTg     >  1:556017/1‑151 (MQ=255)                                                                                                                                               gTCAGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTg     <  1:42495/151‑1 (MQ=255)                                                                                                                                                  aGCAGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCgg  >  1:378388/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGC‑GGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGG  >  NC_000913/3400153‑3400447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑