Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	213,341	0	A	G	39.7%	117.6 / 33.1	79	G337G (GGA→GGG)	tilS	tRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (7/40);  new base G (31/0);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 2.37e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCG  >  NC_000913/213205‑213491                                                                                                                                         |                                                                                                                                                          gTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTa                                                                                                                                              <  1:874570/150‑1 (MQ=255) gTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTa                                                                                                                                              <  2:112781/150‑1 (MQ=255)          tGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑g                                                                                                                                    <  2:1003275/150‑1 (MQ=255)           gggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑gg                                                                                                                                   <  2:349609/150‑1 (MQ=255)            ggCGCGTTTGAAATGCGACGCTATCAGGCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCGTAATGCGGGA‑GGCg                                                                                                                                 <  2:840862/151‑1 (MQ=255)               gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAAAGCGGGA‑gggg                                                                                                                                 >  1:1272478/1‑146 (MQ=255)               gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTGCATTCgggg‑gg                                                                                                                                   >  1:903926/1‑146 (MQ=255)                    tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGGGGGTATAGGCTTATGCGGGG‑GGCGATATTTGc                                                                                                                         >  2:1070290/1‑151 (MQ=255)                     gAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGG‑GGCGATATTCGcc                                                                                                                        >  1:1319040/1‑151 (MQ=255)                      aaaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTCATGCGGGG‑GGGGGTATTTGccc                                                                                                                       >  1:1304944/1‑151 (MQ=255)                        aTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTc                                                                                                                     <  2:666689/151‑1 (MQ=255)                         tCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTAATGCGGGG‑GGGGGTATTCGcccccc                                                                                                                    >  1:359905/1‑151 (MQ=255)                          ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTcc                                                                                                                    <  1:230886/150‑1 (MQ=255)                               gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGc                                                                                                              <  1:702449/151‑1 (MQ=255)                               gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGc                                                                                                              <  1:548522/151‑1 (MQ=255)                                  aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCa                                                                                                             <  2:160689/149‑1 (MQ=255)                                    cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAc                                                                                                          <  2:566524/150‑1 (MQ=255)                                     aGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑Ga                                                                                                       <  1:1083433/151‑1 (MQ=255)                                       tCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAAATCGGGG‑GGCGGTATTCGCCCTCCCCCTGCAGAc                                                                                                          >  1:428819/1‑147 (MQ=255)                                        cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGGTTAATGCGGGG‑GGGGGTATTTGCCCCCCGCGTGCAGAC‑Gaa                                                                                                      >  2:151480/1‑149 (MQ=255)                                        cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGc                                                                                                    <  2:739034/151‑1 (MQ=255)                                        cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCGGGGGAGGGTTCAGGCTAAAGCGGGA‑GGGGGTAATTGACCTCCCCCTGCCGAA‑Ga                                                                                                       >  2:675356/1‑148 (MQ=255)                                         gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAATGCGGGG‑GGCGATATTTGCCCTCCGCGGGCAGGC‑GAAGCg                                                                                                   >  1:115523/1‑151 (MQ=255)                                           aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGAAAGGTTTATGCGGGAGGGGGGAATTCG‑CCTCCGCCGGCAGAC‑CAAGCgg                                                                                                  >  1:342525/1‑150 (MQ=255)                                               gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAg                                                                                              <  2:360069/150‑1 (MQ=255)                                               gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAg                                                                                              <  1:759480/150‑1 (MQ=255)                                                    ggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGGGGGGGGCAGCCTTAAGCGGGA‑GGGGGGATTTGCCCTCCCCGCGCAGGC‑GGAGCggggagggtg                                                                                          >  2:268565/1‑141 (MQ=255)                                                    ggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTACAGAC‑GAAGCGGTCAgcgtgcg                                                                                        <  2:656288/151‑1 (MQ=255)                                                        tAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGTACAGCTTTATGCGGGG‑GGCGGTATTCGCCCCCCCCGGGCAGGC‑GAAGCGGGCcgcggggggtt                                                                                     >  2:356021/1‑143 (MQ=255)                                                         aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCa                                                                                   <  2:467106/151‑1 (MQ=255)                                                          aaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGGGTGGAATGGTTAATTCGGGT‑GGGGGTAATCTCCCTCCGCGCGGAAAC‑GAAGCGGGCa                                                                                               >  2:924758/1‑138 (MQ=255)                                                          aaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGGACAGCCTTATGCGGGG‑GGCGATATTTGCCCTCCCCGCGGAGGC‑GAAGGGGGCAGCGGGCGttt                                                                                     >  2:897103/1‑148 (MQ=255)                                                           aTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGTGCGGGGGAGGGGATAACGTAATTCGGGG‑GGGGAGACTCCCCCTCCTCGTGCAGACGGAAGCggctacgcgcgtttcta                                                                                   >  2:819052/1‑135 (MQ=255)                                                             ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAg                                                                                <  1:549548/150‑1 (MQ=255)                                                                 cACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCa                                                                           <  2:538849/151‑1 (MQ=255)                                                                   ccGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAg                                                                          <  2:887210/150‑1 (MQ=255)                                                                        cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGAAAACTTAAAGACGGG‑GGCGAGATTCTGCCCCCCCGTGCCGAA‑GAAACGGGCCGCGTGCGGTTTaaaacccccggggt                                                                      >  2:770472/1‑138 (MQ=255)                                                                        cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGG‑GGCGATATTCGCCCCCCCCGTGCAGAC‑GAAGCGGGCAGCGGGCGGTTTAAAACGCCAGGAtt                                                                      >  1:543758/1‑149 (MQ=255)                                                                        cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAAAGCGGGA‑GGCGATATTTGCCCTCCCCCTGCAGAC‑GAAGCGGGCAGCGGGCGTTTTAAAGCGCCAGGGtt                                                                      >  1:444248/1‑149 (MQ=255)                                                                        cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGAAAGCTTTATGCGGGA‑GGGGGTATTTGCCCCCCCCGGGCCGGC‑GAAGCGGTCAGCGGGCGGTTCaaaagggcagggat                                                                      >  2:519813/1‑138 (MQ=255)                                                                            gCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGCAGAGTGAAAATTTATTGGGGG‑GGCGGTATTCGCCCTCCCCCTGCACCA‑GGAACGGGCAGCGCGCGGTTCTAAGAGCCAGGGtt                                                                      >  1:1243713/1‑145 (MQ=255)                                                                              gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAAGCGGGA‑GGGGATATTTTCCCTCCCCGTGCCGAC‑GAAAAGGGCAGCGGGCGTTTCAAAAAGCCAGGGTTGCTGCat                                                               >  1:1279436/1‑150 (MQ=255)                                                                               aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAAAGCGGGG‑GGGGAGATTTCCCCCCCCCGGGCCGAC‑GAAACGGGGAGCGGGCGTGTCCAAGCGCCCgggatggtggacat                                                             >  2:831937/1‑139 (MQ=255)                                                                                aaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTAATGCGGGG‑GGGGATATTTGCCCCCCCCGGGCAGAC‑GAAGCGGGCAGCGGGGGGTTCAAAACGCCCGGGTTGCTGCATAtt                                                            >  1:923071/1‑151 (MQ=255)                                                                                 aaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCatat                                                             <  1:68981/149‑1 (MQ=255)                                                                                  aCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGt                                                          <  2:378268/151‑1 (MQ=255)                                                                                   cATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAATTCGGGA‑GGCGGTATTCGCCCTCCGCGGGCAGAC‑GAAGCGGGCAGCGGGCGGTTCAAAACGCCCGGGTTGCTGCATAtt                                                            >  1:1282049/1‑148 (MQ=255)                                                                                    aTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCg                                                        <  2:1096232/151‑1 (MQ=255)                                                                                      tgtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCgg                                                       <  1:355857/150‑1 (MQ=255)                                                                                         gCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTTATGCGGGG‑GGGGGTATTCTCCCCCCGCCGGCAGAC‑GAAACGGGCAGCGGGGGGTTCAAAGGGCCAGGATTGCTGCCTATTTTTggg                                                      >  2:544898/1‑148 (MQ=255)                                                                                         gCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGt                                                   <  1:1216142/151‑1 (MQ=255)                                                                                           cGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑aa                                                <  1:924848/151‑1 (MQ=255)                                                                                            gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTAAAGCTTTATGCGGGG‑GGGGATATTTCCCCTCCCCGTGCAGAC‑GAAGAGGGCCGCGGGCGGTTCAAAAGGCCAGGGATGTTGCATATTGTTgggggg                                                   >  2:975863/1‑145 (MQ=255)                                                                                            gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑aa                                                <  2:42248/150‑1 (MQ=255)                                                                                             tGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCCTAATGCGGGA‑GGCGATATTCGCCCCCCGCGTGCCGAC‑GAAGCGGGCAGCGGGCGGTTCAAAGCGCCCGGGTTGCTGCGTAATTTTGGGCggtac                                                >  2:357986/1‑146 (MQ=255)                                                                                             tGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGc                                                     <  2:1075747/145‑1 (MQ=255)                                                                                                 aGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGG‑GGCGGTATTTGCCCTCCCCGGGCAGAC‑GAAAGGGGCAGCGTGCGGTTCAAAACGCCAGGGTTGCTGCATATTGTCGGGGGG‑AAcggggg                                          >  1:57419/1‑151 (MQ=255)                                                                                                   aCGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGAc                                        <  2:717127/151‑1 (MQ=255)                                                                                                   aCGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGAc                                        <  2:1040098/151‑1 (MQ=255)                                                                                                     gTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGt                                      <  2:225459/151‑1 (MQ=255)                                                                                                     gTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGt                                      <  2:1319754/151‑1 (MQ=255)                                                                                                       ggCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTa                                     <  2:962228/150‑1 (MQ=255)                                                                                                         cTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACAGCTTAATTCGGGG‑GGGGATATTTGCCCCCCGCGTGCAGAC‑GAAGCGGGCAGCGGGGGGTTTAAAGAGCCAGGGTTGGTGCCTATTTTCGGGCGG‑AAAGGCGGGCGGaa                                    >  1:1172267/1‑149 (MQ=255)                                                                                                         cTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACCGCTTAATGCGGGG‑GGGGGGATTTTCCCCCCCCGGGCCGAC‑CGAGGGGTCCGCGGGCGGTTTAAAGCGCCCGGGTTGCTGCATATTTTTGGGGGG‑AAaggcgg                                          >  2:1231328/1‑143 (MQ=255)                                                                                                           tCACCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCt                                 <  2:314772/150‑1 (MQ=255)                                                                                                           tCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGGACAGCTTAAATCGGGG‑GGCGATATTTCCCCCCCCCGCGGAGAA‑GAAACGGTGAGCGGGCGTTTTAAAGCGCCCCGGTTGCTGGCTATTGTCGGGGGTGAAcgggggggggaagt                                  >  2:399732/1‑142 (MQ=255)                                                                                                            cAACCGCTGGAATTACCGGCGGGGGTGGGAAGTGTACAGCTTAATGCGGGG‑GGGGATATTCGCCCCCCGCGGGCCGAC‑GAAGCGGTCAGCGGGCGGTTTAAAACGCCAGGGTTGCTGCATATTGTTGGGCGG‑AAAGGCGGGAGTAAGCTa                                >  1:45384/1‑150 (MQ=255)                                                                                                                 gCTGGAATTACCGGCGGGGCTGGGGAATGTAAAGCTTGATTCGGGG‑GGGGGTATTCGCCCTCCCCGGGGAGAC‑GAAAAGGTCAGCGTGCGGTTTTAAAAGCCCGGGTTGCCGCATATTTTTGGGGGG‑ATAGGCGGGCGGAAGCGaaa                              >  2:928651/1‑147 (MQ=255)                                                                                                                   tGGAATTACCGGCGGGGCCTGGGAGGGTACAGCCTAATACGGGG‑GGCGATATTTGCCCTCCCCGTGCAGAC‑GAAGCGGGTAGCGGGGGTGTCTAAACGCCAGGATTGCTGCATATTGGTGGGCGT‑AACGGCGGGCGGAAACGaaaaaaaa                         >  2:379185/1‑150 (MQ=255)                                                                                                                    ggAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGaaaa                         <  1:560124/149‑1 (MQ=255)                                                                                                                           ccGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAag                <  1:185246/151‑1 (MQ=255)                                                                                                                               cggGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCt            <  2:735471/151‑1 (MQ=255)                                                                                                                                ggggCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTg           >  1:1032924/1‑151 (MQ=255)                                                                                                                                 gggCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTgg          >  1:320918/1‑151 (MQ=255)                                                                                                                                   gCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTggg         >  2:692456/1‑150 (MQ=255)                                                                                                                                   gCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGc        <  2:446652/151‑1 (MQ=255)                                                                                                                                     tGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCg       >  1:819343/1‑150 (MQ=255)                                                                                                                                      gggAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGt      <  2:429011/150‑1 (MQ=255)                                                                                                                                      gggAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCGCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGCACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTg     <  2:642577/151‑1 (MQ=255)                                                                                                                                         aaGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgccg  >  2:305954/1‑151 (MQ=255)                                                                                                                                         aaGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgccg  <  2:543981/151‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                          GTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGA‑GGCGATATTCGCCCTCCGCGTGCAGAC‑GAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGT‑AACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCG  >  NC_000913/213205‑213491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑