| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,890 | 0 | C | A | 56.7% | 12.4 / 38.6 | 30 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (8/5); new base A (0/17); total (8/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.20e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.64e-04 | |||||||||||
GCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCT > NC_000913/357747‑358032 | gCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAAccg > 1:362792/1‑151 (MQ=255) gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAAccgc < 1:433418/150‑1 (MQ=255) cccATCAGCCAATCCCAGCCCCAGACCTGTTTCAGCGGCTACCGTGATTCATTCCCGCC‑AA‑CACCCGCGCATTTATCCAACGCCATGTGCAAAATTGCCTCGGCGGCGGCTGTCTGCCAGTTATTTTT‑CCGCCGCAACAAAAccgccg < 2:8652/146‑1 (MQ=18) cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTcc > 1:689215/1‑151 (MQ=255) aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg > 2:455066/1‑151 (MQ=255) gCCCCACACTTGTGTCTGCGCCTCCCGTAATT‑ATTTCCCCCAAA‑CAGCCGCCCTTTTTCCCACCTCAATGTAAAAAAATCCTTGCGGGGGGGTTGTCGCCCGGTTTTTTCC‑CCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGc < 1:131827/148‑1 (MQ=255) cGCAGCCTTGTGTCAGGGCCTACCGTGTTTCATTCCCCCC‑AA‑CACCCGCGCATTTACCCACCGCCTTGTCAAAAAATGCTTGCGGGGGGGCTGTCTGCCGGTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc < 1:73522/151‑1 (MQ=255) cAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:923837/1‑148 (MQ=255) cgtgtgTCAGCGGCTCCGGTGATTCTTTCCCCCC‑AA‑CACCCGCGCTTTTACCCACCGCCATGTGAAAAAAGGCTTCCGGGGCGGCTTTCTGCCAGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAag < 1:558397/146‑1 (MQ=255) gtCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:468941/1‑139 (MQ=255) gtCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAaggg > 1:270815/1‑143 (MQ=255) cctttattctttCCCCC‑AACCACCCGCCCATTTACCCACCCCCTGGGGAAAAAATCCCTCGGCGGCGGCTGTCTGCCAGTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCaa < 2:837379/122‑1 (MQ=255) ttCATTCCCCCC‑AA‑CACCCGCGCTTTTACCCACCGCATTGTGAAAAAATGCTTCCGGGGCGCCTGTCTGCCAGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑a < 1:922392/148‑1 (MQ=255) ctttcccccc‑AA‑CACCCGCGTTTTTACCCACCCCCATGGGAAAAATTCCCTTCGCGGCGGTTGTCT‑CCAGTTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑At < 1:267735/139‑1 (MQ=255) cccccc‑AA‑CACCCGCCATTTTACCCACCGCCTTGTCAAAAAATGCCTCGGGGGCGGCTGTCGGCCAGTTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTtgtgtg < 1:1278190/147‑1 (MQ=255) aa‑caccCGCCCATTTACCCACCCCCATGTAAAAAAAGGCTTCCGGGGCGGCTGTCGGCCAGCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATc < 1:896938/151‑1 (MQ=255) gcgcTTTTACCAAACCCTATGTAAAAAAATCCTTGCGCGGGGGCTGTCTGCCAGTTTTTTTT‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCgctg < 1:1196114/148‑1 (MQ=255) caaacccgtgggcaaaaaaTGCCTCCGGGGCGGCTGTCTGCAAGTTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAAt < 2:1323813/135‑1 (MQ=255) accgcttgggacAAAAATGCCTCGGCGGCGGCTGTCTGCCAGTTATTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAATGTgg < 2:187722/140‑1 (MQ=255) gtgAAAAAATCCCTTCGGGGCGGCTGTCTGCCAGTTTTTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAggg < 2:979972/149‑1 (MQ=255) ggcaaaaaaTGCCTCCGCGGGGGTTGCCGGCGAGTTTTTTCC‑CCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGaa < 2:310983/147‑1 (MQ=255) aaaatTGCCTCCGCGCGGGCTTTCTGCCAGTTATTTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGaa < 1:79548/147‑1 (MQ=255) aaaaTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAAGTTTAAAACCCTCAATGTT‑TTTGGGGATAGCTGCTGGTTACAACGTgg > 2:783339/1‑138 (MQ=255) aattGCCTCCGGGGCGCCTTTCCGCCGTTTTTTTTC‑CCGCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcgg < 2:24848/148‑1 (MQ=255) ggcggtTGTCTCCCGCTTTTTTTT‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAAt < 2:1329885/144‑1 (MQ=255) cTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAAATTTTAAACCCCC‑ATGTTTTGTGGGGAACGCTGCCGGGGACAAAATGGGAAGGGGAGGGCGGGGGAACAACTCCCGaa > 1:1253634/1‑150 (MQ=255) ccAGCTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgcg < 1:506326/148‑1 (MQ=255) ttttttc‑CCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAtt < 1:1153583/147‑1 (MQ=255) ttcccgccgCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAACGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGtt < 1:810575/147‑1 (MQ=255) ccccgaAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCt < 1:19340/149‑1 (MQ=255) aacaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAAATTTTAAACCCCC‑CTGTTTTTTGGGGATCGCCGCTGGGAAAAAAATGGGAAGGGGAGGGGGGGGGAGCAGCTCCCGAAACGCGCCAAATTAGttt > 1:1158706/1‑140 (MQ=255) | GCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTC‑ATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCT > NC_000913/357747‑358032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |