Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,495,028	T→C	H15R (CAC→CGC)	ydcI ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,495,028	0	T	C	100.0%	306.2 / NA	80	H15R (CAC→CGC)	ydcI	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/45);  total (35/45)

TGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAG  >  NC_000913/1494884‑1495174                                                                                                                                                 |                                                                                                                                                   tGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGgcgc                                                                                                                                               >  2:603684/1‑150 (MQ=255)  gacgacCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCa                                                                                                                                              >  2:358878/1‑150 (MQ=255)   acgacCACGCTCAAACACGCGAGCGCCAGTCATCTGGTCCAGTTCATTCACTGTCTTAGAGAGCGCCGGTTGGCTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTTTGGGACAGCTACGAATGTATGAAGgcggcgaa                                                                                                                                              >  2:943582/1‑147 (MQ=255)   acgacCACGCGCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATGCAATGTCTTAGAGAGCGCAGGTTGACTCAAATGAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTTTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCa                                                                                                                                              <  2:161476/149‑1 (MQ=255)    cgacCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAAc                                                                                                                                           >  1:1197610/1‑151 (MQ=255)     gacCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACg                                                                                                                                          >  2:1215082/1‑151 (MQ=255)     gacCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACg                                                                                                                                          <  2:161992/151‑1 (MQ=255)        cACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGAt                                                                                                                                       <  1:916814/151‑1 (MQ=255)        cACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGAt                                                                                                                                       <  2:59418/151‑1 (MQ=255)           gCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATgcg                                                                                                                                    <  2:736156/151‑1 (MQ=255)                aaCAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGAc                                                                                                                               <  1:1031773/151‑1 (MQ=255)                aaCAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGAc                                                                                                                               >  1:495696/1‑151 (MQ=255)                 aCAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACt                                                                                                                              >  1:1265156/1‑151 (MQ=255)                  cAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACt                                                                                                                              >  1:1265810/1‑150 (MQ=255)                   aaGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTaa                                                                                                                            <  1:938078/151‑1 (MQ=255)                    agcgagcgCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTaaa                                                                                                                           <  2:648596/151‑1 (MQ=255)                      cgagcgCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAAc                                                                                                                          >  2:719785/1‑150 (MQ=255)                        agcgCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAAc                                                                                                                          >  2:1150887/1‑148 (MQ=255)                        agcgCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAAc                                                                                                                          >  2:1060840/1‑148 (MQ=255)                          cgcCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACt                                                                                                                     <  2:946618/151‑1 (MQ=255)                             cAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTAtt                                                                                                                  <  2:696419/151‑1 (MQ=255)                              aGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTAttt                                                                                                                 <  1:362968/151‑1 (MQ=255)                               gTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTAtttt                                                                                                                <  1:211935/151‑1 (MQ=255)                                 cAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTAttttt                                                                                                               <  1:880773/150‑1 (MQ=255)                                    ctgctCCAGTTCATTCAATGTCTTAAAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTcc                                                                                                           >  1:573355/1‑151 (MQ=255)                                      gctCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCa                                                                                                          <  2:281320/150‑1 (MQ=255)                                      gctCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCAt                                                                                                         <  1:725255/151‑1 (MQ=255)                                          cAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGc                                                                                                     <  1:1069498/151‑1 (MQ=255)                                              tcattcaATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGAt                                                                                                  <  1:502925/150‑1 (MQ=255)                                              tcattcaATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGAt                                                                                                  <  1:304153/150‑1 (MQ=255)                                                    aaTGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTaaaa                                                                                           <  2:342118/151‑1 (MQ=255)                                                     aTGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTaaaaa                                                                                          <  1:96827/151‑1 (MQ=255)                                                        tCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACg                                                                                        >  2:1131324/1‑150 (MQ=255)                                                                  cgcAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCg                                                                             <  1:690873/151‑1 (MQ=255)                                                                      ggTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTg                                                                          >  2:34471/1‑150 (MQ=255)                                                                        ttGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGAc                                                                        >  2:559759/1‑150 (MQ=255)                                                                           aCTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGt                                                                    >  2:553319/1‑151 (MQ=255)                                                                              cAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGaa                                                                 <  2:938892/151‑1 (MQ=255)                                                                               aaaTTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGa                                                                  >  1:107856/1‑149 (MQ=255)                                                                                 aTTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAgtt                                                              <  1:1080856/151‑1 (MQ=255)                                                                                  ttAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAgttg                                                             >  1:436736/1‑151 (MQ=255)                                                                                     aGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGttt                                                          <  1:152697/151‑1 (MQ=255)                                                                                     aGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGttt                                                          >  2:883030/1‑151 (MQ=255)                                                                                     aGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGttt                                                          <  2:1151771/151‑1 (MQ=255)                                                                                      gggTTTCAGACGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGGCTAAACTGACTATTTTTGTCCATAATGGATGTTAAAAGCGAAGCGGTGTCGCTGACAAGTGAAgttgtc                                                           <  2:1250623/149‑2 (MQ=255)                                                                                            cAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATg                                                   <  1:433622/151‑1 (MQ=255)                                                                                            cAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATg                                                   <  1:794119/151‑1 (MQ=255)                                                                                             aGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATg                                                   >  2:456604/1‑150 (MQ=255)                                                                                              gCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGa                                                  <  2:411056/150‑1 (MQ=255)                                                                                              gCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGAt                                                 <  2:587405/151‑1 (MQ=255)                                                                                               ccGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATa                                                <  2:549726/151‑1 (MQ=255)                                                                                               ccGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATa                                                <  1:325342/151‑1 (MQ=255)                                                                                                    cgcCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTg                                           <  1:778765/151‑1 (MQ=255)                                                                                                    cgcCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTg                                           >  1:243707/1‑151 (MQ=255)                                                                                                     gcCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTg                                           >  2:334708/1‑150 (MQ=255)                                                                                                     gcCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTg                                           >  2:773649/1‑150 (MQ=255)                                                                                                      ccccAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGAt                                         <  1:90255/151‑1 (MQ=255)                                                                                                       cccAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGAtt                                        >  2:796939/1‑151 (MQ=255)                                                                                                        ccAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTg                                       >  1:868725/1‑151 (MQ=255)                                                                                                               tCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAat                                 <  1:962964/150‑1 (MQ=255)                                                                                                                 cTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAAtatt                              >  1:1093570/1‑151 (MQ=255)                                                                                                                   tgttgtGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAAtatta                             <  1:882970/150‑1 (MQ=255)                                                                                                                    gttgtGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAAtattat                            <  1:45975/150‑1 (MQ=255)                                                                                                                    gttgtGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATattatt                           >  2:165737/1‑151 (MQ=255)                                                                                                                    gttgtGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATattatt                           <  1:34817/151‑1 (MQ=255)                                                                                                                    gttgtGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATattatt                           <  1:934190/151‑1 (MQ=255)                                                                                                                       gtgCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATAttattagc                        <  2:694174/151‑3 (MQ=255)                                                                                                                           gACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAAtt                    <  1:498280/151‑1 (MQ=255)                                                                                                                            aCAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAAtt                    >  2:576398/1‑150 (MQ=255)                                                                                                                              aGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTaaa                 <  2:582346/151‑1 (MQ=255)                                                                                                                              aGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTaa                  >  1:955485/1‑150 (MQ=255)                                                                                                                                   cGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAAt            >  1:1259336/1‑151 (MQ=255)                                                                                                                                   cGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAAt            >  2:1315159/1‑151 (MQ=255)                                                                                                                                   cGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAAt            <  1:817676/151‑1 (MQ=255)                                                                                                                                      aTGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTAt         >  1:297201/1‑151 (MQ=255)                                                                                                                                      aTGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTAt         >  2:1279734/1‑151 (MQ=255)                                                                                                                                         tATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGtt      >  2:164219/1‑151 (MQ=255)                                                                                                                                          aTGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGtt      >  2:911377/1‑150 (MQ=255)                                                                                                                                           tGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTAc    >  1:513789/1‑151 (MQ=255)                                                                                                                                            gAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTAct   <  1:1214409/151‑2 (MQ=255)                                                                                                                                             aaGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAg  <  2:102168/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAG  >  NC_000913/1494884‑1495174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑