Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,660	0	G	T	43.1%	106.5 / 29.6	58	E321* (GAA→TAA)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base G (5/28);  new base T (25/0);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 8.35e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACG  >  NC_000913/2252512‑2252808                                                                                                                                                     |                                                                                                                                                            gCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑a                                                                                                                                                           <  1:1019210/150‑1 (MQ=255)  cAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAg                                                                                                                                                         <  1:599291/151‑1 (MQ=255)   aaCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATTACTTTCTTT‑aa                                                                                                                                                          >  1:368906/1‑149 (MQ=255)         ttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAAGTTTTTTT‑TAGGA‑GACa                                                                                                                                                  >  2:530831/1‑150 (MQ=255)          tgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAg                                                                                                                                                 <  1:113205/150‑1 (MQ=255)                    cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑a                                                                                                                                     <  1:1007946/151‑1 (MQ=255)                    cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑a                                                                                                                                     <  1:1036003/151‑1 (MQ=255)                      cGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAACTTTTTTT‑TAGGA‑AAAAGttt                                                                                                                                              >  1:606135/1‑141 (MQ=255)                         tGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGTCTCGGAGATATCTTTCTTT‑TAGGG‑GATAATTTCCGCCGC‑Agccgc                                                                                                                                >  1:517288/1‑148 (MQ=255)                            gTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATTTAGCTTTTTTT‑AAGGA‑TAAAGTTTTCCCGCC‑AGCG‑ccc                                                                                                                              >  1:502060/1‑149 (MQ=255)                              cAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATTACTTTTTTT‑TAGGA‑GACAGTTTTCCCCAC‑AGCG‑ccccgtt                                                                                                                          >  1:797127/1‑147 (MQ=255)                               aGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAACTTTTTTT‑TAGGA‑AAAAGTTTTCGCGAC‑CGCG‑CCCGcttt                                                                                                                         >  1:938708/1‑151 (MQ=255)                                    aTTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGt                                                                                                                    <  2:1037821/151‑1 (MQ=255)                                      tGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTa                                                                                                                   <  1:616518/150‑1 (MQ=255)                                       gCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGc                                                                                                                 <  1:839307/151‑1 (MQ=255)                                         aGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGGTATAAGTTTTTTT‑TAGGG‑TAAAATTTTCGCGGC‑AACG‑CCCGtttttggggag                                                                                                                  >  1:808933/1‑141 (MQ=255)                                           cGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTgc                                                                                                             <  1:781679/151‑1 (MQ=255)                                            gAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAACTTTTTTT‑TAGGA‑TACAGGTTTCCCGGC‑AGCG‑CCCCttttttgggaggc                                                                                                                >  1:344340/1‑138 (MQ=255)                                               aaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGCTTTTTTT‑TAGGA‑TACAGTTTTCGCGCC‑AGCG‑CCCCGTTTTTGGTAGccctggcgg                                                                                                         >  2:768982/1‑144 (MQ=255)                                                   aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAAGTTTTTTT‑TAGGG‑TAAAAGTTTCGCGGC‑AGCG‑CCCGCTTTTCGGTAGGCCGCGC‑gggca                                                                                                     >  2:313629/1‑147 (MQ=255)                                                    tGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATTACTTTTTTT‑ATAGA‑AACAATTTTCGCCGC‑AGCG‑CCCGCTTTGCGGGGGCCTTCGC‑ggggaat                                                                                                   >  1:729826/1‑146 (MQ=255)                                                        gaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTc‑gc                                                                                             <  1:402180/151‑1 (MQ=255)                                                         aaCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTATAGCTTTTTTT‑TAGGG‑TAAAGTTTTCCCGAC‑CGCG‑CCCCGTTTTTGGGAGCCCGCGC‑GG‑CGAttttg                                                                                               >  1:282159/1‑147 (MQ=255)                                                           cAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTCGGGTATAGGTTTTTTT‑TAGGG‑AAAAGTTTTCGCGCCAACCC‑CCCGTTTTGGGGGGGCCT‑CGC‑GG‑GAAatt                                                                                                 >  2:647397/1‑145 (MQ=255)                                                            aaTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑gcgcgc                                                                                         <  1:506090/151‑1 (MQ=255)                                                             aTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGCTTTTTTT‑TAGGG‑TACAGTTTTCGCCCC‑ACCG‑CCCGCTTTTTCGTTGCCCGCGC‑GG‑CCACTTT‑TCGCGcc                                                                                        >  2:222733/1‑151 (MQ=255)                                                                      gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAg                                                                                <  2:1000973/150‑1 (MQ=255)                                                                         cgcgCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAACTTTTTTT‑TTGGG‑GAAAAGTTTCTCGCC‑AGCG‑CCCCCTTTGTGGGGGACTGTGC‑GG‑CCATTTT‑GCGCGccccccg                                                                                   >  2:273059/1‑139 (MQ=255)                                                                          gcgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATTGCTTTTTTT‑TAGGA‑TACAGTTTTCGCGAC‑AGCG‑CCCCTTTTTCGGTTGGCTGCGC‑GG‑CAATTTT‑GCGCGCCCGCGGCAGCtt                                                                             >  1:473778/1‑149 (MQ=255)                                                                           cgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGTATATAACTTTTTTT‑TAGGA‑TAAAGTTTTCGCCCC‑AACG‑CCCGTTTTTCGGTAGCCTGCGC‑GG‑CGATTTT‑TCGCGcc                                                                                        >  1:280787/1‑137 (MQ=255)                                                                            gcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAaac                                                                         <  2:87406/151‑1 (MQ=255)                                                                              cAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAACTTTTTTT‑TAGGA‑TACAAGTTTCGCGCC‑AGCG‑CCCCGTTTTCGGGAGCCCGCGC‑GG‑CGAATTT‑GCGCGCCCGCGGCGGCCTaaa                                                                          >  1:448299/1‑148 (MQ=255)                                                                                    aTACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAGATTTTTTT‑TAGGG‑GAAAGGTTTTGCGAC‑AGCG‑CCCCGTTTGTGGTAGGCTGCGC‑GG‑GGATTTT‑GCGCGCCCGCGGCCGCTTAAACAaacc                                                                    >  2:633388/1‑148 (MQ=255)                                                                                    aTACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAATTTTTTTGTAGGGA‑AAAAGTTT‑CTCGGC‑AGCC‑CCCCCTTTTGGGGAGGCTGCGCGGG‑CAATTT‑‑TCCCGCCCGCGGCCGCCTTAACAaacc                                                                    >  2:175575/1‑148 (MQ=255)                                                                                      aCGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAaccctacc                                                                <  1:874762/150‑1 (MQ=255)                                                                                       cGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAACTTTTTTT‑TAGGAGAAAAGTTTCC‑CGAC‑AGCG‑CCCGCTTTTTGGGAGGCCGCGC‑GG‑GGAATTT‑GCGCGCCCGCGGCCGCTTAAAAAaacccaccc                                                               >  1:736281/1‑150 (MQ=255)                                                                                         aCAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGt                                                            <  2:448173/151‑1 (MQ=255)                                                                                         aCAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGt                                                            <  2:223102/151‑1 (MQ=255)                                                                                            gTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGGTATAGCATTTTTT‑TAGGA‑GAAAGGTTTCCCGGC‑AGCGCCCCGTTTTTGGGT‑GCCTGCGC‑CG‑CGATTTT‑TCGCGCCCGCGGGCGGTTAAAAAACACCACCCCGTc                                                           >  2:505932/1‑149 (MQ=255)                                                                                                  aGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATa                                                   <  2:736083/151‑1 (MQ=255)                                                                                                       tGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAAcgc                                               <  1:439698/150‑1 (MQ=255)                                                                                                        ggTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAAcgcg                                              <  2:353242/150‑1 (MQ=255)                                                                                                           gCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCttt                                          <  2:368811/151‑1 (MQ=255)                                                                                                           gCTGACGATGGTTTTATGGCGGGCCTGGGATATAACTTTCTTT‑AAAGA‑TAAAGTTTTCGCGAC‑AGCG‑CCCGCTTTTCGGTAGCCCGCGC‑GG‑CAATTTC‑GCGCGCCCGCGGCAGCTTAAAAAAACCCACCCCGTCTGCCGATAACGCGCctt                                          >  1:970875/1‑151 (MQ=255)                                                                                                            cTGACGATGGTTTTATGGCGGGCCCTGGATATAGTTTTTTTT‑ATGGA‑TAAAGTTTTCTCGGC‑AGCG‑CCCGCCTTTTTGTGGGCCCCGC‑GGCCAATTTCTGCGCCCCAG‑GCAAGCTTAAA‑AACCCCACCCCCTCTGcccgaaaacggcct                                           >  2:641787/1‑137 (MQ=255)                                                                                                              gACGATGGTTTTATGGCGGGCCCGGGATATAGATTTTTTT‑TAGGA‑TACAGTTTTCGCGGC‑AACG‑CCCGCTTTTTGGGAGCCTGCGC‑GG‑CGAATTT‑GCGCGCCAGCGGCAGCCTAAACAAAACCACCCCCTCTGTCCCTAACGCGCttt                                          >  1:298775/1‑148 (MQ=255)                                                                                                                 gATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAg                                    <  2:992576/151‑1 (MQ=255)                                                                                                                  aTGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGt                                   <  1:474804/151‑1 (MQ=255)                                                                                                                    ggTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGc                                 <  2:517146/151‑1 (MQ=255)                                                                                                                          aTGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATg                            <  2:103278/150‑1 (MQ=255)                                                                                                                          aTGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATg                            <  2:961591/150‑1 (MQ=255)                                                                                                                            ggCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgtg                         >  2:770954/1‑151 (MQ=255)                                                                                                                                        atataGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTCC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg              <  1:957655/150‑1 (MQ=255)                                                                                                                                             gCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGAt        >  2:416549/1‑151 (MQ=255)                                                                                                                                                ttCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCg     >  2:32085/1‑151 (MQ=255)                                                                                                                                                ttCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCg     >  2:282624/1‑151 (MQ=255)                                                                                                                                                 tCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCg     <  1:424352/150‑1 (MQ=255)                                                                                                                                                    tG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACg  <  2:282088/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                            GCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTG‑AAGGA‑TACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GG‑CAATTTC‑GCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACG  >  NC_000913/2252512‑2252808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑