Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,441	0	A	C	46.8%	72.1 / 11.4	47	G384G (GGT→GGG)	yqeF	short chain acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (21/4);  new base C (0/22);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.47e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGC  >  NC_000913/2984296‑2984591                                                                                                                                                  |                                                                                                                                                       atGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCt                                                                                                                                                    <  1:884942/150‑1 (MQ=255)  tGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTgg                                                                                                                                                  >  2:346532/1‑151 (MQ=255)   gTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTgg                                                                                                                                                  <  2:343984/150‑1 (MQ=255)        gagaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGccccc                                                                                                                                             <  2:120899/150‑1 (MQ=255)         agaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgc                                                                                                                                           <  2:29850/151‑1 (MQ=255)                      gAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGt                                                                                                                              >  1:795529/1‑151 (MQ=255)                                  aaatTGTATGTCAATTTGCCACCCATCATTATTCAAAAAATTAAGGACGGAAGTTATTTATCGGTAAGGAGAAAATGCAGAGCTTTTCGTCACTTTCAATGGTCATTGCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCtt                                                                                                                   <  1:596820/150‑1 (MQ=255)                                       gtatgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCggg                                                                                                             >  2:434113/1‑151 (MQ=255)                                          tgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt                                                                                                          >  2:168845/1‑151 (MQ=255)                                                 tggccacccaTAATTATTAAAAAGATTAAGGACGGCAGATATTTATCGGAAAGGAGAAAATGAAGAGCTTTTCGTCACGTTAAATGGTCAAGCCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGt                                                                                                     <  2:391961/147‑1 (MQ=255)                                                  tGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTc                                                                                                  >  2:1027445/1‑151 (MQ=255)                                                    ccagccaTAATTATTCAACAGATTAATGACGGAAGATTTTTATGGGAAAGGAGAAAATGCAGAGCTATTCGTCACGTTCAATGTTCAATCCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGttt                                                                                                   <  2:240777/148‑1 (MQ=255)                                                       gccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCAt                                                                                             >  2:267923/1‑151 (MQ=255)                                                       gccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCAt                                                                                             >  1:959000/1‑151 (MQ=255)                                                           tcatTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTc                                                                                          >  2:486219/1‑150 (MQ=255)                                                              ttattCAACAAATTAAGGACGGCAGATTTTTATCGGTAAGGAGAAAATGCAGAGTTTTTCGTCCCTTTCAATGGTCAATCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCa                                                                                              <  1:346626/143‑1 (MQ=255)                                                                    aaCAGATTAATGACGGCAGATATTTAGCGGTAAGGAGAAAATGCAGAGCTTTTCGTCACGTTCAATGGTCAATGCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAAct                                                                                   <  1:246771/148‑2 (MQ=255)                                                                     aCAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCag                                                                                 >  1:827232/1‑149 (MQ=255)                                                                           tAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAcc                                                                          >  2:168370/1‑150 (MQ=255)                                                                           aaaTGACGGCAGATTTTTATGGGTAGGGAGAAAAGGAAGAGTTTTTCTCCACTTTCAGTGGTCAATCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAcc                                                                          <  1:678838/149‑1 (MQ=255)                                                                             aTGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAgg                                                                       >  2:159318/1‑151 (MQ=255)                                                                                  ggaaGATATTTAGCGATAAGGAGGAAAGGCAGAGCTTTTCGTCACGTTAAATGGTCAACCCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGAt                                                                     <  2:943467/145‑1 (MQ=255)                                                                                  ggCAGATATTTATCGGTAAGGAGGAAATGAAGAGCTTTTCGTCACTTTAAATGGTCAACCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCg                                                                  <  1:134784/151‑1 (MQ=255)                                                                                     aGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGc                                                                >  1:767905/1‑150 (MQ=255)                                                                                          tttATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg                                                          >  2:240583/1‑151 (MQ=255)                                                                                          tttATCGGTAAGGAGAAAATGAAGACCTATTCGTCACGTTCAATGGTCAATGCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAAcc                                                            <  1:621161/149‑1 (MQ=255)                                                                                          tttATCGGAAAGGAGGAAATGCAGACCTTTTCGTCACTTTAAAGGGTCAAGGCCCCCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAACCCAGGATTCGGCAAc                                                             <  1:248931/148‑1 (MQ=255)                                                                                          tttAGCGGAAAGGAGGAAATGCAGAGCTTTTCGTCCCGTTAAATGGTCATTCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCa                                                               <  2:321452/146‑1 (MQ=255)                                                                                           ttATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg                                                          >  2:636778/1‑150 (MQ=255)                                                                                           ttAGCGGAAAGGAGAAAATGCAGAGTTTTTCGTCACTTTCAATGGTCAATGCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg                                                          <  1:624817/150‑1 (MQ=255)                                                                                            tATCGGAAAGGAGAAAATGCAGATTTATTCGTCACGTCAAAGGGTCAATGCCCCCCCCGCGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg                                                          <  2:976367/149‑1 (MQ=255)                                                                                             aTCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGa                                                         >  1:948979/1‑149 (MQ=255)                                                                                              tgggTAAGGAGGAAATGAGGAGTTTTTCTTCACGTAAAATGGTCAACCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGa                                                         <  2:761694/146‑1 (MQ=255)                                                                                                ggTAAGGAGGAAATGCAGAGTTTTTTGTCACGTTAAATGGTCAATGCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg                                                       <  1:487913/148‑1 (MQ=255)                                                                                                ggTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgcgc                                                    >  2:85939/1‑151 (MQ=255)                                                                                                       aggaAATGAAGAGCTTTTCGTCACGTTAAATGTTCATTCCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCg                                              <  1:113954/150‑1 (MQ=255)                                                                                                          aatcgaagagTTATTCGTCCCCTTCAGTGGTAAAGCCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCggg                                            <  1:396252/143‑1 (MQ=255)                                                                                                             tGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGTGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGAc                                        >  1:386456/1‑150 (MQ=255)                                                                                                                agagTTTTTCGTCACGTTAAATGGTCAATCCCACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGa                                     <  2:157124/150‑1 (MQ=255)                                                                                                                  accTATTCGTCACTTTCAATGGTCAATCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTg                                  <  2:827020/149‑1 (MQ=255)                                                                                                                            cACTTTAAATGGTCAATCCCACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATcg                            <  1:482720/147‑1 (MQ=255)                                                                                                                             aCGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGcc                        >  2:511715/1‑150 (MQ=255)                                                                                                                                ttCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGATACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGccacc                     >  2:478251/1‑150 (MQ=255)                                                                                                                                   aaTGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattg                 >  2:22027/1‑151 (MQ=255)                                                                                                                                   aaTGGTAAATGCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattg                 <  1:807696/151‑1 (MQ=255)                                                                                                                                    aTGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGCGACCGAGTGCGATCGCGCCACcattg                 >  2:970708/1‑150 (MQ=255)                                                                                                                                       gTCAATCCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgacat             <  2:795325/151‑1 (MQ=255)                                                                                                                                                acacCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGAc    >  1:22176/1‑151 (MQ=255)                                                                                                                                                acacCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGAc    >  1:178628/1‑151 (MQ=255)                                                                                                                                                 cacCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTAGCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGAc    <  1:558410/150‑1 (MQ=255)                                                                                                                                                  acCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGc  >  2:795387/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                       ATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGC  >  NC_000913/2984296‑2984591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑