Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,495,028	T→C	H15R (CAC→CGC)	ydcI ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,495,028	0	T	C	100.0%	320.7 / NA	81	H15R (CAC→CGC)	ydcI	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/43);  total (38/43)

CCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTAC  >  NC_000913/1494880‑1495172                                                                                                                                                     |                                                                                                                                                 ccccTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCgg                                                                                                                                                >  2:556125/1‑151 (MQ=255)   ccTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGgcggcg                                                                                                                                              >  1:120723/1‑151 (MQ=255)    cTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGgcgc                                                                                                                                             >  2:911114/1‑151 (MQ=255)    cTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGgcgc                                                                                                                                             >  1:599721/1‑151 (MQ=255)      gacgacCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCa                                                                                                                                            >  1:277535/1‑150 (MQ=255)                cTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATgcgc                                                                                                                                 >  1:543661/1‑151 (MQ=255)                  cAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCt                                                                                                                                <  2:1007460/150‑1 (MQ=255)                   aaaCAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGa                                                                                                                              <  1:177387/151‑1 (MQ=255)                    aaCAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGAc                                                                                                                             >  1:379278/1‑151 (MQ=255)                       aaGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTaa                                                                                                                          <  2:31801/151‑1 (MQ=255)                       aaGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTaa                                                                                                                          <  1:228503/151‑1 (MQ=255)                         gcgagcgCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAAc                                                                                                                        >  2:56914/1‑151 (MQ=255)                         gcgagcgCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAAc                                                                                                                        >  1:201149/1‑151 (MQ=255)                           gagcgCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAAc                                                                                                                        >  1:348640/1‑149 (MQ=255)                             gcgcCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGAc                                                                                                                    <  1:998028/151‑1 (MQ=255)                             gcgcCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGAc                                                                                                                    >  2:784010/1‑151 (MQ=255)                                  aGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTAttt                                                                                                               <  1:928250/151‑1 (MQ=255)                                     cAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTAtttttt                                                                                                            <  2:677551/151‑1 (MQ=255)                                     cAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTAtttttt                                                                                                            <  2:875428/151‑1 (MQ=255)                                      aGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTAttttttt                                                                                                           <  1:710193/151‑1 (MQ=255)                                      aGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTAttttttt                                                                                                           >  1:251978/1‑151 (MQ=255)                                      aGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTAttttttt                                                                                                           >  2:921769/1‑151 (MQ=255)                                         tgctCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTcc                                                                                                         >  2:1020431/1‑150 (MQ=255)                                          gctCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTcc                                                                                                         <  1:909365/149‑1 (MQ=255)                                           ctCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTcc                                                                                                         <  2:742911/148‑1 (MQ=255)                                           ctCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCAt                                                                                                       >  2:946056/1‑150 (MQ=255)                                              cAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGATTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGc                                                                                                   >  1:893210/1‑151 (MQ=255)                                                 ttcattcaATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGAt                                                                                                >  1:213093/1‑151 (MQ=255)                                                 ttcattcaATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGAt                                                                                                >  1:829664/1‑151 (MQ=255)                                                        aaTGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTaaaa                                                                                         <  2:439435/151‑1 (MQ=255)                                                             cTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGa                                                                                     >  1:894415/1‑150 (MQ=255)                                                               tAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGaa                                                                                    <  1:733420/149‑1 (MQ=255)                                                               tAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAg                                                                                   <  2:63241/150‑1 (MQ=255)                                                               tAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGc                                                                                  >  2:672469/1‑151 (MQ=255)                                                                    agCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGgtgt                                                                             <  1:946290/151‑1 (MQ=255)                                                                        cAGGTTGACTCAAATTAATGGTTTCAGCCGCGCGCCCCAAAGTTCCTTTTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTc                                                                            <  1:744182/148‑1 (MQ=255)                                                                        cAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCt                                                                         <  2:760508/151‑1 (MQ=255)                                                                         aGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTg                                                                        <  2:543513/151‑1 (MQ=255)                                                                           gTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGAc                                                                      <  2:399771/151‑1 (MQ=255)                                                                             tGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACa                                                                     >  1:1007831/1‑150 (MQ=255)                                                                                cTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTg                                                                 >  2:581738/1‑151 (MQ=255)                                                                                   aaaTTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGa                                                                >  1:338180/1‑149 (MQ=255)                                                                                    aaTTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAgt                                                             >  1:208079/1‑151 (MQ=255)                                                                                     aTTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAgtt                                                            <  1:825981/151‑1 (MQ=255)                                                                                     aTTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAgtt                                                            <  2:51504/151‑1 (MQ=255)                                                                                     aTTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAgtt                                                            <  2:412922/151‑1 (MQ=255)                                                                                     aTTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAgtt                                                            <  2:868373/151‑1 (MQ=255)                                                                                        aaGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAgttgtt                                                         >  1:678798/1‑151 (MQ=255)                                                                                          gggTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTg                                                       <  2:618136/151‑1 (MQ=255)                                                                                           ggTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTg                                                       >  1:395361/1‑150 (MQ=255)                                                                                             tttCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGAtt                                                    >  2:206777/1‑151 (MQ=255)                                                                                             tttCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGAtt                                                    >  1:789979/1‑151 (MQ=255)                                                                                             tttCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTT‑AAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGAtt                                                    >  1:538194/1‑150 (MQ=255)                                                                                                cAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTAt                                                  <  1:640409/150‑1 (MQ=255)                                                                                                cAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTAt                                                  <  1:275492/150‑1 (MQ=255)                                                                                                cAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATg                                                 <  2:599549/151‑1 (MQ=255)                                                                                                 aGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATg                                                 >  2:743571/1‑150 (MQ=255)                                                                                                 aGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGa                                                <  1:109188/151‑1 (MQ=255)                                                                                                 aGCCGCGCGCCCCAAAGGGCCTTGTTGGGCGACAGCTACGAATGTTTGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTGACATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATg                                                 <  2:619086/150‑1 (MQ=255)                                                                                                   ccGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATa                                              <  1:964629/151‑1 (MQ=255)                                                                                                      cgcgcCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTc                                                                            >  2:163624/1‑118 (MQ=255)                                                                                                      cgcgcCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTc                                                                            <  1:163666/118‑1 (MQ=255)                                                                                                       gcgcCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACtt                                          >  2:227168/1‑151 (MQ=255)                                                                                                       gcgcCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACtt                                          <  2:870235/151‑1 (MQ=255)                                                                                                         gcCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTg                                         >  2:909185/1‑150 (MQ=255)                                                                                                          ccccAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGAt                                       >  2:618613/1‑151 (MQ=255)                                                                                                           cccAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGAtt                                      >  1:231589/1‑151 (MQ=255)                                                                                                              aaaGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCa                                   <  1:249033/151‑1 (MQ=255)                                                                                                               aaGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCa                                   >  1:867052/1‑150 (MQ=255)                                                                                                                 gTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCaaaa                                <  2:507505/151‑1 (MQ=255)                                                                                                                   tCCTTGTTGTGCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAat                               <  1:349884/150‑1 (MQ=255)                                                                                                                           gtgCGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTaa                       <  2:32815/150‑1 (MQ=255)                                                                                                                              cGACAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAAt                   <  2:864617/151‑1 (MQ=255)                                                                                                                                 cAGCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTa                 >  2:434928/1‑150 (MQ=255)                                                                                                                                   gCTACGAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAg              <  1:922001/151‑1 (MQ=255)                                                                                                                                        gAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAAtt         >  1:695490/1‑151 (MQ=255)                                                                                                                                        gAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAAtt         <  2:1024583/151‑1 (MQ=255)                                                                                                                                        gAATGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAAt          <  2:529253/150‑1 (MQ=255)                                                                                                                                         aaTGTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTa        <  2:120694/151‑1 (MQ=255)                                                                                                                                            gTATGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGt     <  2:710910/151‑1 (MQ=255)                                                                                                                                              aTGAAGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGtt    <  1:363375/150‑1 (MQ=255)                                                                                                                                                 aaGGCGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTAc  >  1:771407/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTAC  >  NC_000913/1494880‑1495172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑