Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,282	0	T	G	51.8%	67.9 / 84.7	54	V526G (GTT→GGT)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (5/21);  new base G (28/0);  total (33/21)
Fisher's exact test for biased strand distribution p-value = 1.26e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-10

TGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGC  >  NC_000913/354140‑354430                                                                                                                                               |                                                                                                                                                      tGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTgggg                                                                                                                                                  >  1:213843/1‑148 (MQ=255)  ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGa                                                                                                                                               <  1:27835/150‑1 (MQ=255)   gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaaa                                                                                                                                             <  1:372733/151‑1 (MQ=255)    ggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTggggggga                                                                                                                                              >  1:704479/1‑145 (MQ=255)      cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGAt                                                                                                                                          <  2:356407/151‑1 (MQ=255)       aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATg                                                                                                                                         <  1:743190/151‑1 (MQ=255)       aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGGAAGGTg                                                                                                                                         >  1:248965/1‑151 (MQ=255)            tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTggggggttaagtggggtg                                                                                                                                    >  2:549341/1‑137 (MQ=255)                  tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCT‑GGAAggg                                                                                                                             >  1:203961/1‑151 (MQ=255)                        ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGGAAAATGTGCT‑GAAAGGGGAggtg                                                                                                                       >  2:516146/1‑151 (MQ=255)                         tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGCAggtgg                                                                                                                      >  2:752641/1‑151 (MQ=255)                          aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtgg                                                                                                                      <  1:44322/150‑1 (MQ=255)                              cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGCCgggggcgggg                                                                                                                 >  2:90534/1‑149 (MQ=255)                               tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCC‑GGAAggggggggggggg                                                                                                                   >  1:558168/1‑138 (MQ=255)                                 tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGGAgggggcggggggg                                                                                                              >  2:931179/1‑149 (MQ=255)                                       cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GGAAGGGGGGGGGGGGGGGGCGgtt                                                                                                           >  1:866622/1‑148 (MQ=255)                                        gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGGGCT‑GGAAGGGCAggggggggggggggtt                                                                                                           >  1:22024/1‑142 (MQ=255)                                            gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTcc                                                                                                   <  1:219208/151‑1 (MQ=255)                                               cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAAGGGAGGTGGCGGGGGCGGTTGTCATTTCGa                                                                                                 >  2:314567/1‑150 (MQ=255)                                                  aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa                                                                                                <  1:431921/148‑1 (MQ=255)                                                              aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCt                                                                                 <  2:31351/151‑1 (MQ=255)                                                              aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGCGGGGGGGTTTGTCCTTTCGAAAAAGAGCGGAAGTCt                                                                                 >  1:965381/1‑151 (MQ=255)                                                                gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTg                                                                                <  2:354823/150‑1 (MQ=255)                                                                 ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTGGGGGGGGAAGAAGCGCTGGAAAGGGC‑GGGGGGGGGGGGGATTGGCATTTCGGAagagcgggagcggaccgg                                                                              >  1:234858/1‑137 (MQ=255)                                                                  tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGa                                                                              <  2:409943/150‑1 (MQ=255)                                                                   gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                             <  1:610810/150‑1 (MQ=255)                                                                   gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                            <  2:808001/151‑1 (MQ=255)                                                                   gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAAAAGAGCT‑GAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGGGCGACAGTCTgg                                                                               >  2:818730/1‑148 (MQ=255)                                                                         cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGTGGCGGGGGGGGTTTTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCggg                                                                      >  2:782351/1‑149 (MQ=255)                                                                          aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTTATTTCGGAAGAGAGCGACCGTCTGGAAGATCg                                                                        >  2:70003/1‑148 (MQ=255)                                                                          aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCT‑GGAAAGGGAGGGGGGGGGGGGGGTTGTTATTCCGAAAGAGAGGGACAGGCTGGGAGGTc                                                                         >  1:855569/1‑147 (MQ=255)                                                                              gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCT‑GAAAGGGGCGGGGGGGGGGGGGGTTTTCATTTCCAAAAAGAGCGACAGGCTGGAAGGTCGTGAtg                                                                   >  1:554865/1‑149 (MQ=255)                                                                                     aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGGAGATCGTGATGTGGCGCAc                                                           >  2:260692/1‑150 (MQ=255)                                                                                      cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                         <  2:279023/151‑1 (MQ=255)                                                                                          cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCT‑GAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCCGAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGGGCACTCGc                                                       >  1:602529/1‑149 (MQ=255)                                                                                          cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCC‑GAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTccc                                                       >  1:389955/1‑147 (MQ=255)                                                                                          cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTTGAAGATCGTGATGTGGCGCACTc                                                         >  2:688523/1‑147 (MQ=255)                                                                                           gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGGAGGTGGCGGGGGCGTTTGGCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATTTGGCGCACTCGc                                                       >  2:119816/1‑148 (MQ=255)                                                                                            agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAaga                                                   <  2:902200/151‑1 (MQ=255)                                                                                            agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGGTTGTCATTTCGAAAGAGAGCGACAGGCTGGGAGATCGTGATGTGGCGCCCTc                                                         >  1:160050/1‑145 (MQ=255)                                                                                              aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCC‑GGAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGGAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGCGCACTCGcca                                                     >  1:961356/1‑145 (MQ=255)                                                                                                          aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                      <  2:736242/150‑1 (MQ=255)                                                                                                            ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGGAAGATGCGCT‑GGAAAGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAAATCGTGGTGTGGCGCACTCGCAAGAGAAGGGGATTATGgcg                                   >  2:336972/1‑151 (MQ=255)                                                                                                               cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtg                                <  2:380212/151‑1 (MQ=255)                                                                                                               cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtg                                <  2:334444/151‑1 (MQ=255)                                                                                                                  ccATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGCGGTTGTCCTTCCGGAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCCCTCGCAAGAGAAGGCGGTTATGGCGCtgggg                             >  1:495411/1‑149 (MQ=255)                                                                                                                   cATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                            >  1:769472/1‑151 (MQ=255)                                                                                                                      ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAc                          <  2:129892/150‑1 (MQ=255)                                                                                                                       cGGGCGTTGCCGAAGTGGCGGAGGGTGGGGTGGAAAATGCGCT‑GAAAAGGGAGGGGGCGGTGGGGGTTGTCGTTTCGAAAAAAAACGACAGTCTGGGAGAACGGGGTGTGGGGGACTCGCGAGAGAAAGCGAATATGGCGCTGGGGGAc                          >  1:96040/1‑149 (MQ=255)                                                                                                                                   aaGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCa             >  1:137012/1‑150 (MQ=255)                                                                                                                                   aaGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCa             >  2:791016/1‑150 (MQ=255)                                                                                                                                        gCGGTGGTTGGGGTGAAAGATGCTCT‑CAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGCGGCCCACTCGCATGAGAAGGCGATTATGGCCCTGGTGGACAGCCTGATCGGCAACtttc       <  1:549333/151‑2 (MQ=255)                                                                                                                                        gCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg       <  2:635342/151‑1 (MQ=255)                                                                                                                                         cGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg      <  1:90530/151‑1 (MQ=255)                                                                                                                                             ggtTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc  >  1:121810/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                      TGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGC  >  NC_000913/354140‑354430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑