Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	218,835	(A)8→7	intergenic (‑60/+52)	proS ← / ← tsaA	prolyl‑tRNA synthetase/tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	218,828	0	A	.	100.0%	376.8 / NA	79	intergenic (‑53/+59)	proS/tsaA	prolyl‑tRNA synthetase/tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (41/38);  total (41/38)

GGATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGG  >  NC_000913/218679‑218972                                                                                                                                                      |                                                                                                                                                 ggATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaa                                                                                                                                                 <  1:781790/151‑1 (MQ=255) ggATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaa                                                                                                                                                 <  1:1171168/151‑1 (MQ=255) ggATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACa                                                                                                                                                  <  2:199056/150‑1 (MQ=255) ggATCAGCCCAGCGCGCAGCCTCAGCTGATGGCTGATCACCTGGCCGTCGGCAGGTGTCTCCTTGAGAGGGGAGAGCAGGTATTGGCTAGTACGCAGGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGGCCAAGGCGAGCCTGGGAGaa                                                                                                                                                 <  2:344071/151‑1 (MQ=255)     catcCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaaaa                                                                                                                                             <  2:78867/151‑1 (MQ=255)     catcCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaaaa                                                                                                                                             <  2:641534/151‑1 (MQ=255)     catcCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaaa                                                                                                                                              <  1:419177/150‑1 (MQ=255)     catcCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaaa                                                                                                                                              <  1:248844/150‑1 (MQ=255)        cccTGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCCTCGGCAGGCGTCTCCTTGAGAGTGGAGAGCAGGAATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaa                                                                                                                                                >  2:941245/1‑145 (MQ=255)          cGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaaaaagtga                                                                                                                                        <  2:704449/151‑5 (MQ=255)           ggCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGAt                                                                                                                                      <  1:356386/151‑1 (MQ=255)             cgcgcAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGAttt                                                                                                                                    >  1:29461/1‑151 (MQ=255)             cgcgcAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGAttt                                                                                                                                    >  2:140222/1‑151 (MQ=255)              gcgcAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTa                                                                                                                                   <  1:708860/151‑1 (MQ=255)                gcagcaTCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGt                                                                                                                                 <  1:842507/151‑1 (MQ=255)                 cagcaTCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGtt                                                                                                                                <  2:1089526/151‑1 (MQ=255)                     aTCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTAcc                                                                                                                            >  1:687975/1‑151 (MQ=255)                     aTCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTAcc                                                                                                                            >  2:801206/1‑151 (MQ=255)                        aGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGt                                                                                                                         <  2:1117024/151‑1 (MQ=255)                        aGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGt                                                                                                                         <  2:217697/151‑1 (MQ=255)                               ggCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCaaaa                                                                                                                   <  1:1112892/150‑1 (MQ=255)                               ggCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCaaaa                                                                                                                   <  1:428941/150‑1 (MQ=255)                               ggCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCaaa                                                                                                                    >  2:491733/1‑149 (MQ=255)                                    aTCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATg                                                                                                             >  1:351006/1‑151 (MQ=255)                                        ccTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCaa                                                                                                         <  2:480437/151‑1 (MQ=255)                                        ccTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCaa                                                                                                         <  1:1201434/151‑1 (MQ=255)                                         cTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCaa                                                                                                         >  1:415706/1‑150 (MQ=255)                                           cGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCaaaa                                                                                                       <  1:792856/150‑1 (MQ=255)                                               gTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAgaga                                                                                                   >  1:282983/1‑150 (MQ=255)                                                 cGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAg                                                                                                 <  1:342262/150‑1 (MQ=255)                                                         gtCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAAt                                                                                        <  1:741285/151‑1 (MQ=255)                                                           ctcCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGCGAAGGGCGTGAAttt                                                                                      >  2:52112/1‑151 (MQ=255)                                                           ctcCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAAttt                                                                                      >  1:862639/1‑151 (MQ=255)                                                             ccTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAAttt                                                                                      <  1:269618/149‑1 (MQ=255)                                                             ccTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTaa                                                                                    <  1:655157/151‑1 (MQ=255)                                                               ttGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAAcg                                                                                  >  1:208623/1‑151 (MQ=255)                                                                tGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAAcgc                                                                                 >  1:1123197/1‑151 (MQ=255)                                                                tGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAAc                                                                                   <  1:1047002/149‑1 (MQ=255)                                                                 gagaGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAAcgcg                                                                                >  2:343498/1‑151 (MQ=255)                                                                      tGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGtt                                                                             <  1:729371/149‑1 (MQ=255)                                                                         agagCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAg                                                                         >  1:865565/1‑150 (MQ=255)                                                                              aGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCaa                                                                    >  1:1171181/1‑150 (MQ=255)                                                                               agTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCaa                                                                    >  1:331289/2‑149 (MQ=255)                                                                                 tATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGAc                                                                >  1:705816/1‑151 (MQ=255)                                                                                 tATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGAc                                                                >  1:1137633/1‑151 (MQ=255)                                                                                   ttGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACtt                                                              >  2:354886/1‑151 (MQ=255)                                                                                    tGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTc                                                             >  1:150348/1‑151 (MQ=255)                                                                                    tGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTc                                                             >  1:839751/1‑151 (MQ=255)                                                                                      gCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCa                                                            >  1:1201019/1‑150 (MQ=255)                                                                                      gCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCa                                                            >  1:128090/1‑150 (MQ=255)                                                                                         aGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAAc                                                        >  1:1099290/1‑151 (MQ=255)                                                                                          gTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAAcc                                                       <  1:620299/151‑1 (MQ=255)                                                                                           tACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCt                                                      >  1:544306/1‑151 (MQ=255)                                                                                            aCGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTg                                                     >  1:226489/1‑151 (MQ=255)                                                                                               cATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGt                                                  <  2:150353/151‑1 (MQ=255)                                                                                                     gttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGGCTTCAAAACCTGCGTCGGTGa                                            <  2:1146535/151‑1 (MQ=255)                                                                                                     gttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGa                                            <  2:882590/151‑1 (MQ=255)                                                                                                      ttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGa                                            >  2:979177/1‑150 (MQ=255)                                                                                                         cGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGAcgcg                                        <  2:882944/151‑1 (MQ=255)                                                                                                         cGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGAcgcg                                        <  1:123997/151‑1 (MQ=255)                                                                                                          ggTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACgcgc                                       >  2:1009373/1‑151 (MQ=255)                                                                                                           gTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGcc                                      >  2:902001/1‑151 (MQ=255)                                                                                                             tCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGcc                                      >  2:82141/1‑149 (MQ=255)                                                                                                              ccAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGc                                   <  1:793797/151‑1 (MQ=255)                                                                                                               cAGTTGGAAGGTATAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGACTTAGTTTACCAGTGCATAAGAAATGTCAAAAGAAAAGGGCGTGAATTTAACGTGGTTCCAGCGCAAACACTTCAAAACCTGCGTCGATCACGCGcctg                                    >  2:1235894/1‑147 (MQ=255)                                                                                                               cAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCg                                  <  1:659789/151‑1 (MQ=255)                                                                                                               cAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCg                                  <  1:1244185/151‑1 (MQ=255)                                                                                                                aGTTGGAAGGTAGAACGGGCTCAAGGCGAGCCTGGGAC‑CAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCg                                  >  2:565625/1‑150 (MQ=255)                                                                                                                 gTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGaa                                <  2:194956/151‑1 (MQ=255)                                                                                                                 gTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGa                                 >  1:15296/1‑150 (MQ=255)                                                                                                                    ggAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAAAGt                             >  2:886090/1‑151 (MQ=255)                                                                                                                     gAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGtt                            >  2:94567/1‑151 (MQ=255)                                                                                                                       aGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaa                          <  1:1137137/151‑1 (MQ=255)                                                                                                                        ggTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaaa                         <  2:29456/151‑1 (MQ=255)                                                                                                                         gTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaaaa                        <  1:883022/151‑1 (MQ=255)                                                                                                                           aGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATc                      <  2:145573/151‑1 (MQ=255)                                                                                                                              aCAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATg                   <  2:1042099/151‑1 (MQ=255)                                                                                                                               cAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGt                  >  2:117868/1‑151 (MQ=255)                                                                                                                                aGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGt                  >  1:236655/1‑150 (MQ=255)                                                                                                                                 ggCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAg                <  2:586602/151‑1 (MQ=255)                                                                                                                                     cAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCa             <  1:1013545/150‑1 (MQ=255)                                                                                                                                     cAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAg            <  2:1192025/151‑1 (MQ=255)                                                                                                                                      aaGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGa           <  2:1102985/151‑1 (MQ=255)                                                                                                                                          cGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGc       <  1:635440/151‑1 (MQ=255)                                                                                                                                               cTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAgg  >  1:616869/1‑151 (MQ=255)                                                                                                                                               cTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAgg  >  1:62970/1‑151 (MQ=255)                                                                                                                                                tGGGAC‑AAAAAAAGTGCTTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAgg  >  2:818108/1‑150 (MQ=255)                                                                                                                                                tGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGAc                                           <  2:578706/109‑1 (MQ=255)                                                                                                                                                tGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGAc                                           >  1:578730/1‑109 (MQ=255)                                                                                                                                                      |                                                                                                                                                 GGATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGG  >  NC_000913/218679‑218972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑