Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,594,053	(C)6→7	pseudogene (73/1461 nt)	yneO ←	pseudogene, AidA homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,594,047	1	.	C	100.0%	174.8 / NA	46	pseudogene (79/1461 nt)	yneO	pseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/22);  total (24/22)

TATCAACCCGAACGCGAGTGTTCCCTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTT‑CCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTAT  >  NC_000913/1593897‑1594191                                                                                                                                                        |                                                                                                                                                 tATCAACCCGAACGCGAGTGTTCCCTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGttt                                                                                                                                                   >  2:869573/1‑151 (MQ=255)  aTCAACCCGAACGCGAGTGTTCCCTTGGGTATCGCGTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTGCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGttt                                                                                                                                                   <  2:1180263/150‑1 (MQ=255)          gAACGCGAGTGTTCCCTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTT‑ccccccctt                                                                                                                                         >  1:121343/1‑148 (MQ=255)          gAACGCGAGTGTTCCCTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTT‑ccccccctt                                                                                                                                         >  1:953253/1‑148 (MQ=255)          gAACGCGAGTGTTCCCTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTT‑ccccccctt                                                                                                                                         <  2:291649/151‑4 (MQ=255)           aaCGCGAGTGTTCCCTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTT‑cccccccttc                                                                                                                                        <  2:954661/151‑5 (MQ=255)           aaCGCGAGTGTTCCCTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTT‑cccccccttc                                                                                                                                        <  2:336399/151‑5 (MQ=255)                gAGTGTTCCCTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTAt                                                                                                                                    <  2:692752/151‑1 (MQ=255)                   tgtTCCCTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGCACGTCATTAGGCCATTGTTTCCGGGATAGTTGCCGTTAACGGTCCGTACATTCACCGGTTTCCCCCCCTTCGCTa                                                                                                                                     >  2:455321/1‑147 (MQ=255)                    gtTCCCTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCAt                                                                                                                                >  2:1141048/1‑151 (MQ=255)                        ccTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGa                                                                                                                            <  1:88908/151‑1 (MQ=255)                         cTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGa                                                                                                                            >  1:324517/1‑150 (MQ=255)                         cTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGa                                                                                                                            >  1:212469/1‑150 (MQ=255)                          ttGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAAc                                                                                                                          >  2:1156838/1‑151 (MQ=255)                            ggctATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAAcc                                                                                                                         <  2:223678/147‑1 (MQ=255)                             ggTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGt                                                                                                                       >  1:278920/1‑151 (MQ=255)                             ggTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGt                                                                                                                       >  2:554946/1‑151 (MQ=255)                              gTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTg                                                                                                                      <  1:354121/151‑1 (MQ=255)                               tATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTgc                                                                                                                     >  2:105301/1‑151 (MQ=255)                                    ccTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCa                                                                                                                <  2:350338/151‑1 (MQ=255)                                       ttCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCAt                                                                                                               <  1:475572/149‑1 (MQ=255)                                       ttCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCAt                                                                                                               <  2:643963/149‑1 (MQ=255)                                               cATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGtt                                                                                                     >  2:179318/1‑151 (MQ=255)                                                        ggTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTGGCCGTTAACGGTCAGTCCATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGAt                                                                                            <  2:121344/151‑1 (MQ=255)                                                         gTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGAt                                                                                            >  2:1062464/1‑150 (MQ=255)                                                         gTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATa                                                                                           <  1:1047945/151‑1 (MQ=255)                                                         gTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATa                                                                                           <  2:108410/151‑1 (MQ=255)                                                           gggCGAATTATCGCCGCCCAGCGTCGCTTTGAACGTCATCAGGCCATTGTTTCCGTTATAGTTGCCGTTAACGGTCAGTACATTCCCCGATTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGt                                                                                         <  2:829132/151‑1 (MQ=255)                                                                  ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGAATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGtttt                                                                                   >  1:323589/1‑150 (MQ=255)                                                                        ccgccCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGac                                                                             >  2:1035547/1‑150 (MQ=255)                                                                        ccgccCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGac                                                                             >  2:913999/1‑150 (MQ=255)                                                                        ccgccCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGac                                                                             <  1:704643/150‑1 (MQ=255)                                                                           cccAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGc                                                                         <  2:375740/151‑1 (MQ=255)                                                                                cGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGc                                                                    >  1:15396/1‑151 (MQ=255)                                                                                  tCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGccc                                                                  >  1:447309/1‑151 (MQ=255)                                                                                     cgTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGccccc                                                                <  2:443484/150‑1 (MQ=255)                                                                                      gTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGccccccc                                                              >  2:59117/1‑151 (MQ=255)                                                                                               catcaGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATaa                                                     <  1:196754/150‑1 (MQ=255)                                                                                                     gCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCat                                              <  1:1223987/151‑1 (MQ=255)                                                                                                       cATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCatat                                            <  1:105302/151‑1 (MQ=255)                                                                                                              tCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGc                                     >  2:849782/1‑151 (MQ=255)                                                                                                               ccGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGc                                     >  2:1172486/1‑150 (MQ=255)                                                                                                               ccGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCa                                    <  1:593348/151‑1 (MQ=255)                                                                                                                cGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGc                                     >  1:131673/1‑149 (MQ=255)                                                                                                                   tataGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAgtg                                 >  2:471784/1‑150 (MQ=255)                                                                                                                            cGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTc                       >  1:438013/1‑151 (MQ=255)                                                                                                                            cGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTc                       <  1:1024953/151‑1 (MQ=255)                                                                                                                                aaCGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCg                    <  1:789075/150‑1 (MQ=255)                                                                                                                                aaCGGTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGa                   <  1:783973/151‑1 (MQ=255)                                                                                                                                   ggTCAGTACATTCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACAt                >  1:955588/1‑151 (MQ=255)                                                                                                                                              tCCCCGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGt     >  1:219460/1‑151 (MQ=255)                                                                                                                                               ccccGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGt     <  2:953250/150‑1 (MQ=255)                                                                                                                                                 ccGGTTTCCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTAt  >  1:1075505/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                 TATCAACCCGAACGCGAGTGTTCCCTTGGGTATCGCCTTTCACGTTCATCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTT‑CCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTAT  >  NC_000913/1593897‑1594191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑